JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Interpretive Summary
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herzallah, S. M.
Right arrow Articles by Al-Ismail, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herzallah, S. M.
Right arrow Articles by Al-Ismail, K. M.
J. Dairy Sci. 88:1301-1310
© American Dairy Science Association, 2005.

Effect of Heating and Processing Methods of Milk and Dairy Products on Conjugated Linoleic Acid and Trans Fatty Acid Isomer Content

S. M. Herzallah1, M. A. Humeid2 and K. M. Al-Ismail2

1 Department of Nutrition and Food Technology, Faculty of Agriculture, University of Mu’tah, Jordan
2 Department of Nutrition and Food Technology, Faculty of Agriculture, University of Jordan, Jordan

Corresponding author: S. M. Herzallah; e-mail: saqermay{at}yahoo.com.


   ABSTRACT
TOP
 ABSTRACT
INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 CONCLUSIONS
 REFERENCES
 
The conventional heating methods of milk did not cause any significant increase in the trans isomer content, with the exception of milk heated at 63 ± 1.0°C for 30 min and milk microwaved for 5 min, which were significantly increased by 19 and 31%, respectively. The chemical changes of lipids were generally accelerated with the severity of the heat treatment and duration of storage. The conjugated linoleic acid content of cheese heated in a microwave oven for 5 min decreased by 21%, and microwave heating for 10 min caused a decrease of 53% compared with that of freshly boiled cheese.

Key Words: Nabulsi cheese • microwave • conventional heating • conjugated linoleic acid

Abbreviation key: CLA = conjugated linoleic acid.


   INTRODUCTION
TOP
 ABSTRACT
 INTRODUCTION
MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 CONCLUSIONS
 REFERENCES
 
Milk lipids are considered one of the outstanding milk constituents with respect to presence of lipid classes, and variety and number of identified fatty acids, which was found to be more than 400 (Jensen et al., 1990, 1991). Milk lipids include anticarcinogenic compounds such as conjugated linoleic acid (CLA), sphingomyelin, and butyric acid (Ames et al., 1995; Parodi, 1999). Milk lipids may undergo chemical and physical changes during processing and storage such as auto-oxidation and formation of trans fatty acids (Semma, 2002).

Trans fatty acids are naturally found in low concentrations in dairy products because of the biohydrogenation process in the rumen but may also be formed during processing of dairy products at high temperatures such as in baking or frying. Trans fatty acids have been associated with biological and toxicological effects such as coronary heart disease, and disturbances of the metabolism of the essential fatty acids in the fetus, which could affect intrauterine human growth (Addis, 1990; Addis and Warner, 1991; Kumar and Singhal, 1991; Willett and Ascherio, 1994; Boué et al., 2000).

Milk and milk products usually undergo different changes during their preparation (boiling and microwaving) or processing, which may include moderate or severe heat treatments that can lead to undesirable changes in lipids or proteins. Microwave ovens are widely used for cooking and reheating of foods in millions of kitchens throughout the world. Food heating by microwave results from the conversion of microwave energy into heat by friction of vibrating water molecules due to rapid fluctuations in the electromagnetic field (Decareau, 1992; Potter and Hotchkiss, 1996).

The trend of using microwave ovens in food preparation is attributed to the speed of heating and energy saving. Although microwave ovens are widely used as a means of food preparation, insufficient information is available on the consequences for the composition and nutritional quality of food. Some studies revealed that microwave heating affected fat oxidation and fatty acid isomer formations (Albi et al., 1997a,b).

Variations in heating treatments may have different effects on CLA and trans isomers. Therefore, the objective of this study was to evaluate the effect of heating treatments (microwave and conventional heating), processing steps of milk and milk products [yogurt, labaneh, brined white cheese (Nabulsi), and UHT], and storage conditions on CLA and trans fatty acid isomer contents.


   MATERIALS AND METHODS
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
RESULTS AND DISCUSSION
 CONCLUSIONS
 REFERENCES
 
Raw cows’ milk used in the study was obtained from the bulk tank of 3 dairy plants: Danish Jordan Dairy Company, Jordan University Dairy Plant, and Al-Sanabel Dairy Co. The milks were produced by the Cows’ Breeder Society, the Jordan University farm, and the Haj Mustafa farm, respectively. Brined white cheese (Nabulsi) was produced from ewes’ milk provided by Al-Sanabel Dairy Co.; yogurt and labaneh were from Danish Jordan Dairy Company and Jordan University Dairy Plant, respectively. The UHT (at 140 ± 1.0°C) and pasteurized (at 85.0 ± 1.0°C) milk were from Danish Jordan Dairy Company.

Heat Treatments of Milk and Milk Products
The raw cows’ milk obtained from the 3 sources was subjected to different heat treatments as shown in Table 1Go.


View this table:
[in this window]
[in a new window]
  		Table 1. Cows’ milk and milk products produced by different heat treatments.
 
Production of Set Yogurt
Set yogurt was produced from milk pasteurized at 95 ± 1.0°C for 5 min (tube pasteurization), 95 ± 1.0°C for 15 min (plate pasteurization), or 85 to 90°C for 2 min (batch pasteurization). The milk was cooled to 45 ± 1.0°C and inoculated with 2 to 3% freeze-dried mixed starter culture (Danisco, Denmark). It was then poured into plastic containers of different sizes, and incubated at 42 ± 1.0°C for up to 2.5 h. When the desired acidity of 0.7% (pH 4.5 to 4.6) was reached, the yogurt was cooled to 4.0 ± 1.0°C.

Labaneh Production
Traditional method (cloth sack).
After cooling, the set yogurt was stirred and then poured into a cloth sack to drain off the whey for 12 to 24 h (overnight). The drained yogurt labaneh was salted with 1% NaCl, blended, poured into suitable plastic containers, and refrigerated at 4°C. The produced labaneh was 23 to 25% total soluble solids and had a pH of 5 to 5.5.

Separator method (centrifugal separator).
Cream was separated, milk was pasteurized at 83 to 85°C for 16 s, salt (1% NaCl) was added, the pasteurized skim milk was inoculated with ~0.002% (wt/vol) powdered, freeze-dried, mixed starter culture (Danisco) and kept for 15 to 17 h at 42 to 44°C. When the pH of the yogurt reached 4.5 to 4.6, the product was stirred and the whey separated via separator at 40°C. The concentrated skim yogurt and the cream (40% butter) were mixed to have total solids of not less than 23% and 10% fat. The produced labaneh was poured into suitable plastic containers and stored at 4°C.

Cheese Production and Heat Treatments
White brined cheese (Nabulsi) was produced according to the traditional method described by Humeid and Tukan (1986) and Humeid et al. (1990) from ewes’ milk. Two desalted, grated cheese samples of approximately 200 g each were heated in a microwave oven (800 W, WD800B, Galanz, Korea) at 80% power. The first sample was heated at 96.3 ± 1.0°C until browning (~10 min), and the second was placed in a polyethylene bag and then in a Pyrex saucepan filled with water (distilled water), and boiled while floating in the microwave oven at 96.3 ± 1.0°C for 5 min. Another 2 cheese samples (~100 g each) were desalted, grated, placed in polyethylene bags in a Pyrex saucepan, covered with distilled water, and boiled on a gas stove for 5 min at 95.5 ± 1.0°C.

UHT Reconstituted Milk
Ultra-high temperature milk reconstituted from powdered milk (Kuwaiti Danish Dairy Co., Kuwait) was purchased from the local market for comparison (production date 09/09/02 and expired on 09/03/03).

Storage of Milk and Milk Products
The milk and milk products used in the study (pasteurized milk, UHT milk, yogurt, and labaneh) were stored at 5.0 ± 1.0°C and analyzed after a storage period of 3, 5, 7, and 15 d for pasteurized milk, UHT milk, yogurt, and labaneh, respectively, as indicated on the package label (i.e., the commercial shelf life). The produced white brined cheese (Nabulsi) was evaluated after 1 mo of storage in tins at 18 ± 1.0°C.

Milk Fat Extraction and Analysis
Lipids were extracted from the milk and milk products using chloroform and methanol as described by Bligh and Dyer (1959) with some modifications regarding sample weight, solvent volume, and centrifugation speed and time. Approximately 70 g of cheese, yogurt, or labaneh, or 100 mL of fluid milk product was homogenized with 100 mL of methanol and 100 mL of chloroform using a Hamilton Beach Scovel homogenizer (NSF, Racine, WI) for 2 min at medium speed. Then, 100 mL of chloroform was added to the mixture, and homogenized for an additional 2 min. The homogenate was centrifuged at 4000 rpm for 20 min using an Heraeus centrifuge (Heraeus Christ, GmbH, Osterode/Harz, Germany). The upper layer (methanol and water) was removed through aspiration. The middle and the lower layers (chloroform and precipitated protein, respectively) were filtered through filter paper to separate precipitate particles. The chloroform-lipid extracts were again filtered through anhydrous sodium sulfate (Na2SO4) and the Na2SO4 was rinsed 3 times with 30 mL of chloroform (10 mL each rinse). The lipid extracts were dried under nitrogen using a rotoevaporator (Laborota, 4001 WB, Heidolph, Germany) at 150 rpm and 50°C, and stored in 5-mL brown glass vials under nitrogen at –18°C. The lipid samples were then used for the analysis of CLA and fatty acids trans isomers.

Chemical and Instrumental Analyses
Fourier transform infrared spectroscopy was used to determine trans isomer contents in lipid extracts. The American Oil Chemists Society official method Cd 14–61 was used for the determination of the trans content of fats (Walker, 1980; Gunstone, 1986). The transmittance of the isolated trans double bond was measured in the infrared region at a wavenumber of 967 cm–1, which is equivalent to a wavelength of 10.34 µm. The transmittant band of deformated C-H bond about the trans double bonds is typical of the isolated trans group. On the contrary, the cis and saturated fatty acids do not have such a spectrum. Therefore, measurement of such band intensity forms the basis for the trans isomers determination (Conacher, 1976; Svensson et al., 1982; Yamaoka et al., 1987; Mossoba et al., 1990; Ulberth and Haider, 1992; Mossoba et al., 1993; Toschi et al., 1993; Guillen and Cabo, 1997).

Esterification of Lipid Samples and Elaidic Acid
Two milliliters of elaidic acid (2 mg/mL) and 5 selected lipid extracts were accurately weighed (50 to 100 mg) and dissolved in toluene (1 mL). Two milliliters of 2% sulfuric acid (Fisher Scientific Co., Fairlawn, NJ) in methanol (Lab-Scan, Dublin, Ireland) was added. The mixture was incubated overnight in stoppered tubes at 50°C, then 5 mL of water containing 5% NaCl (GCC Laboratory, Clwyd, UK) was added and the esters were extracted 3 times with 3 mL of hexane (Lab-Scan) using Pasteur pipettes to remove each layer. The hexane layer was washed with 4 mL of 2% potassium carbonate (BDH Laboratory, Poole, UK). The solution was filtered through a filter paper (Ederol, 5.5 mm, Hatzfeld, Eder, Germany), and was then dried over 99.0% anhydrous sodium sulfate (SDS Fine Chemicals, Ltd., Mumbai, India). The solvent was removed under a stream of nitrogen gas (Christie, 1992). The dried extract was dissolved in carbon disulfide (GCC Laboratory) and trans isomers spectra were collected by a Fourier transform infrared spectrometer 670 (Thermo Nicolet Nexus, Thermo Electron Corp., at the Chemistry Department, Jordan University. A demountable liquid cell of 150 µL capacity, 0.015 mm thickness, and 25 mm diameter NaCl windows (Buck Scientific, Inc., Germany) was used for measurement of the trans isomers of the standard and the lipid extract.

Measurement of Trans Isomers
A calibration curve was made with elaidic acid (C17H33COOH standard; BDH Laboratory Supplies) in the form of methylelaidate. The standards were dissolved in carbon disulfide at concentrations of 0.04, 0.08, 0.2, 1.0, 2.0, and 5.0 mg/mL. The demountable cell of the spectrometer was filled carefully to avoid air bubble entrapment. The cell was washed 3 times with pure carbon disulfide and 3 times with the analyzed fatty acid methyl ester before each analysis. Thirty analytical scans were used; the range spectrum was obtained at wavenumbers between 800 and 1200 cm–1. Each sample was scanned 3 times in succession and scanned again after 10 min. The recovery test for methyl elaidate was conducted by spiking a starch sample (10 g) with 3 mL of methyl elaidate (20 mg/mL), followed by 4 replicate complete analyses. Then, the methyl ester was extracted, dried under nitrogen gas, dissolved in approximately 2 mL of CS2, transferred into a 25-mL volumetric flask, and the volume completed with CS2 (GCC Laboratory). The minimum detection limit of trans isomers as methyl elaidate was found to be 20 ppm. The calibration curve is shown in Figure 1Go and the averaged value of recoveries was 97.8% for methyl elaidate, as shown in Table 2Go.



View larger version (11K):
[in this window]
[in a new window]
  		Figure 1. Linearity of the results for methyl elaidate as measured by Fourier transform infrared spectroscopy.

 

View this table:
[in this window]
[in a new window]
  		Table 2. Recoveries and confidence range of methyl elaidate (addition of 60 µg).
 
CLA
Conjugated linoleic acid was determined by HPLC (Pump 600, 486 UV detector, manual injection Reodyne and 2010-Mellenium software, Waters Co., Milford, MA) using the chromatograph at the Water and Environmental Study and Research Center at Jordan University.

Derivatization of CLA from Lipid Extracts
A sample of 40 to 100 mg of the extracted lipids was weighed and derivatized in screw-capped tubes with 6 mL of 4% HCl (Frutarom, Kettering, UK) in HPLC-grade methanol (Lab-Scan) for 40 min at 60°C. The tubes were cooled to 18 ± 1°C and diluted with 2 mL of double distilled water. The CLA methyl ester was then extracted 3 times with 2 mL of HPLC-grade hexane (Lab-Scan). The hexane extract was washed twice with double distilled water and dried over anhydrous sodium sulfate (SDS Fine Chemicals). A 1-mL portion of the hexane extract was dried under nitrogen and redissolved in 1 mL of methanol for HPLC analysis of total CLA. The remaining portion of hexane was stored at –18°C (Chin et al., 1992).

Quantification of CLA in Lipid Extracts
Quantification of the total CLA methyl esters in fat was performed with a Waters HPLC system. This HPLC is equipped with solvent delivery system capable of pumping 4 different solvents that make a gradient solvent delivery system. The CLA methyl ester in methanol (20 µL) was injected into the HPLC column through the injection port. Separation was performed on a reversed phase analytical column (Sherisorb ODS2, 5 µm, 150 mm x 4.6 mm i.d., Waters). The gradient mobile phase was delivered at a flow rate of 0.5 mL/min as shown in Table 3Go. Eluents were monitored at 245 nm using a Waters 486 UV tunable detector, controller system 600, pump 600E, and Millennium software 2010 Chromatography Manager. Quantification of total CLA in a sample was based on the external standard calibration curve.


View this table:
[in this window]
[in a new window]
  		Table 3. High performance liquid chromatography conditions.
 
Preparation of CLA Methyl Ester Calibration Curve
The CLA-methyl ester standard (250 mg; Sigma Chemical Co., St. Louis, MO) was dissolved in 25 mL of HPLC-grade hexane. Concentrations of 1.0, 2.0, 4.0, 5.0, 10.0, 20.0, 40.0, 50.0, 60.0, 80.0, and 100.0 µg/mL (ppm) were prepared and measured by HPLC using the same conditions as described for the lipid extracts. The calibration curve was built and used in quantifications of CLA in the lipid extracts. Recovery of CLA was checked by spiking a starch sample (~10 g) with 3 mL of the CLA standard (10 mg/mL), and performing a re-extraction by the Bligh and Dyer (1959) method. The extracted CLA that was dried under nitrogen gas, dissolved in methanol, and measured by HPLC under the same conditions was used for the standard and the lipid extracts analysis. The linearity was checked and found to be up to 60 ppm; the detection limit was 1 ppm. The calibration curve is shown in Figure 2Go and the linearity is represented by R2.



View larger version (15K):
[in this window]
[in a new window]
  		Figure 2. Conjugated linoleic acid calibration curve as measured at 245 nm by HPLC.

 
Recoveries of CLA were determined by adding 10 µL of 100-ppm standard CLA to a starch sample, followed by 3 replicate complete analyses. Each sample was injected in triplicate into the HPLC on 3 separate days. Recoveries and confidence limits of CLA are shown in Table 4Go. The mean recovery values were 98.4, 98.2, and 94.0% for d 1, 2, and 3, respectively.


View this table:
[in this window]
[in a new window]
  		Table 4. Recoveries of conjugated linoleic acid (CLA; addition of 1000 µg) on d 1, 2, and 3.
 
Statistical Analyses
Data were analyzed using the ANOVA procedure of SAS (version 7, 1998; SAS Institute, Inc., Cary, NC). Duncan’s multiple range test was applied to determine significance between different treatments.


   RESULTS AND DISCUSSION
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
CONCLUSIONS
 REFERENCES
 
Milk and Milk Products
Influence of heating of milk on CLA and trans isomer contents.
Trans isomer and CLA contents of milk and milk products are shown in Table 5Go. Infrared spectra of the lipid methyl ester of the fat samples are illustrated in Figure 3Go. The results indicate that milk pasteurization (85 ± 1.0°C for 16 s or 95 ±1.0°C for 5 min) and UHT (140 ±1.0°C for 4 s) had no significant (P > 0.05) influence on trans isomer formation. On the other hand, pasteurization at 63 ± 1.0°C for 30 min or heating in a microwave at 95.8 ±1.0°C for 5 min caused a significant (P < 0.05) increase in the trans isomers. For example, the trans isomer values for raw, pasteurized (95.8 ± 1.0°C), and microwaved milk were 1.69, 1.76, and 2.22% (as methyl elaidate), respectively.


View this table:
[in this window]
[in a new window]
  		Table 5. Effect of different heat treatments and commercial shelf life storage of milk and milk products on conjugated linoleic acid (CLA) and trans isomers (as % elaidic acid or methyl elaidate) contents.1
 


View larger version (20K):
[in this window]
[in a new window]
  		Figure 3. Fourier-transform infrared spectra of methyl elaidate standard of 0.2 mg/mL, microwave-heated milk, and UHT milk.

 
The significant increase in trans isomer formation due to microwave heating could be attributed to the unique mechanism of microwave heating. Moreover, heating for a prolonged period (30 min) at relatively low temperature (63 ± 1.0°C) under aerobic conditions seems to contribute to lipid oxidation more than heating at higher temperature for 5 min. This unexpected result may be explained as follows: heating at 63°C is not effective in expelling the dissolved oxygen in the liquid milk, whereas heating above 80°C caused a rapid escape of the dissolved oxygen.

It is worth noting that low-temperature, long-time pasteurization was not more effective in trans isomer formation than UHT (which uses a very short time) but was more effective than heating at 95 ± 1.0°C for 5 min, because it is known that isomerization of oils generally occurs as a result of severe heating (Semma, 2002). It is assumed that this isomerization was due to the action of microbes or specific enzymes found in raw milk, especially because heating treatment in this experiment was very slow (heating in glass beakers at 63 ± 1.0°C for 30 min in a water bath of 70 to 80°C). The increase of trans isomers of about 30% after microwaving for 5 min demonstrates the detrimental effect of this energic electronic heating.

Conjugated linoleic acid contents of raw and heated milk (Table 5Go) indicated that there was no significant (P > 0.05) difference among the different heating treatments with the exception of microwave heating and UHT, which caused significant decrease of the CLA content. The average values of CLA for raw and microwave-heated milk were 5.67 and 4.48 mg/g of fat, respectively.

On the other hand, refrigerated storage at 5.0 ± 1.0°C had no significant effect on CLA content of milk, except for pasteurized (at 85 ± 1.0 for 16 s) milk stored for 3 d, which resulted in a decrease in CLA content. The CLA levels generally were in agreement with the results reported by others (Warner et al., 1992; Lin et al., 1998; DeMan, 1999; Ma et al., 1999).

The tendency toward a decrease in CLA content upon heating could be attributed to fat oxidation resulting in formation of hydroperoxides, which might cause CLA conversion or degradation.

The significant decrease (P < 0.05) in CLA content of microwave-heated milk samples compared with raw milk could be due to the scavenging act of CLA toward free radicals that are formed as a result of lipid oxidation (Okada et al., 1996; Leung and Liu, 2000).

Yogurt and Labaneh
The CLA and trans isomers contents of yogurt and labaneh are presented in Table 5Go. The results of the trans isomers levels calculated as percentage of methyl elaidate indicated that processing steps or refrigerated storage of yogurt and labaneh had no significant effects (P > 0.05) on trans isomer formation. This result is in agreement with the results of Jenkins et al. (2003) and Jenkins (1992), who reported that storage conditions of fermented dairy products did not affect the level of CLA.

The results in Table 5Go show that conversion of milk to yogurt and yogurt to labaneh had no significant effect on either trans fatty acid or CLA content. Labaneh produced by conventional method (strained in cloth) showed a relative increase in its CLA level compared with raw milk, yogurt, and labaneh strained by separator. This increase could be attributed to the straining time of labaneh in cloth (which takes more than 18 h at room temperature) that may enhance the fermentation process, and hence, formation of CLA through microbial biohydrogenation. Because biohydrogenation is an enzymatic process in which microbial isomerase enzymes (that may be produced during fermentation) are needed to convert cis-9 and cis-12 of the fatty acid (C18:2) to CLA, the cis-9 and trans-11 form (Jenkins, 1992).

On the other hand, the significant decrease in CLA content of yogurt stored with refrigeration for 7 d compared with that of raw milk may be explained by the scavenging effect of CLA against free radicals that are formed because of lipid oxidation (Okada et al., 1996; Leung and Liu, 2000). However, the CLA content of labaneh was not affected by refrigerated storage conditions as shown in Table 5Go. This result could be explained by the assumption that the rate of CLA formation due to prolonged fermentation was in balance with its conversion and degradation during storage periods.

Brined White Cheese (Nabulsi)
The effects of processing and heating of brined white boiled cheese (Nabulsi) on the CLA and trans isomer contents are presented in Table 6Go. Four chromatograms are presented in Figure 4Go: 2 chromatograms showing the levels of CLA in the fat extracted from microwave-heated and raw sheep milk, 1 chromatogram for the standard CLA methyl ester, and 1 chromatogram for the fatty acid methyl esters. The chromatograms illustrate the successful separation of CLA from fatty acid methyl esters.


View this table:
[in this window]
[in a new window]
  		Table 6. Effect of different heat treatments and commercial shelf life storage of white brined cheese (Nabulsi) on its conjugated linoleic acid (CLA) and trans isomers (as % methyl elaidate) contents.1
 


View larger version (17K):
[in this window]
[in a new window]
  		Figure 4. High performance liquid chromatograms of conjugated linoleic acid (CLA) from (A) microwave heated brined white cheese (Nabulsi), (B) raw sheep milk, (C) CLA standard 0.4 ppm, and (D) standard fatty acid methyl ester. The conditions of the HPLC were as described in Materials and Methods.

 
The results in Table 6Go show that no significant difference exists in trans isomer content of fat extracted from raw sheep milk and that extracted from cheese samples heated by a conventional method (boiling on gas stove). On the contrary, reheating of cheese in a microwave oven increased the trans isomer content significantly. For example, the values of trans isomers for raw sheep milk, boiled cheese, and microwave-heated cheese were 1.66, 1.79, and 2.18% (calculated as methyl elaidate), respectively. Furthermore, reheating time of cheese had a significant effect on increasing the trans isomers level. The values for cheese reheated in a microwave for 5 and 10 min were 2.18 and 3.17%, respectively.

It is worth noting that the increase in trans isomers of microwave-heated cheese compared with that of raw milk or cheese boiled on gas stove could be because isomerization needs high activation energy that is achieved at high temperature (Taylor et al., 1983; Semma, 2002). Microwave ovens have the power to provide the activation energy required to form the trans isomers. The results in Table 6Go show that the processing steps of the Nabulsi cheese (renneting, pressing, and boiling) did not cause any significant changes in trans isomers level.

The results show that microwave reheating had a significant effect on lowering the CLA content of the reheated cheese. Furthermore, reheating time had a significant effect on CLA levels. For example, the values of CLA in the cheese microwaved for 5 and 10 min were 4.96 and 2.93 mg/g of fat, respectively. The decrease in CLA content of cheese reheated in a microwave oven compared with that of the raw milk, fresh curd, and boiled cheese may be partially attributed to acceleration of lipid oxidation by microwaves or production of pro-oxidants (Ha et al., 1989; Lin et al., 1998).

The results of reheating Nabulsi cheese stored in cans for 1 mo at room temperature (18 ± 1° C) indicated that storage has no significant effect on trans fatty acid isomers or CLA content compared with the results before storage. The insignificant effect of storage on CLA contents is in agreement with the result found by Lin et al. (1998), which demonstrated that processing steps and storage had a minor influence on the formation of CLA in Cheddar cheese.


   CONCLUSIONS
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 CONCLUSIONS
REFERENCES
 
With the exception of microwave heating, heat treatments and refrigerated storage of milk and dairy products did not cause significant changes in the trans fatty acid isomer content. Microwave heating caused an increase in trans fatty acid from 1.69% in raw milk to 2.22% in microwave-heated milk (an increase of 31%) and a ~19% increase in milk pasteurized at 63 ± 1.0° C for 30 min. This increase could have an adverse effect on health. Likewise, with the exception of microwaving, none of the heat or storage treatments used caused significant changes in CLA content. Microwaving caused a significant decrease in CLA content in all products compared with freshly boiled cheese. The percentage decrease of CLA in cheese microwaved when immersed in water for 5 min was 21%, whereas CLA content was decreased by only 1% after boiling of cheese in water on a stovetop. Microwaving of cheese directly for 10 min decreased the CLA content by 53%. This means that the microwave-heated cheese has lost one of the most valuable anticarcinogenic components.

Received for publication August 28, 2004. Accepted for publication December 20, 2004.


   REFERENCES
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS AND DISCUSSION
 CONCLUSIONS
 REFERENCES
 


Addis, P. B. 1990. Coronary heart disease: An update with emphasis on dietary lipid oxidation products. Nutr. Rev. 62:7–10

Addis, P. B., and G. J. Warner. 1991. The potential health aspects of lipid oxidation products in food. Pages 77–119 in Free Radicals and Food Additives. O. I. Aruoma and B. Halliwell, ed. Taylor & Francis Ltd., London, UK.

Albi, T., A. Lanzon, A. Guinda, M. Leon, and M. C. Perez-Camino. 1997a. Microwave and conventional heating effects on thermoxidative degradation of edible fats. J. Agric. Food Chem. 45:3795–3798.

Albi, T., A. Lanzon, A. Guinda, M. C. Perez-Camino, and M. Leon. 1997b. Microwave and conventional heating effects on some physical and chemical parameters of edible fats, J. Agric. Food Chem. 45:3000–3003.

Ames, B. N., L. S. Gold, and W. C. Willett. 1995. The causes and prevention of cancer. Proc. Natl. Acad. Sci. 92:5258–5265.[Abstract/Free Full Text]

Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911–917.

Boué, C., N. Combe, C. Billeaud, and B. Entressangles. 2000. Nutritional implication of trans fatty acids during perinatal period in French pregnant women. Human Nutrition and Animal Feeding, Joint International Congress and Expo, Lipids, Fats and Oils Opportunities and Responsibilities in the New Century, Wurzburg, Germany.

Chin, S. F., W. Liu, J. M. Storson, Y. L. Ha, and M. W. Pariza. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Comp. Anal. 5:185–197.

Christie, W. W. 1992. The analysis of fatty acids. Pages 65–83 in Gas Chromatography and Lipids: A Practical Guide. Oily Press Ltd., Dundee, UK.

Conacher, H. B. S. 1976. Chromatographic determination of cis-trans monoethylenic unsaturation in fats and oils–A review. J. Chromatogr. Sci. 14:405–411.[Medline]

Decareau, R. V. 1992. Microwave science and technology. Pages 1772–1778 in Encyclopedia of Food Science and Technology. Vol. 3. Y. H. Hui, ed. John Wiley and Sons, Inc., Mississauga, Ontario, Canada.

DeMan, J. M. 1999. Pages 33–110 in Principles of Food Chemistry. 3rd ed. Aspen Publishers, Inc., Frederick, MD.

Guillen, M. D., and N. Cabo. 1997. Infrared spectroscopy in the study of edible oils and fats. J. Sci. Food Agric. 75:1–11.

Gunstone, F. D. 1986. Chemical properties. Pages 449–484 in The Lipid Handbook. F. D. Gunstone, J. L. Harwood, and F. B. Padley, ed. Chapman and Hall, London, UK.

Ha, Y. L., N. K. Grimm, and M. W. Pariza. 1989. Newly recognized anticarcinogenic fatty acids: Identification and quantification in natural and processed cheeses. J. Agric. Food Chem. 37:75–81.

Humeid, M., and S. Tukan, S. 1986. Towards the development of the traditional method of white boiled "Nabulsi" cheese. J. Dirasat 13:19–29.

Humeid, M., S. K. Tukan, and M. I. Yamani. 1990. In-bag steaming of white brine cheese as a method of preservation. Milchwissenschaft 45:513–516.

Jenkins, T. C. 1992. Symposium: Advances in ruminant lipid metabolism: Lipid metabolism in the rumen. J. Dairy Sci. 76:3851–3863.

Jenkins, T. C., V. Feliner, R. K. McGuffey. 2003. Monensin by fat interaction on trans fatty acid in cultures of mixed ruminal micro-organisms grown in continuous fermentors fed corn or barley. J. Dairy Sci. 86:324–330.[Abstract/Free Full Text]

Jensen, R. G., A. M. Ferris, and C. J. Lammi-Keefe. 1991. The composition of milk fat. J. Dairy Sci. 74:3228–3243.[Abstract]

Jensen, R. G., A. M. Ferris, C. J. Lammi-Keefe, and R. A. Henderson. 1990. Lipids of bovine and human milks: A comparison. J. Dairy Sci. 73:223–230.[Abstract]

Kumar, N., and O. P. Singhal. 1991. Cholesterol oxides and atherosclerosis: A review. J. Sci. Food Agric. 55:497–510.

Leung, Y. H., and R. H. Liu. 2000. Trans-10, cis-12-conjugated linoleic acid isomer exhibits stronger oxyradical scavenging capacity than cis-9, trans-11-conjugated linoleic acid isomer. J. Agric. Food Chem. 48:5469–5475.[Medline]

Lin, H., D. Boylston, L. O. Luedecke, and T. D. Shultz. 1995. A survey of the conjugated linoleic acid contents of dairy products. J. Dairy Sci. 78:2358–2365.[Abstract]

Lin, H., T. D. Boylston, L. O. Luedecke, and T. D. Shultz. 1998. Factors affecting the conjugated linoleic acid content of Cheddar cheese. J. Agric. Food Chem. 46:801–807.

Ma, D. W. L., A. A. Weirzbicki, C. J. Field, and M. T. Clandinin. 1999. Conjugated linoleic acid in Canadian dairy and beef products. J. Agric. Food Chem. 47:1956–1960.[Medline]

Mossoba, M. M., R. E. McDonald, J.-Y. T. Chen, D. Y. Armstrong, and S. W. Page. 1990. Identification and quantification of trans-12-octadecadienoic acid methyl ester and related compounds in hydrogenated soybean oil and margarines by capillary GC/matrix isolation/Fourier transform IR. J. Agric. Food Chem. 38:86–92.

Mossoba, M. M., R. E. McDonald, and E. R. Prosser. 1993. GC matrix-isolation Fourier-transform infrared spectroscopic determination of trans-monounsaturated fatty acid methyl esters in partially hydrogenated menhaden oil. J. Agric. Food Chem. 41:1998–2002.

Okada, Y., M. Kaneko, and H. Okajima. 1996. Hydroxyl radical scavenging activity of naturally occurring furan fatty acids. Biol. Pharm. Bull. 19:1607–1610.[Medline]

Parodi, P. W. 1999. Conjugated linoleic acid and other anticarcinogenic agents of bovine milk fat. J. Dairy Sci. 82:1339–1349.[Abstract]

Potter, N. N., and J. H. Hotchkiss. 1996. Irradiation, microwave, and ohmic processing of foods. Pages 245–379 in Food Science. 5th ed. Chapman and Hall, New York, NY.

Semma, M. 2002. Trans fatty acids: Properties, benefits and risks. J. Health Sci. 48:7–13.

Svensson, L., L. Sisfontes, G. Nyborg, and R. Blomstrand. 1982. High performance liquid chromatography and glass capillary gas chromatography of geometric and positional isomers of long chain monounsaturated fatty acids. Lipids 17:50–59.

Taylor, S. C., C. M. Berg, N. H. Shoptaugh, and E. Traisman. 1983. Mutagene formation in deep-fat fried food as a function of frying conditions. J. AOCS 60:576–580.

Toschi, T. G., P. Capella, C. Holt, and W. W. Christie. 1993. A comparison of silver ion HPLC plus GC with Fourier-transform IR spectroscopy for the determination of trans double bonds in unsaturated fatty acids. J. Sci. Food Agric. 22:261–265.

Ulberth, F., and H.-J. Haider. 1992. Determination of low level trans unsaturation in fats by Fourier transform infrared spectroscopy. J. Food Sci. 57:1444–1447.

Walker, R. O. (ed.) 1978. Official and Tentative Methods of the American Oil Chemists Society: Isolated trans isomers, infrared spectrophotometric method. Am. Oil Chem. Soc. CD 14–61.

Warner, S. A., L. O. Luedecke, and T. D. Shultz. 1992. Determination of conjugated linoleic acid content and isomer distribution in three Cheddar-type cheeses: Effect of cheese cultures, processing, and aging. J Agric. Food Chem. 40:1817–1821.

Willet, W. C., and A. Ascherio. 1994. Trans-fatty acids: Are the effects only marginal? Am. J. Pub. Health 84:722–724.

Yamaoka, R., M. Tokoro, and K. Hayashita.1987. Determination of geometric configuration in minute amounts of highly unsaturated termite trail pheromone by capillary gas chromatography in combination with mass spectrometry and Fourier-transform infrared spectroscopy. J. Chromatogr. 399:259–267.


This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
L. M. Rodriguez-Alcala and J. Fontecha
Hot Topic: Fatty Acid and Conjugated Linoleic Acid (CLA) Isomer Composition of Commercial CLA-Fortified Dairy Products: Evaluation After Processing and Storage
J Dairy Sci, May 1, 2007; 90(5): 2083 - 2090.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Interpretive Summary
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herzallah, S. M.
Right arrow Articles by Al-Ismail, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herzallah, S. M.
Right arrow Articles by Al-Ismail, K. M.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS