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Journal of Dairy Science Vol. 36 No. 3 205-217
© 1953 by American Dairy Science Association ®
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Cultural Methods and Some Characteristics of Some of the More Numerous Groups of Bacteria in the Bovine Rumen

M. P. Bryant and L. A. Burkey

Bureau of Dairy Industry, Agricultural Research Administration, U. S. Department of Agriculture

ABSTRACT

It has been shown that the rumen of cattle contains billions of bacteria per milliliter of ingesta and that this bacterial flora brings about a complex fermentation process which is essential to the maintenance of normal digestion and nutrition of the animal.

Although large numbers of bacteria of diverse morphological characteristics can be demonstrated readily in rumen contents by microscopic examination, only a small number of these bacteria have been grown in pure culture and described as to their morphological and physiological characteristics. It has been recognized that many of the bacteria in the rumen cannot be cultivated by the more common bacteriological techniques (9) because of their apparently peculiar growth requirements. One of the most serious problems limiting the progress in the study of these bacteria has been the lack of development of adequate cultural methods.

In recent years methods have been devised with which large numbers of bacteria have been grown from rumen contents.




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