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Journal of Dairy Science Vol. 44 No. 9 1768-1771
© 1961 by American Dairy Science Association ®
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Volatile Fatty Acid Analyses of Blood and Rumen Fluid by Gas Chromatography

E. S. Erwin, G. J. Marco and E. M. Emery

Monsanto Chemical Company St. Louis, Missouri

ABSTRACT

Short-chain fatty acids that originate from fermentation by rumen microflora are absorbed through the rumen wall and provide the principal source of energy for the ruminant animal. Furthermore, the relative concentrations of these fatty acids have been related to efficient energy utilization (2) and to milk fat production (5) in dairy cattle. Consequently, the development of an accurate, rapid method of short-chain acid analyses in blood and rumen fluid is of primary importance in ruminant nutrition. The recovery of radioactive acetate and butyrate was used in developing a suitable method for the preparation of rumen fluid and blood. Direct analyses of the resulting supernatant for short-chain acids were performed by gas chromatography.

In preliminary studies, rumen fluid, in which microbial activity had been destroyed, was acidified and extracted with diethyl ether for 20 hr. Initially, sodium methylate was added to the receiving flash containing ether and, following extraction, the sodium salts of fatty acids were acidified and diluted to volume.




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