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Periprandial Changes in Metabolite and Metabolic Hormone Concentrations in High-Genetic-Merit Dairy Heifers and Their Relationship to Energy Balance in Early Lactation

A. R. G. Wylie^*,,1, S. Woods^,, A. F. Carson^, and M. McCoy^,#

^* Agri-Food and Biosciences Institute, Newforge Lane, Belfast BT9 5PX, United Kingdom
Queen’s University of Belfast, Newforge Lane, Belfast BT9 5PX, United Kingdom
College of Agriculture, Food and Rural Enterprise (CAFRE), 22 Greenmount Road, Antrim BT41 4PU, United Kingdom
Agri-Food and Biosciences Institute, Large Park, Hillsborough BT26 6DR, United Kingdom
^# The Department of Agriculture and Rural Development (DARD) Veterinary Service, Belfast BT4 3SF, United Kingdom

¹ Corresponding author: alastair.wylie{at}afbini.gov.uk

	ABSTRACT

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS ACKNOWLEDGEMENTS REFERENCES

 
Sixteen high-genetic-merit Holstein-Friesian heifers were offered a complete diet of grass silage, maize silage, and concentrates ad libitum through mo 1 to 3 postpartum. Open-circuit calorimetry and a 6-d digestibility balance were performed on each heifer at the end of each month, and energy balance (EB) was calculated. After each digestibility balance, heifers were blood sampled hourly from 1 h before to 9 h after feeding. Prefeed plasma and 3-h composites of postfeed plasmas were analyzed for selected metabolites and hormones. Levels of nonesterified fatty acids (NEFA) decreased, whereas those of β-hydroxybutyrate (BHBA) increased after feeding in each month. Urea levels increased after feeding, whereas glucose levels decreased in each month. Insulin increased after feeding but with reducing significance as lactation progressed. Insulin was always lower before feeding, and mean insulin increased from mo 1 to mo 3. Levels of insulin-like growth factor-1 (IGF-I) increased across mo 1 to 3, but were unaffected by feeding except in mo 2, whereas leptin levels varied significantly on each sampling occasion, but showed no increase between mo 1 and mo 3. Multiple regression of all data showed no significant correlation between EB and either BHBA or NEFA levels. However, EB was negatively correlated with leptin levels (r = –0.632), which were themselves positively associated with IGF-I (r = 0.498) and glucose (r = 0.565). A relationship was established between overall mean EB and prefeed leptin, insulin, and urea (R² = 0.63) in the first 3 mo of lactation. A potentially useful relationship was also established between EB and prefeed concentrations of leptin, IGF-I, urea, and glucose (R² = 0.80) only in mo 1 of lactation.

Key Words: energy balance • metabolic hormone • lactation • metabolite

	INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS ACKNOWLEDGEMENTS REFERENCES

 
Energy balance (EB) is a key concern in modern dairy herd management and represents the difference, on a daily basis, between the energy consumed by a cow and the energy expended in milk output and all supporting functions (locomotion, eating, digestion, and metabolism). Energy balance is strongly positive until a few weeks before calving but decreases with reducing feed intake in very late pregnancy. After calving, EB becomes negative (NEB) as the energy cost of milk production is factored in. The level of milk output from modern, high-genetic-merit (HGM) dairy cows means that EB is invariably negative and sometimes strongly so, well into lactation. Doepel et al. (2002) and Ferris et al. (2002b) identified the most negative EB (the EB nadir) within the first 2 wk postpartum in HGM cows, but NEB persisted for several months.

The significance of NEB for dairy farmers lies in the putative link between EB and cow fertility, which declined by almost 1% annually between 1975 and 1998 in the United Kingdom (Royal et al., 2000), threatening the economic viability and sustainability of many herds (Esslemont et al., 2001). Beam and Butler (1999) proposed that the depth or duration of the NEB, or the timing of the EB nadir affect the interval to resumption of reproductive activity in individual cows, through effects on follicular development (Lucy et al., 1992a,b; Beam and Butler, 1997), but supporting evidence for this has not always been forthcoming (Spicer et al., 1990; Snijders et al., 2001; Reist et al., 2003b). A reliable marker of EB might help identify the earliest realistic opportunity for insemination, reducing the costs of wasted semen and enhancing cow welfare.

Changes in BW and BCS have long been used by farmers as an intuitive indicator of EB but are insufficiently sensitive to pinpoint the EB nadir in a postpartum cow. Blood concentrations of NEFA and BHBA (a product of liver NEFA metabolism) have been used to suggest or confirm nutritional adequacy or inadequacy in farm animals since the 1960s (Ingvartsen et al., 2003) but the value of single and multiple indicators, such as those in the Compton metabolic profile (Payne et al., 1970), has been questioned (Adams et al., 1978; Rowlands, 1980). Blood levels of 3 functionally related metabolic hormones (insulin, IGF-I, and leptin) also change with nutritional status and one or more of these may have potential as markers of EB (Wathes et al., 2007a). There has been much interest in using leptin alone to indicate the EB nadir and predict the time of the first postpartum ovulation. Kadokawa et al. (2000) found a link between the interval from parturition to the leptin nadir and the time to first postpartum ovulation, with a delay in leptin recovery postpartum increasing time to first ovulation, but Liefers et al. (2003) could not identify a clear leptin nadir in a study of over 300 primiparous Holstein cows up to 80 d postpartum.

The current study set out to investigate if a reliable prediction of EB can be made in early lactation in dairy cows using concentrations of a small number of nutritionally dependent metabolites and hormones. Energy metabolism was assessed over 48 h in each of 16 first-lactation Holstein-Friesian cows at the end of mo 1 to 3 of lactation and used with milk yield (MY) and food intake data to calculate EB. Cows were then blood sampled from 1 h before to 9 h after feeding for analysis of NEFA, BHBA, urea, glucose, insulin, IGF-I, and leptin, and correlations were then sought between the determined EB and concentrations of these metabolites and hormones.

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS ACKNOWLEDGEMENTS REFERENCES

 
Animal Management and Measurements
Sixteen HGM autumn-calving heifers, with a PTA₂₀₀₅ (PTA using 2005 as base year) for fat plus protein of 21.7 ± 7.47 kg (mean ± SD), were used. Artificial insemination commenced at 14 mo (>315 kg), and heifers calved between September and January at 24.5 ± 1.31 mo and were then moved into individual cubicles. Feed intake and MY were recorded daily and BW and BCS weekly (scale of 1 to 5). Milk fat, protein, and lactose were determined every 2 wk on 3-d milk composites using a Milkoscan 605 (Foss Electric, Hillerød, Denmark). The study was approved by the AFBI (Hillsborough) Ethical Review Committee.

Diets and Diet Measurements
After calving, animals had ad libitum access to a complete diet of grass silage/maize (Zea mays L. cv. Loft) silage (60:40 DM basis) and concentrates (barley, wheat, molassed sugar beet pulp, citrus pulp, maize gluten, soybean meal, and rapeseed meal supplemented with minerals and vitamins). Concentrates were introduced at 4.0 kg/d and increased to 9.5 kg/d by d 20 of lactation. A Calan gate feeding system (American Calan, Northwood, NH) linked to Griffith-Elder weighing platforms (Griffith Elder, Bury St. Edmunds, UK) recorded intake at every meal. The amount of feed offered was adjusted to ensure approximately 10% orts. In mo 1 to 3 postpartum, feed was given once daily about 1030 h. In mo 7 only, cows grazed a grass pasture (except on the day of blood sampling, when harvested grass was fed). The silage was sampled daily and DM content was determined by drying overnight at 85°C. Dried samples were bulked weekly, milled, and analyzed for ADF, NDF, and ash (Cushnahan and Gordon, 1995). Fresh silage was analyzed weekly for volatiles-corrected oven DM, pH, nitrogen, VFA, gross energy, alcohols, and ammonia (Porter and Murray, 2001). Concentrate samples were bulked weekly and analyzed for DM, nitrogen, ash, ADF, NDF, and gross energy (Cushnahan and Gordon, 1995).

Energy Metabolism and Diet Digestibility Studies
At the end of mo 1, 2, and 3 postpartum, each animal was adapted to a tie-stall for 4 d and then transferred to an open-circuit respiration chamber for 3 d. Chamber operation was described by Gordon et al. (1995) with calibration according to Yan et al. (2000). Gas exchange and heat production data were collected over the last 48 h. After removal from the chamber, a 6-d digestibility balance (Mayne and Gordon, 1984) was performed on each cow.

Blood Sampling and Analysis
After each digestibility balance, a sterile jugular catheter was inserted (between 0800 and 0900 h). Patency was maintained by injecting 3 mL of a 50 IU/ mL solution of sodium heparin (CP Pharmaceuticals, Wrexham, UK) in sterile physiological saline (Baxter, Northants, UK). Blood was sampled into 20-mL poly-ethylene syringes 0.5 to 1 h before feeding (approximately 1000 h) and then hourly from 1 h to 9 h after feeding. Samples were immediately divided between plain glass tubes (for analysis of hormones), tubes containing fluoride oxalate (for analysis of glucose), and heparinized tubes (for analysis of metabolites). Serum and plasma were recovered by centrifugation and kept at –20°C until analyzed. Prefeed sera and plasma samples and three 3-h postfeed composites, comprising equal volumes of serum or plasma (taken at 1 to 3, 4 to 6, and 7 to 9 h), were analyzed for each animal. The postfeeding composites were prepared from once-thawed sera and plasmas.

Metabolite and Hormone Analyses
Metabolites were assayed as described by Keady et al. (1998) using auto-analyzer kits for NEFA (WaKo Chemicals, Neuss, Germany), urea, and BHBA (Randox, Antrim, UK). Hormones were determined by RIA. Insulin was assayed in all hourly samples, IGF-I in the prefeed sample and postfeed composites in mo 1, 2, 3, and 7, and leptin in the prefeed sample and postfeed composites in mo 1, 2, and 3 only (due to limited availability of radiolabeled leptin).

Insulin was determined using guinea pig anti-bovine insulin (Sigma, Poole, UK) at a final dilution of 1:80,000 bovine pancreatic insulin (Sigma) as assay standard (6.25 to 0.049 ng/mL) and ¹²⁵I-3-iodotyrosyl recombinant human insulin (Amersham, Bucks., UK) as label. Second antibody was SacCel donkey anti-guinea pig IgG (IDS, Washington, UK). Before IGF-I assay, binding proteins were removed by overnight extraction with ethanol:2 M HCl (7:1). An International Reference Standard recombinant human IGF-I (NIBSC, Potters Bar, UK) was used to construct the standard curve (3.0 to 0.0156 ng/tube). Primary antibody (UB3-189) was rabbit anti-human IGF-I (a gift from A. F. Parlow of the US National Hormone and Pituitary Program, Torrance, CA) used at a final dilution of 1:18,000, and ¹²⁵I-IGF-I (Amersham, UK) was used as label. This assay used goat anti-rabbit IgG (Sigma, UK) and polyethylene glycol to precipitate bound label. For the leptin assay, a standard curve was prepared using purified recombinant bovine leptin (Diagnostic Systems Laboratories, Oxford, UK), with a concentration range of 50 to 0.049 ng/mL. Pooled, previously characterized bovine serum was included at regular intervals in each assay for quality assurance purposes. The primary antibody (GP-OL3) was raised in guinea pigs against recombinant ovine leptin (reOL) generously donated by A. Gertler (Hebrew University of Jerusalem, Israel) and used at 1:160,000 final dilution. The second antibody was donkey anti-guinea pig IgG (IDS). ¹²⁵I-Recombinant ovine leptin was prepared by iodination of this same reOL with ¹²⁵I-sodium iodide (Amersham) using iodogen-coated tubes (Perbio Science, Northumberland, UK). Iodinated leptin was separated from unreacted iodine by gel filtration on G-75 Sephadex (GE Healthcare, Bucks, UK).

Statistical Analysis
Repeated-measures ANOVA using Genstat v.6 (Lawes Agricultural Trust, 2002) was used to allow for the successive time periods postcalving. To predict EB across the first 3 mo of lactation, forward stepwise regressions were run to generate multiple regression equations from mean concentration data for each metabolite and hormone and BCS, milk yield, feed intake, and BW data. To establish if sampling pre- or postprandially affected the predictability of EB, the regressions were carried out first using the prefeed data alone, then using postfeed data alone, and finally, using all of the data collected across the first 3 mo of lactation. All data were next reanalyzed by stepwise regression using forward selection of independent variables to establish relationships between all parameters, using probability levels of 5%. Regressions were calculated using total 3-mo data and then separately for data from each individual month.

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS ACKNOWLEDGEMENTS REFERENCES

 
Diet Characterization and Animal Performance Data
The grass silage had a DM content of 348 g/kg of fresh weight, an ME content of 12.5 MJ/kg DM, and a CP content of 158 g/kg of DM with a pH of 3.98. The maize silage had a DM content of 243 g/kg of fresh weight and a CP content of 81 g/kg of DM with a pH of 3.86. The grass and maize silages contained 292 and 311 g of ADF/kg of DM and 533 and 587 g of NDF/kg of DM, respectively, and ash contents were 89 and 57 g/kg of DM. The concentrates contained 218, 95, and 194 g/kg of DM of CP, ADF, and NDF, respectively, and had a gross energy of 17.8 MJ/kg DM. Mean and range values for EB, percentage ME retained, BW, BCS, MY, and feed intake are presented in Table 1.

Plasma Metabolite Concentrations
Table 2 shows the mean concentrations of each metabolite at each time point. Concentrations of NEFA decreased (P < 0.001) after feeding in mo 1, 2, and 3 but did not differ between the 3 postprandial composites in any of these months and were unaffected by feeding in mo 7. Concentrations of BHBA varied significantly (P < 0.001) across the 10-h sampling period in each of mo 1, 2, and 3 and also in mo 7 (P < 0.01). The BHBA levels were consistently lower before feeding than in any postprandial composite but this was significant in mo 1 to 3 only and there were no significant differences in mean BHBA between any of the postprandial composites in any month. In mo 7, BHBA concentrations increased steadily across the sampling period.

Serum Hormone Concentrations
Insulin levels increased significantly after feeding in each of mo 1 (P < 0.001), mo 2 (P < 0.01), and mo 3 (P < 0.05) but not in mo 7, and there was no significant difference between the hourly postprandial concentrations (Figure 1) or the 3-h postprandial composites (Table 3) in any of these months. In each of mo 1, 2, and 3, the greatest insulin concentration occurred between 4 and 5 h after feed was replenished. Insulin concentrations differed significantly (P < 0.05) in samples taken at 2, 3, and 4 h after feeding in mo 1 and 3 (Figure 1). Serum leptin concentrations (Table 3) were initially unaffected by feeding in mo 1, increasing only by 6 to 9 h after feeding (P < 0.001), whereas in mo 2, leptin levels increased immediately after feeding (P < 0.05) before quickly returning to prefeed levels. In mo 3, leptin concentrations increased some 4 to 6 h postprandially (P < 0.01) before returning to prefeed values. There was no significant difference between monthly mean leptin concentrations. Concentrations of IGF-I were unaffected by feeding in any month except mo 2 (Table 3), in which levels at 6 to 9 h after feeding were significantly greater than those immediately after feeding (P < 0.05). Overall mean IGF-I (pre- and postprandial) increased steadily but not significantly across the first 3 mo of lactation.

View larger version (15K): [in this window] [in a new window]   Figure 1. Periprandial changes in insulin concentrations at the end of mo 1, 2, 3, and 7 postcalving (n = 16). PF = prefeed; bars = SEM.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS ACKNOWLEDGEMENTS REFERENCES

 
The mean EB nadir value of –60.5 MJ/d in the current work was close to the –63.9 MJ/d estimated by Sutter and Beever (2000) for HGM Holstein-Friesian cows.

As anticipated, blood concentrations of all of the selected metabolites and hormones (except IGF-I) were significantly affected by feeding in each of the first 3 mo postpartum, with postprandial increases in BHBA, urea, insulin, and leptin and decreases in NEFA and glucose. In most instances where metabolite levels increased, they remained elevated throughout the 9-h postprandial sampling period. Urea, BHBA, and insulin showed the most immediate and proportionately greatest increases. In contrast, NEFA and glucose concentrations decreased sharply after feeding, whereas glucose levels recovered to preprandial values by the end of each sampling period. The contrast between pre- and postprandial metabolite concentrations highlights a longstanding concern about reliance on a single blood sample of variable and uncertain timing to provide information on an animal’s metabolic status.

Elevated blood BHBA levels are commonly used to confirm nutritional inadequacy in farm animals, and because BHBA is itself a product of NEFA metabolism by ruminant liver (Zammit, 1999), unusually high BHBA values (>1.5 mM) are taken to indicate low energy status and associated fat mobilization in cattle and sheep. But BHBA is also a product of butyrate metabolism by rumen epithelium and liver, which convert 75 and 60%, respectively, of butyrate passing through them to BHBA (Pennington, 1952). Rumen butyrate is increased in silage-fed ruminants offered barley-based supplements (Wylie et al., 1984), so high blood BHBA levels may not exclusively reflect mobilization of adipose reserves. β-Hydroxybutyrate is also a substrate for de novo fatty acid synthesis in the lactating bovine mammary gland (Vernon, 2005) so that elevated BHBA levels can make a positive contribution to lactation. In general, undernutrition is associated with a decrease in EB, suggesting an inverse correlation between EB and BHBA concentrations. In the current study, a similar postprandial BHBA increase was seen in each of mo 1 to 3 postpartum despite a very different EB in each. Conversely, Ferris et al. (2002b) saw no BHBA change in the first 52 d postpartum in HGM cows despite large EB changes and similarly concluded that BHBA levels are more closely related to diet type than to EB in early lactation. The sharp postprandial decrease in NEFA was greatest (65%) in mo 1. Because cows had no access to feed from the start of milking (0500 h) until 1030 h, and because Ferris et al. (2002a) noted that food intake was lowest between 2400 h and 0900 h in cows, then cows in the current study may have eaten little in the 10 or 11 h before feed renewal, promoting the very high prefeeding NEFA concentrations.

Plasma urea levels rose immediately after feeding in mo 1, 2, and 3 postpartum when the diet was a mixture of silage and concentrates. In mo 7, when fresh grass was given, an increase in plasma urea also occurred but was delayed until more than 3 h after feeding. In ruminants, high blood urea levels can follow an increase in protein intake, a change in protein quality, or a less synchronous supply of nitrogen and energy that limits the amount of ammonia assimilated into ruminal microbial protein (Sinclair et al., 1995). The small increase in plasma urea in the current study suggests that dietary protein was efficiently utilized despite the high protein level in the diet. The significant decrease in plasma glucose immediately after feeding in each month may have been linked to the near-doubling of insulin soon after feeding. In early lactation, insulin resistance in muscle (Bauman and Vernon, 1993) and adipose tissue (Etherton and Bauman, 1998) means that more glucose is partitioned to the mammary gland for lactose and milk fat synthesis, and the rapid fall in glucose in the current study may simply reflect a high glucose use for milk production in these HGM cows.

The incremental increase in insulin concentrations between mo 1 and mo 3 in the current study was similarly seen in postpartum cows by Ingvartsen and Andersen (2000) and in a shorter study by Spicer et al. (2002). Insulinogenic diets have been advocated in early lactation to encourage an early resumption of ovulation and help slow or reverse the decline in fertility in HGM cows with high levels of Holstein genetics (Gong, 2002). In general, in ruminants and nonruminants, a high plane of nutrition sustains insulin secretion and, through insulin, the activity of the hepatic growth hormone receptor (GHR) such that GH-induced IGF-I levels in blood are maximized (Breier et al., 1988). In dairy cows, however, expression of liver-specific GHR1A (the higher-affinity GHR isoform) is depressed at calving and recovers to precalving values only after 10 or more days (Kim et al., 2004). Thus, IGF-I levels decline by as much as 70% in the last few weeks prepartum (Block et al., 2001) but begin to rise again in well-fed cows from about 2 to 3 wk postpartum (Spicer et al., 1990; Doepel et al., 2002). Levels of IGF-I in the current study increased linearly across mo 1, 2, and 3 in line with increasing feed intake and insulin, but there was no further increase in IGF-I in mo 7 when cows were at grass and insulin levels were at their lowest. Taken collectively, studies by Beam and Butler (1999), Gutierrez et al. (1999), and Lucy et al. (1999) point to the insulin-GHR interaction that promotes IGF-I levels as a strong influence on ovarian follicular development. Disruption of this interaction by or during the NEB of early lactation (Fenwick et al., 2008) impedes resumption of ovulation and extends the calving interval. However, Gong et al. (2002) reported that high-insulin diets do not affect circulating IGF-I levels and argued that it is insulin’s direct effect on the differentiation and maturation of dominant follicles that increases the chance of ovulation in response to an LH surge.

Leptin concentrations varied significantly across the sampling day in each of mo 1 to 3 but with no consistent pattern, and mean leptin levels did not differ between mo 1 and 3. Leptin secretion in cattle is pulsatile (Ingvartsen and Boisclair, 2001; Wylie et al., 2001) but this was not considered to be of any practical significance in the current work in which postprandial leptin was assayed in 3-h composite samples. The earlier and proportionately greater increase in IGF-I than in leptin across mo 1, 2, and 3 paralleled the results of Meikle et al. (2004) in cattle, and those of Wylie et al. (2002a) in progressively refed fasted sheep, and is consistent with the prioritization of nutrient use for the restoration of muscle mass before fat mass as nutrition increases again above maintenance in previously underfed animals.

Of the metabolic hormones, only leptin was strongly correlated with mean EB through the first 3 mo of lactation (r = –0.632; P < 0.01). Reist et al. (2002) found only a weak association between EB and leptin (r = –0.027; P = 0.437) in wk 1 to 10 postpartum in HGM cows, whereas in a subsequent study (Reist et al., 2003b), they found no relationship between leptin and reproductive performance. Reist et al. (2003a) proposed that leptin is important in regulating feed intake, body fat stores, and energy homeostasis in transitional cows. An apparent lack of consistency across studies in relationships between leptin, energy status, and fertility may reflect the complexity of the multifactorial regulation of leptin in cows in early lactation (reviewed by Chilliard et al., 2005), but there is an absolute consensus that the management of adipose tissue reserves across the reproductive cycle is hugely important in meeting the reproductive priorities in dairy cows (Friggens, 2003).

Leptin and IGF-I concentrations were positively correlated (r = 0.498; P < 0.05) in the current study as in that by Reist et al. (2003a). Leptin and insulin were poorly correlated (r = 0.137), but IGF-I levels tended to be positively related to those of insulin (r = 0.397). Houseknecht et al. (2000) noted a correlation between leptin mRNA and IGF-I mRNA in cows and a positive association of each with insulin suggesting a functional triumvirate between these 3 metabolic hormones. Leptin concentrations have been correlated, to varying degrees, with body fat mass and BCS in sheep (Delavaud et al., 2000, Wylie, 2004), heifers (Leon et al., 2004), and dairy cows in mid and late lactation (Ehrhardt et al., 2000; Wylie et al., 2002b) making leptin a potentially useful marker of EB in dairy cows as suggested by Block et al. (2001). Further, IGF-I concentrations have been positively associated with muscle growth in several farm species (Oddy and Owens, 1996; Lobley, 1998) and with backfat thickness in 8-wk-old pigs (Suzuki et al., 2004), but not in beef bulls (Davis and Simmen, 2000), where the correlation was negative.

Given the inadequacy of single metabolites as predictors of EB, forward stepwise regressions were initially conducted using all preprandial and postprandial metabolite and hormone concentrations. The best-fit equation (R² = 0.89; P < 0.001; Table 5) used both pre-prandial and postprandial metabolite and hormone concentrations, but sampling and analysis on such a scale is unrealistic. The next-best-fit equation used only pre-feed samples (R² = 0.87; P < 0.01; Table 5) but required accurate estimates of feed intake, making it also of no practical use. After omitting parameters that are not easily measured, the best-fit equation involved only pre-feed values for leptin, urea, and insulin (R² = 0.63; P < 0.01; Table 5). However, this equation links mean EB in mo 1 to 3 of lactation to analyte levels determined throughout mo 1 to 3 and is, by definition, of no use as an anticipatory predictor of EB. When the analytical data and determined EB were regressed within each month, a potentially useful prediction for EB was obtained in mo 1 only [Mo 1 EB = –109.6 – 18.2 leptin (ng/mL) + 36.5 glucose (mM) – 0.466 IGF-I (ng/mL) + 19.67 urea (mM); R² = 0.80; P < 0.001)]. Month 3 EB was also significantly fitted to metabolite and hormone data but the relationship was not as strong [Mo 3 EB = –42.4 – 76.0 NEFA (mEq/L) + 10.49 urea (mM); R² = 0.51; P < 0.05)]. There was no significant relationship between EB and any of the analytes in mo 2.

Wathes et al. (2007a) collated data for the same metabolites and hormones as in the current study over 500 individual lactations in primiparous and multiparous cows to test relationships with cow fertility characteristics. Blood samples were taken from each cow (about 2 h after morning feeding) in the last 2 wk before calving and then in wk 2 to 3, 4 to 5, and 7 to 8 postpartum. A longer calving-to-conception interval was associated with greater prepartum leptin and lower prepartum NEFA and urea levels in multiparous cows. In a separate paper, Wathes et al. (2007b) compared interrelationships between the same metabolites and hormones and MY and BCS and stressed the importance of inherent differences in the control of nutrient partitioning between primiparous and multiparous cows that are consistent with primiparous cows diverting a greater proportion of their nutrient supply to tissue growth.

From the current work in primiparous cows, the most appropriate and useful equation for deriving EB used only leptin, urea, and insulin concentrations before feeding, whereas the best overall equation, for mo 1 only, used leptin, glucose, IGF-I, and urea concentrations. With current laboratory-based technology, measurement of these substances is expensive and outweighs any potential benefit. However, insulin, urea, and leptin are either already determinable or potentially determinable by immunobiosensors. Furthermore, all 3 are present and determinable in milk. Given the rapid pace of developments in nanotechnology and specifically in immunobiosensors, real-time milk hormone and metabolite assays may not be far away and could enable more accurate prediction of EB by noninvasive sampling.

	CONCLUSIONS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS ACKNOWLEDGEMENTS REFERENCES

 
The present study failed to establish significant correlations between NEFA or BHBA concentrations and EB, reinforcing skepticism about the reliability of these particular metabolites as sole indicators of nutritional status in early lactating dairy cows. Furthermore, although most of the hormones and metabolites measured in the present study exhibited significant periprandial variation, regression analysis of the data suggested that the most useful and opportune time to blood-sample cows in early lactation for estimation of EB is before morning feeding. This is an encouraging observation because it eliminates potential problems arising from greater postprandial variation in hormone levels, especially due to differences in feed intake between animals and because sampling can be more easily done at morning milking. The predictive equations generated by the current study may be improved by data from further similar studies and, with continuing progress in nanotechnology and the drive toward development of rapid, automated biosensors for human metabolic monitoring, there is a likelihood of being able to more accurately monitor the metabolic status of dairy cows in a timely way. What will then be needed is further reliable animal data on which to develop necessarily robust prediction equations.

	ACKNOWLEDGEMENTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS ACKNOWLEDGEMENTS REFERENCES

 
The authors wish to thank the Trustees of the former Agricultural Research Institute of Northern Ireland (Hillsborough, County Down) for the use of the facilities in which the work was conducted. S. Woods was funded by a postgraduate studentship awarded by the Department of Agriculture and Rural Development (DARD) for Northern Ireland. We also thank DARD and "Agri-Search" for funding this research. Metabolite analyses were conducted by AFBI Veterinary Science Division and statistical advice and analysis was provided by AFBI Biometrics Branch.

Received for publication May 25, 2007. Accepted for publication October 11, 2007.

	REFERENCES

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS ACKNOWLEDGEMENTS REFERENCES

 


Adams, R. S., W. L. Stout, and D. C. Kradel. 1978. Use and limitations of profiles in assessing health of nutritional status of dairy herds. J. Dairy Sci. 61:1671–1679.[Abstract/Free Full Text]

Bauman, D. E., and R. G. Vernon. 1993. Effects of exogenous bovine somatotropin on lactation. Annu. Rev. Nutr. 13:437–461.[CrossRef][Medline]

Beam, S. W., and W. R. Butler. 1997. Energy balance and ovarian follicle development prior to the first ovulation postpartum in dairy cows receiving three levels of dietary fat. Biol. Reprod. 56:133–142.[Abstract]

Beam, S. W., and W. R. Butler. 1999. Effect of energy balance on follicular development and first ovulation in post partum dairy cows. J. Reprod. Fertil. 54(Suppl.):411–424.

Block, S. S., W. R. Butler, R. A. Ehrhardt, A. W. Bell, M. E. Van Amburgh, and Y. R. Boisclair. 2001. Decreased concentrations of plasma leptin in periparturient dairy cows is caused by negative energy balance. J. Endocrinol. 171:339–348.[Abstract]

Breier, B. H., P. D. Gluckman, and J. J. Bass. 1988. The somatotrophic axis in young steers: Influence of nutritional status and oestradiol-17β on hepatic high- and low-affinity somatotrophic binding sites. J. Endocrinol. 116:169–177.[Abstract/Free Full Text]

Chilliard, Y., C. Delavaud, and M. Bonnet. 2005. Leptin expression in ruminants: Nutritional and physiological regulations in relation with energy metabolism. Domest. Anim. Endocrinol. 29:3–22.[CrossRef][Medline]

Cushnahan, A., and F. J. Gordon. 1995. The effects of grass preservation on intake, apparent digestibility and rumen degradation characteristics. Anim. Sci. 60:429–438.

Davis, M. E., and R. C. M. Simmen. 2000. Genetic parameter estimates for serum insulin-like growth factor-1 concentration and carcass traits in Angus beef cattle. J. Anim. Sci. 78:2305–2313.[Abstract/Free Full Text]

Delavaud, C., F. Bocquier, Y. Chilliard, D. H. Keisler, A. Gertler, and G. B. Kann. 2000. Plasma leptin determination in ruminants: Effect of nutritional status and body fatness on plasma leptin concentration assessed by a specific RIA in sheep. J. Endocrinol. 165:519–526.[Abstract]

Doepel, L., H. Lapierre, and J. J. Kennelly. 2002. Peripartum performance and metabolism of dairy cows in response to prepartum energy and protein intake. J. Dairy Sci. 85:2315–2334.[Abstract/Free Full Text]

Ehrhardt, R. A., R. M. Slepetsis, J. Siegal-Willott, M. E. Van Am-burgh, A. W. Bell, and Y. R. Boisclair. 2000. Development of a specific radioimmunoassay to measure physiological changes of circulating leptin in cattle and sheep. J. Endocrinol. 166:519–528.[Abstract]

Esslemont, R. J., M. A. Kossaibati, and J. Allcock. 2001. Economics of fertility in dairy cows. Fertility in the High-Producing Dairy Cow. M. G. Diskin, ed. Occ. Pub. No. 26, Brit. Soc. Anim. Sci., Penicuik, Midlothian, UK.

Etherton, T. D., and D. E. Bauman. 1998. Biology of somatotropin in growth and lactation of domestic animals. Physiol. Rev. 78:745–761.[Abstract/Free Full Text]

Fenwick, M. A., R. Fitzpatrick, D. A. Kenny, M. G. Diskin, J. Patton, J. J. Murphy, and D. C. Wathes. 2008. Interrelationships between negative energy balance (NEB) and IGF regulation in liver of lactating dairy cows. Domest. Anim. Endocrinol. 34:31–44.[CrossRef][Medline]

Ferris, C. P., R. C. Binnie, J. P. Frost, and D. C. Patterson. 2002a. A comparison of two silage feeding systems, involving different inputs, for dairy cows. Pages 382–383 in Proc. XIIIth Int. Silage Conf. L. M. Gechie and C. Thomas, ed. Scottish Agric. Coll., Ayr, Scotland, UK.

Ferris, C. P., M. A. McCoy, S. D. Lennox, D. C. Catney, and F. J. Gordon. 2002b. Nutrient utilisation and energy balance associated with two contrasting winter milk producing systems for high genetic merit autumn calving dairy cows. Irish J. Agric. Food Res. 41:55–70.

Friggens, N. C. 2003. Body lipid reserves and the reproductive cycle: Towards a better understanding. Livest. Prod. Sci. 83:219–236.[CrossRef]

Gong, J. G. 2002. Influence of metabolic hormones and nutrition on ovarian follicle development in cattle: Practical implications. Domest. Anim. Endocrinol. 23:229–241.[CrossRef][Medline]

Gong, J. G., W. J. Lee, P. C. Garnsworthy, and R. Webb. 2002. Effect of dietary-induced increases in circulating insulin concentrations during the early postpartum period on reproductive function in dairy cows. Reproduction 123:419–427.[Abstract]

Gordon, F. J., M. G. Porter, C. S. Mayne, E. F. Unsworth, and D. J. Kilpatrick. 1995. The effect of forage digestibility and type of concentrate on nutrient utilisation for lactating dairy cattle. J. Dairy Res. 62:15–27.[Medline]

Gutierrez, C. G., J. G. Gong, T. A. Bramley, and R. Webb. 1999. Effects of genetic selection for milk yield on metabolic hormones and follicular development in postpartum dairy cattle. J. Reprod. Fertil. Abstr. Ser. 24:32. (Abstr.)

Houseknecht, K. L., C. P. Portocarrero, S. Lemenager, R. Ji, and M. E. Spurlock. 2000. Growth hormone regulates leptin gene expression in bovine adipose tissue: Correlation with adipose IGF-1 expression. J. Endocrinol. 164:51–57.[Abstract]

Ingvartsen, K. L., and J. B. Andersen. 2000. Integration of metabolism and intake regulation: A review focusing on periparturient animals. J. Dairy Sci. 83:1573–1597.[Abstract]

Ingvartsen, K. L., and Y. R. Boisclair. 2001. Leptin and the regulation of food intake, energy homeostasis and immunity with special focus on periparturient ruminants. Domest. Anim. Endocrinol. 21:215–250.[CrossRef][Medline]

Ingvartsen, K. L., R. J. Dewhurst, and N. C. Friggens. 2003. On the relationship between lactational performance and health: Is it yield or metabolic imbalance that cause production diseases in dairy cattle? A position paper. Livest. Prod. Sci. 83:277–308.[CrossRef]

Kadokawa, H., D. Blache, Y. Yamada, and G. B. Martin. 2000. Relationships between changes in plasma concentrations of leptin before and after parturition and the timing of first postpartum ovulation in high-producing Holstein dairy cows. Reprod. Fertil. Dev. 12:405–411.[CrossRef][Medline]

Keady, T. W. J., C. S. Mayne, and M. Marsden. 1998. The effects of concentrate energy source on silage intake and animal performance with lactating dairy cows offered a range of grass silages. Anim. Sci. 66:21–33.

Kim, J. W., R. P. Rhoads, S. S. Block, T. R. Overton, S. J. Frank, and Y. R. Boisclair. 2004. Dairy cows experience selective reduction of the hepatic growth hormone receptor during the periparturient period. J. Endocrinol. 181:281–290.[Abstract]

Lawes Agricultural Trust. 2002. Genstat. 6th ed. Lawes Agricultural Trust, Rothamsted, UK.

Leon, H. V., J. Hernandez-Ceron, D. H. Keisler, and C. G. Gutierrez. 2004. Plasma concentrations of leptin, insulin like growth factor-1, and insulin in relation to changes in body condition score in heifers. J. Anim. Sci. 82:445–451.[Abstract/Free Full Text]

Liefers, S. C., R. F. Veerkamp, M. F. W. te Pas, C. Delavaud, Y. Chilliard, and T. van der Lende. 2003. Leptin concentrations in relation to energy balance, milk yield, intake, live weight and oestrus in dairy cows. J. Dairy Sci. 86:799–807.[Abstract/Free Full Text]

Lobley, G. E. 1998. Nutritional and hormonal control of muscle and peripheral tissue metabolism in farm species. Livest. Prod. Sci. 56:91–114.[CrossRef]

Lucy, M. C., J. Beck, C. R. Staples, H. H. Head, R. L. Delasota, and W. W. Thatcher. 1992a. Follicular dynamics, plasma metabolites, hormones and insulin-like growth factor-1 (IGF-1) in lactating cows with positive or negative energy balance during the preovulatory period. Reprod. Nutr. Dev. 32:331–341.[CrossRef][Medline]

Lucy, M. C., C. R. Bilby, C. J. Kirby, W. Yuan, and C. K. Boyd. 1999. Role of growth hormone in development and maintenance of follicles and corpora lutea. J. Reprod. Fertil. 54(Suppl.):49–59.

Lucy, M. C., J. D. Savio, L. Badinga, R. L. Delasota, and W. W. Thatcher. 1992b. Factors that affect ovarian follicular dynamics in cattle. J. Anim. Sci. 70:3615–3626.[Abstract]

Mayne, C. S., and F. J. Gordon. 1984. The effect of type of concentrate and level of concentrate feeding on milk production. Anim. Prod. 39:65–76.

Meikle, A., M. Kulcsar, Y. Chilliard, H. Febel, C. Delavaud, D. Cavestany, and P. Chilibroste. 2004. Effects of parity and body condition at parturition on endocrine and reproductive parameters of the cow. Reproduction 127:727–737.[Abstract/Free Full Text]

Oddy, V. H., and P. C. Owens. 1996. Insulin-like growth factor 1 inhibits degradation and improves retention of protein in hind-limb muscle of lambs. Am. J. Physiol. Endocrinol. Metab. 34:E973–E982.

Payne, J. M., S. M. Dew, R. Manston, and M. Faulks. 1970. The use of a metabolic profile test in dairy herds. Vet. Rec. 87:150–158.[Medline]

Pennington, R. J. 1952. The metabolism of short-chain fatty acids in sheep. 1. Fatty acid utilisation and ketone body production by rumen epithelium and other tissues. Biochem. J. 51:251–258.[Medline]

Porter, M. G., and R. S. Murray. 2001. The volatility of components of grass silage on oven drying and the inter-relationship between dry matter content estimated by different analytical methods. Grass Forage Sci. 56:405–411.[CrossRef]

Reist, M., D. Erdin, D. von Euw, K. Tschuemperlin, H. Leuenberger, Y. Chilliard, H. M. Hammon, C. Morel, C. Philipona, Y. Zbinden, N. Kunzi, and J. W. Blum. 2002. Estimation of energy balance at the individual and herd level using blood and milk traits in high yielding dairy cows. J. Dairy Sci. 85:3314–3327.[Abstract/Free Full Text]

Reist, M., D. Erdin, D. von Euw, K. Tschumperlin, H. Leuenberger, C. Delavaud, Y. Chilliard, H. M. Hammon, N. Kunzi, and J. W. Blum. 2003a. Concentrate feeding strategy in lactating dairy cows: Metabolic and endocrine changes with emphasis on leptin. J. Dairy Sci. 86:1690–1706.[Abstract/Free Full Text]

Reist, M., D. K. Erdin, D. von Euw, K. M. Tschumperlin, H. Leuenberger, H. M. Hammon, C. Morel, C. Philipona, Y. Zbinden, N. Kunzi, and J. W. Blum. 2003b. Postpartum reproductive function: Association with energy, metabolic and endocrine status in high yielding dairy cows. Theriogenology 59:1707–1723.[CrossRef][Medline]

Rowlands, G. J. 1980. A review of variations in the concentrations of metabolites in the blood of beef and dairy cattle associated with physiology, nutrition and disease, with particular reference to the interpretation of metabolic profiles. World Rev. Nutr. Diet. 35:172–235.[Medline]

Royal, M. D., A. O. Darwash, A. P. F. Flint, R. Webb, J. A. Woolliams, and G. E. Lamming. 2000. Declining fertility in dairy cattle: Changes in traditional and endocrine parameters of fertility. Anim. Sci. 70:487–501.

Sinclair, L. A., P. C. Garnsworthy, J. R. Newbold, and P. J. Buttery. 1995. Effects of synchronizing the rate of dietary energy and nitrogen release in diets with a similar carbohydrate composition on rumen fermentation and microbial protein synthesis in sheep. J. Agric. Sci. 124:463–472.

Snijders, S. E. M., M. G. Dillon, K. J. O’Farrell, M. Diskin, A. R. G. Wylie, D. O’Callaghan, M. Rath, and M. P. Boland. 2001. Genetic merit for milk production and reproductive success in dairy cows. Anim. Reprod. Sci. 65:17–31.[CrossRef][Medline]

Spicer, L. J., C. C. Francisco, D. Jones, and D. N. Waldner. 2002. Changes in plasma insulin and glucose concentrations during early lactation in Holstein cows. Oklahoma State Univ. Anim. Sci. Res. Rept. (Article Number 33). Oklahoma State Univ., Stillwater.

Spicer, L. J., W. B. Tucker, and G. D. Adams. 1990. Insulin like growth factor-1 in dairy cows: Relationships among energy balance, body condition, ovarian activity and estrus behaviour. J. Dairy Sci. 73:938–947.[Abstract]

Sutter, F. D., and D. E. Beever. 2000. Energy and nitrogen metabolism in Holstein-Friesian cows during early lactation. Anim. Sci. 70:503–514.

Suzuki, K., M. Nakagawa, K. Katoh, H. Kadowaki, T. Shibata, H. Uchida, Y. Obara, and A. Nishida. 2004. Genetic correlation between serum insulin-like growth factor-1 concentration and performance and eating quality traits in Duroc pigs. J. Anim. Sci. 82:994–999.[Abstract/Free Full Text]

Vernon, R. G. 2005. Lipid metabolism during lactation: A review of adipose tissue-liver interactions and the development of fatty liver. J. Dairy Res. 72:460–469.[CrossRef][Medline]

Wathes, D. C., N. Bourne, Z. Cheng, G. E. Mann, V. J. Taylor, and M. P. Coffey. 2007a. Multiple correlation analyses of metabolic and endocrine profiles with fertility in primiparous and multiparous cows. J. Dairy Sci. 90:1310–1325.[Abstract/Free Full Text]

Wathes, D. C., A. Cheng, N. Bourne, V. J. Taylor, M. P. Coffey, and S. Brotherstone. 2007b. Differences between primiparous and multiparous dairy cows in the inter-relationships between metabolic traits, milk yield and body condition score in the periparturient period. Domest. Anim. Endocrinol. 33:203–225.[CrossRef][Medline]

Wylie, A. R. G. 2004. Association of plasma leptin concentrations with fat depot accumulation in growing sheep. Page 129 in Proc. Brit. Soc. Anim. Sci. Penicuik, Midlothian, UK.

Wylie, A. R. G., D. J. Devlin, and A. J. Bjourson. 2002a. Profiles of leptin and IGF-1 and inter-relationships between energy-related metabolites and hormones in fed and fasted sheep. Page 87 in Proc. Brit. Soc. Anim. Sci. Penicuik, Midlothian, UK.

Wylie, A. R. G., D. J. Devlin, T. W. J. Keady, C. S. Mayne, R. E. Agnew, and T. Yan. 2002b. Serum leptin concentrations are a poor predictor of body fat content in lactating multiparous dairy cows. Page 91 in Proc. Brit. Soc. Anim. Sci. Penicuik, Midlothian, UK.

Wylie, A. R. G., D. R. Mackey, D. J. Devlin, A. J. Bjourson, and M. G. Diskin. 2001. Plasma leptin in intact and ovariectomised beef heifers on low and high planes of nutrition. Page 43 in Proc. Agric. Res. Forum, Tullamore, Ireland. Teagasc, Dublin, Ireland.

Wylie, A. R. G., E. F. Unsworth, and R. Anderson. 1984. Volatile fatty acid proportions and production rates in the rumen of sheep offered wilted and direct-cut silages, with and without supplementary concentrates. Pages 31–32 in Proc. Vllth Silage Conf., Belfast, UK. Queen’s University of Belfast, Belfast, UK.

Yan, T., R. E. Agnew, F. J. Gordon, and M. G. Porter. 2000. The prediction of methane energy output in dairy and beef cattle offered grass silage-based diets. Livest. Prod. Sci. 64:253–263.[CrossRef][Medline]

Zammit, V. A. 1999. Ketogenesis in the liver of ruminants – Adaptations to a challenge. J. Agric. Sci. 115:155–162.

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