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Journal of Dairy Science Vol. 57 No. 10 1258-1259
© 1974 by American Dairy Science Association ®
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Recovery of Residual Organic Matter from In Vitro Digestion of Forages1

J. E. Moore and G. O. Mott

Departments of Animal Science and Agronomy, University of Florida, Gainesville 32611

ABSTRACT

After two-stage in vitro digestion of about .44 g of initial forage organic matter, residues in fermentation tubes were filtered sequentially through glass wool mats, sintered glass crucibles, and glass fiber discs. Only .002 g of residual organic matter which passed glass wool was retained by sintered glass. The residue on sintered glass did not include recognizab'e forage particles. Glass fiber retained .029 g of residual organic matter which had passed both glass wool and sintered glass. Calculations of in vitro organic matter digestion were not affected by including the residue retained by sintered glass in addition to that retained by glass wool. Inclusion of the residue retained by glass fiber reduced digestion percentages by 1.9 units. Glass wool mats are easily prepared in 25-ml porcelain Gooch crucibles, and filtration through them is rapid.


FOOTNOTES

1 Florida Agricultural Experiment Stations Journal Series No. 5376.




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