| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Animal Science Cornell University Ithaca, NY 14853
Corresponding author:
Dale E. Bauman; E-mail:
deb6{at}cornell.edu.
Feeding conjugated linoleic acid (CLA) reduces milk fat synthesis in lactating dairy cows, and the effect has been shown to be specific for the trans-10, cis-12 CLA isomer. Our objectives were to examine potential mechanisms by which trans-10, cis-12 CLA inhibits milk fat synthesis. Multiparous Holstein cows (n = 4) in late lactation were used in a balanced 2 x 2 crossover design. Treatments consisted of a 5 d abomasal infusion of either skim milk (control) or purified trans-10, cis-12 CLA (13.6 g/d) emulsified in skim milk. On d 5 of infusion, mammary gland biopsies were performed and a portion of the tissue analyzed for mRNA expression of acetyl CoA carboxylase, fatty acid synthetase, 
9-desaturase, lipoprotein lipase, fatty acid binding protein, glycerol phosphate acyltransferase and acylglycerol phosphate acyltransferase. Lipogenic capacity was evaluated with another portion of the tissue. Infusion of trans-10, cis-12 CLA decreased milk fat content and yield 42 and 48%, respectively and increased the trans-10, cis-12 CLA content in milk fat from <0.1 to 4.9 mg/g. Reductions in milk fat content of C4 to C16 fatty acids contributed 63% to the total decrease in milk fat yield (molar basis). Analysis of the ratios of specific fatty acid pairs indicated trans-10, cis-12 CLA also shifted fatty acid composition in a manner consistent with a reduction in 9-desaturase. Mammary explant incubations with radiolabeled acetate established that lipogenic capacity was decreased 82% and acetate oxidation to CO2 was reduced 61% when cows received trans-10, cis-12 CLA. Infusing trans-10, cis-12 CLA also decreased the mRNA expression of all measured enzymes by 39 to 54%. Overall, data demonstrated the mechanism by which trans-10, cis-12 CLA inhibits milk fat synthesis includes decreasing expression of genes that encode for enzyme involved in circulating fatty acid uptake and transport, de novo fatty acid synthesis, desaturation of fatty acids and triglyceride synthesis.
Abbreviation key: ACC = acetyl CoA carboxylase, AGPAT = acylglycerol phosphate acyltransferase, CLA = conjugated linoleic acid, FAS = fatty acid synthetase, FABP = fatty acid binding protein, GPAT = glycerol phosphate acyltransferase, LPL = lipoprotein lipase, PPAR = peroxisome proliferator-activated receptor, SCD = stearoyl CoA desaturase, SREBP = sterol regulatory element-binding proteins
Key Words: conjugated linoleic acid • lactation • milk fat • milk fat depression
This article has been cited by other articles:
|
|
 |
|
  R. Gervais and P. Y. Chouinard Effects of Intravenous Infusion of Conjugated Diene 18:3 Isomers on Milk Fat Synthesis in Lactating Dairy Cows J Dairy Sci, September 1, 2008; 91(9): 3568 - 3578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Glasser, A. Ferlay, M. Doreau, P. Schmidely, D. Sauvant, and Y. Chilliard Long-Chain Fatty Acid Metabolism in Dairy Cows: A Meta-Analysis of Milk Fatty Acid Yield in Relation to Duodenal Flows and De Novo Synthesis J Dairy Sci, July 1, 2008; 91(7): 2771 - 2785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bharathan, D. J. Schingoethe, A. R. Hippen, K. F. Kalscheur, M. L. Gibson, and K. Karges Conjugated Linoleic Acid Increases in Milk from Cows Fed Condensed Corn Distillers Solubles and Fish Oil J Dairy Sci, July 1, 2008; 91(7): 2796 - 2807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. G. Kadegowda, L. S. Piperova, P. Delmonte, and R. A. Erdman Abomasal Infusion of Butterfat Increases Milk Fat in Lactating Dairy Cows J Dairy Sci, June 1, 2008; 91(6): 2370 - 2379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Abrahamse, J. Dijkstra, B. Vlaeminck, and S. Tamminga Frequent Allocation of Rotationally Grazed Dairy Cows Changes Grazing Behavior and Improves Productivity J Dairy Sci, May 1, 2008; 91(5): 2033 - 2045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Shingfield, A. Arola, S. Ahvenjarvi, A. Vanhatalo, V. Toivonen, J. M. Griinari, and P. Huhtanen Ruminal Infusions of Cobalt-EDTA Reduce Mammary {Delta}9-Desaturase Index and Alter Milk Fatty Acid Composition in Lactating Cows J. Nutr., April 1, 2008; 138(4): 710 - 717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Corl, S. A. Mathews Oliver, X. Lin, W. T. Oliver, Y. Ma, R. J. Harrell, and J. Odle Conjugated Linoleic Acid Reduces Body Fat Accretion and Lipogenic Gene Expression in Neonatal Pigs Fed Low- or High-Fat Formulas J. Nutr., March 1, 2008; 138(3): 449 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Flowers, S. A. Ibrahim, and A. A. AbuGhazaleh Milk Fatty Acid Composition of Grazing Dairy Cows When Supplemented with Linseed Oil J Dairy Sci, February 1, 2008; 91(2): 722 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Bauman, J. W. Perfield II, K. J. Harvatine, and L. H. Baumgard Regulation of Fat Synthesis by Conjugated Linoleic Acid: Lactation and the Ruminant Model J. Nutr., February 1, 2008; 138(2): 403 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Fitzgerald, E. L. Annen-Dawson, L. H. Baumgard, and R. J. Collier Evaluation of Continuous Lactation and Increased Milking Frequency on Milk Production and Mammary Cell Turnover in Primiparous Holstein Cows J Dairy Sci, December 1, 2007; 90(12): 5483 - 5489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hurtaud and J. L. Peyraud Effects of Feeding Camelina (Seeds or Meal) on Milk Fatty Acid Composition and Butter Spreadability J Dairy Sci, November 1, 2007; 90(11): 5134 - 5145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. AbuGhazaleh, D. O. Felton, and S. A. Ibrahim Milk Conjugated Linoleic Acid Response to Fish Oil and Sunflower Oil Supplementation to Dairy Cows Managed Under Two Feeding Systems J Dairy Sci, October 1, 2007; 90(10): 4763 - 4769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. N Close, D. A Schoeller, A. C Watras, and E. H Nora Conjugated linoleic acid supplementation alters the 6-mo change in fat oxidation during sleep Am. J. Clinical Nutrition, September 1, 2007; 86(3): 797 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Feng, A. M. Salter, T. Parr, and P. C. Garnsworthy Extraction and Quantitative Analysis of Stearoyl-Coenzyme A Desaturase mRNA from Dairy Cow Milk Somatic Cells J Dairy Sci, September 1, 2007; 90(9): 4128 - 4136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Odens, R. Burgos, M. Innocenti, M. J. VanBaale, and L. H. Baumgard Effects of Varying Doses of Supplemental Conjugated Linoleic Acid on Production and Energetic Variables During the Transition Period J Dairy Sci, January 1, 2007; 90(1): 293 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Wynn, Z. C. T. R. Daniel, C. L. Flux, J. Craigon, A. M. Salter, and P. J. Buttery Effect of feeding rumen-protected conjugated linoleic acid on carcass characteristics and fatty acid composition of sheep tissues J Anim Sci, December 1, 2006; 84(12): 3440 - 3450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Liu, S. C. Degner, and D. F. Romagnolo Trans-10, Cis-12 Conjugated Linoleic Acid Inhibits Prolactin-Induced Cytosolic NADP+-Dependent Isocitrate Dehydrogenase Expression in Bovine Mammary Epithelial Cells J. Nutr., November 1, 2006; 136(11): 2743 - 2747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Harvatine and D. E. Bauman SREBP1 and Thyroid Hormone Responsive Spot 14 (S14) Are Involved in the Regulation of Bovine Mammary Lipid Synthesis during Diet-Induced Milk Fat Depression and Treatment with CLA J. Nutr., October 1, 2006; 136(10): 2468 - 2474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Murrieta, B. W. Hess, E. J. Scholljegerdes, T. E. Engle, K. L. Hossner, G. E. Moss, and D. C. Rule Evaluation of milk somatic cells as a source of mRNA for study of lipogenesis in the mammary gland of lactating beef cows supplemented with dietary high-linoleate safflower seeds J Anim Sci, September 1, 2006; 84(9): 2399 - 2405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Badinga and E. S. Greene Physiological Properties of Conjugated Linoleic Acid and Implications for Human Health Nutr Clin Pract, August 1, 2006; 21(4): 367 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Perfield II, P. Delmonte, A. L. Lock, M. P. Yurawecz, and D. E. Bauman Trans-10, trans-12 conjugated linoleic acid does not affect milk fat yield but reduces delta9-desaturase index in dairy cows. J Dairy Sci, July 1, 2006; 89(7): 2559 - 2566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Soder, M. A. Sanderson, J. L. Stack, and L. D. Muller Intake and performance of lactating cows grazing diverse forage mixtures. J Dairy Sci, June 1, 2006; 89(6): 2158 - 2167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Drackley, S. S. Donkin, and C. K. Reynolds Major advances in fundamental dairy cattle nutrition. J Dairy Sci, April 1, 2006; 89(4): 1324 - 1336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Harvatine and M. S. Allen Effects of Fatty Acid supplements on milk yield and energy balance of lactating dairy cows. J Dairy Sci, March 1, 2006; 89(3): 1081 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Harvatine and M. S. Allen Effects of Fatty Acid supplements on feed intake, and feeding and chewing behavior of lactating dairy cows. J Dairy Sci, March 1, 2006; 89(3): 1104 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E Mosley, A. L Wright, M. K McGuire, and M. A McGuire trans Fatty acids in milk produced by women in the United States Am. J. Clinical Nutrition, December 1, 2005; 82(6): 1292 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Reveneau, C. V. D. M. Ribeiro, M. L. Eastridge, N. R. St-Pierre, and J. L. Firkins Processing Whole Cottonseed Moderates Fatty Acid Metabolism and Improves Performance by Dairy Cows J Dairy Sci, December 1, 2005; 88(12): 4342 - 4355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. Soita, M. Fehr, D. A Christensen, and T. Mutsvangwa Effects of Corn Silage Particle Length and Forage:Concentrate Ratio on Milk Fatty Acid Composition in Dairy Cows Fed Supplemental Flaxseed J Dairy Sci, August 1, 2005; 88(8): 2813 - 2819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Moore, J. K. Kay, R. J. Collier, M. J. VanBaale, and L. H. Baumgard Effect of Supplemental Conjugated Linoleic Acids on Heat-Stressed Brown Swiss and Holstein Cows J Dairy Sci, May 1, 2005; 88(5): 1732 - 1740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ringseis, D. Saal, A. Muller, H. Steinhart, and K. Eder Dietary Conjugated Linoleic Acids Lower the Triacylglycerol Concentration in the Milk of Lactating Rats and Impair the Growth and Increase the Mortality of their Suckling Pups J. Nutr., December 1, 2004; 134(12): 3327 - 3334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Peterson, E. A. Matitashvili, and D. E. Bauman The Inhibitory Effect of trans-10, cis-12 CLA on Lipid Synthesis in Bovine Mammary Epithelial Cells Involves Reduced Proteolytic Activation of the Transcription Factor SREBP-1 J. Nutr., October 1, 2004; 134(10): 2523 - 2527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang and P. J. Jones Dietary conjugated linoleic acid and body composition Am. J. Clinical Nutrition, June 1, 2004; 79(6): 1153S - 1158S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. AbuGhazaleh, D. J. Schingoethe, A. R. Hippen, and K. F. Kalscheur Conjugated Linoleic Acid Increases in Milk When Cows Fed Fish Meal and Extruded Soybeans for an Extended Period of Time J Dairy Sci, June 1, 2004; 87(6): 1758 - 1766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Moore, H. C. Hafliger III, O. B. Mendivil, S. R. Sanders, D. E. Bauman, and L. H. Baumgard Increasing Amounts of Conjugated Linoleic Acid (CLA) Progressively Reduces Milk Fat Synthesis Immediately Postpartum J Dairy Sci, June 1, 2004; 87(6): 1886 - 1895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Lin, J. J. Loor, and J. H. Herbein Trans10,cis12-18:2 Is a More Potent Inhibitor of De Novo Fatty Acid Synthesis and Desaturation than cis9,trans11-18:2 in the Mammary Gland of Lactating Mice J. Nutr., June 1, 2004; 134(6): 1362 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Brandsch, N. Nass, and K. Eder A Thermally Oxidized Dietary Oil Does Not Lower the Activities of Lipogenic Enzymes in Mammary Glands of Lactating Rats but Reduces the Milk Triglyceride Concentration J. Nutr., March 1, 2004; 134(3): 631 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Selberg, A. C. Lowe, C. R. Staples, N. D. Luchini, and L. Badinga Production and Metabolic Responses of Periparturient Holstein Cows to Dietary Conjugated Linoleic Acid and trans-Octadecenoic Acids J Dairy Sci, January 1, 2004; 87(1): 158 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Larsen, S. Toubro, and A. Astrup Efficacy and safety of dietary supplements containing CLA for the treatment of obesity: evidence from animal and human studies J. Lipid Res., December 1, 2003; 44(12): 2234 - 2241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. AbuGhazaleh, D. J. Schingoethe, A. R. Hippen, and K. F. Kalscheur Conjugated Linoleic Acid and Vaccenic Acid in Rumen, Plasma, and Milk of Cows Fed Fish Oil and Fats Differing in Saturation of 18 Carbon Fatty Acids J Dairy Sci, November 1, 2003; 86(11): 3648 - 3660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bernal-Santos, J. W. Perfield II, D. M. Barbano, D. E. Bauman, and T. R. Overton Production Responses of Dairy Cows to Dietary Supplementation with Conjugated Linoleic Acid (CLA) During the Transition Period and Early Lactation J Dairy Sci, October 1, 2003; 86(10): 3218 - 3228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Schroeder, J. E. Delahoy, I. Vidaurreta, F. Bargo, G. A. Gagliostro, and L. D. Muller Milk Fatty Acid Composition of Cows Fed a Total Mixed Ration or Pasture Plus Concentrates Replacing Corn with Fat J Dairy Sci, October 1, 2003; 86(10): 3237 - 3248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Peterson, E. A. Matitashvili, and D. E. Bauman Diet-Induced Milk Fat Depression in Dairy Cows Results in Increased trans-10, cis-12 CLA in Milk Fat and Coordinate Suppression of mRNA Abundance for Mammary Enzymes Involved in Milk Fat Synthesis J. Nutr., October 1, 2003; 133(10): 3098 - 3102. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
