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* University of Wisconsin, Madison 53706, Department of Dairy Science
Biological Systems Engineering Department
Corresponding author:
Roger W. Palmer; e-mail:
rwpalmer{at}facstaff.wisc.edu.
| ABSTRACT |
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Key Words: stall use cow preference stocking density freestall base
Abbreviation key: 100%SD = higher stocking density, END = stalls on row ends or next to a different stall base, NOTEND = stalls not on row ends or next to different stall bases, LowSD = low stocking density, MATR1 = mattress type 1, MATR2 = mattress type 2, RH% = relative humidity, RLOC = barn side, STLLOC = stall location within stall base section, WDIST = distance to water, XPOSR = length of time cows exposed to stall bases
| INTRODUCTION |
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Monitoring stall use percentages provides an indication of cow preference and comfort level. Videotape images and time-lapse photography have been used to observe stall use and cow activity, lying or standing in stalls, or feeding (Albright and Timmons, 1984; Krohn and Munksgaard, 1993; Herlin, 1997; Haley et al., 1999; Overton et al., 2000). Observing cow activity through videotape images versus on-site removes the potential influence of human presence on cow activity.
Stall base and design influence cow acceptance and comfort. Stalls providing a comfortable, conforming base, and designed to allow for ease of rising and lying movements are used more by the cows (Bickert and Ashley, 1991). Incorrect stall design inhibited stall use (Feddes et al., 1995; Muller and Botha, 1997). Thickness or depth of the stall base, 3 to 6 in (7.6 to 15.2 cm) for mattresses, improved durability and cow comfort (Bickert and Ashely, 1991; House, 1998).
Stall usage research has been reported for cows exposed to sand, mattress, and rubber-mat based stalls. Sand was considered the best in providing comfort (Tucker and Weary, 2001). Because sand settles and separates from manure, it requires a specially designed manure management system (Bickert and Ashley, 1991; Leonard and OConnell, 1997; Thoreson et al., 2000). Rodenburg and House (2000) review of previous research found lying percentages were higher on rubber-filled mattress stalls (43.3%) versus sand stalls (26.4%).
House et al. (1994) and Chaplin et al. (2000) found rubber-filled mattresses had a higher frequency of use than rubber-mat bases. However, conflicting results were reported by Rodenburg et al. (1994), who found average lying percentage to be higher on rubber mats versus rubber-filled mattresses.
Length of time cows are exposed to particular stall bases influences lying and occupancy percentages. Cows exposed to rubber mats had a longer adjustment period than those exposed to mattresses (Chaplin et al., 2000). Occupancy of waterbed-based stalls started low, but increased with time as cows became accustomed to them (Sonck and Daelemans, 1999; Rodenburg and House, 2000). Occupancy rates on waterbeds were lower initially, but were the same as rubber-filled mattresses at the end of the trial (Sonck and Daelemans, 1999).
Temperature may play a role in cow preference for a particular stall. Earth with deep bedding, and rubber-mat-based stalls were chosen over carpet and concrete-based stalls during summer and winter trials (Gebremedhin et al., 1981). Thoreson et al. (2000) found sand (60.8% occupancy) was favored over mattresses (19.4 to 32.5% occupancy) and rubber mats (12.3% occupancy) during a summer trial. Sand stalls were found to be used (occupied) less during winter (27%) compared to summer (60.8%) (Thoreson et al., 2000).
Natzke et al. (1982) found stall use was less for stalls on the interior row of the barn and for stalls located on the ends of the exterior and interior rows of the barn. However, Sonck and Daelemans (1999) found stall base position and location in the barn did not affect occupancy level. Conflicting reports show no consistent effect of stall location on stall use.
Increased stocking density (number of cows per stall multiplied by 100) above 100% may not result in a loss of cow comfort or stall use. Maximum stall use for a 100% stocked freestall barn was 86%, suggesting stocking density of 110 to 115% should not affect stall availability (Gebremedhin et al., 1981). House et al. (1994) reported 95% lying in a freestall barn with mattress bases and a 58 to 69% stocking density.
Previous studies have shown stall base, design, location, length of exposure time, temperature, and stocking density influence stall use. Different stall bases have been evaluated with conflicting stall usage results. The objective of this study was to compare stall occupancy and cow position (lying in or occupying stall) for six factors affecting stall use: stall base, distance to water (WDIST), stall location within stall base section (STLLOC), barn side (RLOC), inside barn temperature, and length of time cows exposed to stall bases (XPOSR).
| MATERIALS AND METHODS |
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Figure 1
shows the freestall barn layout and observation recording sheet (not to scale). The stalls were identified by stall base type and stall number. Video cameras panned the stalls in a specific order and the stall numbers were assigned accordingly for identification purposes. The RLOC was defined as the following: stalls located near the feed alley were considered on the interior row, while stalls located on the outside of the building were on the exterior row. The two sides of the barn were: 1) the north side, which had a low stocking density (LowSD) and houses cows milked by a robot milker; and 2) the south side, had a higher stocking density (100%SD) and houses cows milked 2X in a conventional parlor. Cows on the LowSD side can only enter the feed alley area by passing through the robot and leave the feed alley through a one-way gate located on the west end of the barn. Both sides had access to, and spent time at, the feed bunk after being milked. Because 100%SD cows were batch milked, and LowSD cows were sequentially milked, the percentage of cows at the feed bunk at one time was higher for 100% SD. The STLLOC was defined as the following: stalls on row ends or next to a different stall base were classified as END, and the remainder was classified as NOTEND.
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The stall bases included rubber mat (Comfort Zone-Milk Mat, Alfa Laval Agri, Kansas City, MO), waterbed (Atlanta Cow Waterbeds, Scottdale, GA), mattress type 1 (MATR1 = Pasture Mat Mattresses, Seaforth, Ontario, Canada), mattress type 2 (MATR2 = Comfy Cow Free Stall Mattresses, Byron Center, MI), concrete, and sand. Rubber mats were solid, ethylene vinyl acetate, material. The waterbed based stalls were made with two layers of a vulcanized rubber (100% natural). Each layer had a fiber layer and six plies of rubber, and each stall was individually filled with three gal (11.4 l) of calcium chloride and 13 gal (49.2 l) of water. The MATR1-based stalls had three layers: the top layer was needle-punched polypropylene impregnated with wax to increase water shedding; the second layer was either a 1 in (2.5 cm) foam pad or a 0.75 in (1.9 cm) felt pad; and the bottom layer was a multi-celled, rubber crumb (crushed tire) filled mattress. The MATR2-based stalls contained a polyethylene interior of recycled foam and vinyl from the automobile industry with a Tafcoat waterproof cover.
Washed mason sand was used in sand stalls. Stalls were cleaned and leveled at milking time, and sand was added once a week to establish a level surface, from the top of the stall curb to the bottom of brisket board, for an approximate depth of 8 to 9 in (20.3 to 22.9 cm). Sand usage was not recorded. Each stall base was grouped 3 to 7 stalls per section, and sections randomly placed in each row on each side. The sand stalls were not used on the LowSD because of concerns of sand settling in the top portion of the gravity-flow manure-handling system. The MATR1, MATR2, waterbed, and concrete-based stalls were bedded with sawdust twice per week and soiled bedding was removed following the scheduled milking (parlor system) or human-intervention time (automatic milking system). Stall bedding dates were not recorded, so stall usage by days since bedding was not analyzed. Rubber mat bases were refastened to the concrete base in July and bedding of these stalls was discontinued at the manufacturers request.
An ADT Security Systems, Inc. (Menlo Park, CA) closed-circuit monitor camera system, with one camera on each side, was used to observe activity 24 h per d. Digital images were recorded using a Pelco VCR and Simplex Monochrome Multiplexor.
Four observation times per day, 2:00 p.m., 8:00 p.m., 4:00 a.m., and 9:00 a.m., were observed from May 9, 2001 to February 9, 2002. The 9-mo data collection period was selected to represent normal seasonal temperature and relative humidity (RH%) variation in the Midwest region of the United States. The 2:00 p.m. observation time was chosen to allow for potential delays in the regularly scheduled 12:00 p.m. tape change. Lights were off from 9:30 p.m. to 3:30 a.m. The 4:00 a.m. observation time was chosen to observe stall use after the lights were turned on in the freestall barn. The 8:00 p.m. and 9:00 a.m. observation times were chosen to be 2 hours after the scheduled parlor milking time, to allow for variation in milking time, and allow cows time to eat after they returned from the parlor.
The five, six, and eight-hour intervals were selected to make the assumption that the observations were independent from each other. This was determined from a preliminary analysis of data collected every 15 min that showed no stall was being occupied consistently for a 5 to 6-h period. Therefore, all unoccupied stalls were assumed equally appealing (Hemken, 2002, personal communication). Similarities between means and standard deviations of each observation time, for each status, supported the conclusion that the four observation times were similar and accurately characterized stall occupancy across a day.
The following steps were used to view videotape images and record observations: First, stalls were observed in sequential order on 100% SD and assigned one of the following statuses: lying in stall, standing half-in-and-out of stall, standing in stall, empty stall, or unsure status. Unsure was assigned to stalls that could not be accurately categorized as any of the first four. Stalls were then observed similarly on the LowSD side.
Five HOBO H8 Pro Series (Onset Computer Corporation, Pocasset, MA) sensors recorded temperature every 15 minutes. One sensor was located in each quadrant of the barn, northwest (NW), northeast (NE), southwest (SW), southeast (SE) 38 to 40 ft (11.6 to 12.2 m) from each end wall, and 9 to 10 ft (2.7 to 3.0 m) above the alley floor surface over the front of the interior row of stalls. One sensor was located in a radiation shield outside (OS) the barn on the north side, and 21 ft (6.4 m) from the barn and 3.5 ft (1.1 m) above the ground surface. Figure 1
shows the approximate location of the four inside sensors (NW, NE, SW, and SE).
Each stall was assigned temperature data from the closest sensor for each observation. Due to the different systems for collecting stall occupancy and barn temperature data, the actual stalls status observation time varied from the temperature by 0 to 10 min. Stalls 1 to 13 and 40 to 50 on 100%SD were assigned the temperature recorded from sensor SE. Stalls 14 to 39 on 100%SD were assigned the temperature recorded from the sensor SW. Stalls 1 to 16 and 43 to 54 on LowSD were assigned the temperature recorded from the sensor NW. Finally, stalls 17 to 42 on LowSD were assigned the temperature recorded from the sensor NE.
A total of 961 stall use observations from 100%SD and 983 stall use observations for LowSD were used in the analysis for the 9-mo period. A total of 119 complete observations (both sides) and 34 half observations (one side) were missing. The reasons for missing observations were: image not recorded, tape not switched on time, camera(s) not panning/moving across stalls, camera(s) not running/recording, and not enough light to see stall occupancy.
Percentages of each status were calculated as the number of stall-day-status observations divided by total number of stall-day observations for the different categories or factors tested across the 9-mo period. Stall occupied percentages were calculated using the number of stall-day-lying, standing, and standing half-in-and-out observations divided by total number of stall-day observations.
Percentages for the different factors were analyzed using logistic regression with the GENMOD Procedure in Statistical Analysis Software (SAS, SAS Institute, Cary, NC, 1999) and contrast statements were used to determine significant differences between percentages (Stokes et al., 1995). Each side was analyzed separately to eliminate errors due to different stocking densities of the 100%SD and LowSD sides. Stocking densities were a function of herd management and not intentionally set as part of the studys design.
The CATMOD Procedure in SAS was used to model lying and occupied as binary outcomes. Independent variables considered continuous were XPOSR, WDIST, and temperature. Independent variables considered categorical were stall base, RLOC, STLLOC, and side. Any 2-way interactions considered biologically significant or meaningful were also analyzed.
| RESULTS AND DISCUSSION |
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The stall base effect had significant (P < 0.0001) interactions with RLOC, STLLOC, side, XPOSR, WDIST, and temperature. This indicates a cows preference to lie down on a particular stall base varied for each of these factors. The RLOC effect had significant (P < 0.0001) interactions with temperature and side indicating lying percentages on interior and exterior rows were different for the temperature intervals and two sides. This may reflect cows tendency to avoid outside rows of stalls during periods of high temperatures. The WDIST effect had a significant (P < 0.0001) interaction with side. The cows preference to lie in a stall a certain WDIST away varied by side. RLOC, side, and XPOSR were not significant predictors (P < 0.05), but were kept in the model due to interaction with other factors.
The WDIST effect best explained the variation in stall occupied (P < 0.0001), but stall base, RLOC, STLLOC, XPOSR, and temperature effects were also significant (P < 0.01) predictors. The stall base effect had a significant (P < 0.001) interaction with RLOC, STLLOC, side, XPOSR, WDIST, and temperature. The RLOC effect had a significant (P < 0.0001) interaction with temperature and side. The WDIST and STLLOC effects had significant (P < 0.01) interactions with side.
Table 2
shows the stall occupancy percentages by stall base for each side. The current study showed cows on the 100%SD side spent the highest (P < 0.05) percentage of time lying in sand (68.7%). Mattresses (MATR1 [65.2%] and MATR2 [57.4%]) followed closely as the next most preferred (P < 0.05), followed by waterbed (45.4%), rubber mat (32.9%), and the least preferred (P < 0.05) was concrete (22.8%). Mattresses (MATR1 and MATR2) had the highest (P < 0.05) stall occupied percentages (88.3% and 84.1%) followed by sand (79.0%), rubber mat (64.8%), waterbed (61.6%), and concrete (38.7%). The mattresses higher stall occupied percentage over all other stall bases, including sand, was caused by the higher percentage of time cows spent standing on these stall bases. The 100%SD results show cows spend more time lying in sand stalls, but occupied mattress based stalls more, which implies these two measures can be expected to rank cow preference for the different bases differently. Both measures consistently ranked concrete to be inferior to all other surfaces; mattresses superior to waterbed and rubber mat; and MATR1 superior to MATR2 on both sides. Side had a small effect on cow preference rankings, for the different stall bases, as shown by only rubber mat and waterbed having rank variation. This finding may be helpful in comparing cow preference for different stall bases across different experiments.
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Lying and occupied percentages for sand stalls were higher than in previous studies. Visser (1994) showed a 10% occupancy rate with sand alone when compared to other stall bases of sand with a canvas cover (60%) and maize choppings with a canvas cover (21%). However, a 38.4% occupancy rate for sand was found by Thoreson et al. (2000). The 40% to 69% higher lying and occupied percentages found in the current study may be attributed to maintenance of sand levels in stalls, sand quality, alternative stall options, and or time of day of the observation. Previous studies measures of occupancy (lying, standing, and/or standing half in and out) were not defined. This may have contributed to differences found between current and previous results.
Mattresses (MATR1 and MATR2) stall occupied percentages were similar to those found in previous studies. Rodenburg and House (2000) reviewed results of 86.2% occupancy and Sonck and Daelemans (1999) found greater than 50% occupancy on rubber-filled mattresses.
Lying percentage trends across stall bases differed from previous studies. Rubber-filled mattresses resulted in 43.3% lying versus 26.4% in sand in a study reviewed by Rodenburg and House (2000). The depth of sand in the sand based stalls and sand quality may have attributed to the differences in lying percentages between current and previous studies. The waterbed stalls percentage (45.4%) was similar to the 45.95% average lying percentage reported by Sonck and Daelemans (1999). The rubber mat stalls percentage (32.9%) was slightly lower than the 51.08% reported by Sonck and Daelemans (1999).
Order of stall occupied percentages for mattresses (MATR1 and MATR2), rubber mat, sand, and waterbed was similar to previous studies. Chaplin et al. (2000), House et al. (1994), and Rodenburg and House (2000) found occupancy percentages to be higher on rubber-filled mattresses versus sand. Rodenburg and House (2000) and Sonck and Daelemans (1999) found lower occupancy rates on waterbeds compared to rubber-filled mattresses. Muller and Botha (1997) found cows would rise more and lie down less if the stall base was hard.
Cook (2002) proposed the use of a cow comfort index (CCI = number of cows standing divided by the total number of cows standing and lying in a stall) to evaluate cow comfort for different stall bases. Bases with lower CCI values were considered more comfortable than stall bases with a higher CCI value. The CCI values were calculated using the current study results (Table 2
), and ranged from 13% (sand) to 49% (rubber mat) on 100%SD and from 24 (MATR1) to 38% (rubber mat) on LowSD. Current and previous research used stall usage as an indicator of cow preference. The CCI index appears to unfairly penalize bases with a high percentage of cows standing in the stalls. The CCI index values calculated with the current data consistently ranked stall bases differently for cow comfort from stall bases ranked on stall use in the current and previous studies. The sand CCI value (13%) implied it was twice as good as MATR1 (26%) on 100%SD. The waterbed based stalls (32%) were ranked over MATR2 (26%), and concrete (41%) over rubber mat (49%) on 100%SD. Waterbed (24%) was ranked equal to MATR2 (24%) and concrete (36%) over rubber mat (38%) on the LowSD. Based on the current studys ranking of cow preference by stall usage, an alternative method of measuring cow preference and comfort is needed. This alternative method to evaluate stalls for cow comfort should incorporate percentage of cows lying in and occupying stalls and stocking density.
Figure 2
shows the percentage of stalls with cows standing, cows lying, or stalls occupied of interior and exterior rows by side. The current study results show cows preferred to lie down, stand in, and occupy stalls on the exterior row on both sides of the barn (P < 0.05). The exterior row on 100%SD had 52.8% lying, 14.8% standing, and 73.4% occupied. The exterior row on LowSD had 30.1% lying, 8.7% standing, and 41.9% occupied. Cows may have occupied stalls located near the curtain sidewall for improved ventilation or temperature regulation.
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Location results were in agreement with Natzke et al. (1982), which found stall use was less in stalls on the interior row and located on the ends of the row. The stall base x RLOC and stall base x STLLOC interactions were significant, indicating cow preference for a stall base varied by location. The RLOC x temperature interaction was also significant, therefore stall use for stalls on the interior and exterior rows varied by temperature.
Table 3
shows the percentage of stalls with cows lying for each stall base by month for each side. The length of XPOSR influenced lying percentages. Lying percentages varied month to month for stall bases. The stall base x XPOSR interaction was significant (P < 0.05), which indicates stall use for particular stall bases was different for different levels of XPOSR. Sand had the largest variation by month (43% June to 75.8% December). Temperature and level of sand in the stalls may have attributed to this variation. On 100%SD, gradual declines in cow preference were observed for rubber mat. The rubber mat based stalls started at 49.3% lying in May and ended at 28.6% in February. Use of waterbed based stalls increased with time, the percentage lying increased from 43.7% in May to 54.0% in February. Percentage lying for waterbed was similar to MATR1 and MATR2 by February. Mattresses (MATR1 and MATR2) lying percentages had the smallest fluctuation over the 9-mo period.
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Table 4
shows the percentage of stalls occupied for each stall base by month for each side. The length of XPOSR influenced stall occupied percentages. Trends were similar to lying percentages. The concrete based stalls consistently had the lowest occupied percentages from month to month. The MATR1 based stalls were consistently the highest from month to month and MATR2 and sand ranked high throughout the 9-mo period with MATR1 finishing at 91.0%, MATR2 at 78.3%, and sand at 82.0%. There was a gradual increase in occupied percentages for waterbed and concrete. The waterbed based stalls started at 56.1%, ended at 66.7% and concrete started at 24.6% and ended at 43.5%. The rubber mat based stalls experienced a gradual decrease starting at 76.2% occupied and falling to 59.8% in February. Trends in stall occupied rates for the LowSD, from month to month, were similar to LowSD lying trends.
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Figure 4
shows the percentage of stalls with cows lying on the 100%SD side for stall base by temperature (°F) interval. The temperature effect influenced lying and stall occupied percentages for each stall base; stall use decreased as temperature increased. This was supported by the significant (P < 0.05) stall base x temperature, which indicates a cow preference for different stall base types at different temperature intervals. Cows preferred (P < 0.05) to lie down on sand (79.2 to 72.3%) when temperature was 21 to 60°F (-6.1 to 15.6°C) and MATR1 (80.9 to 64.3%) when temperature was 1 to 20°F (-17.2 to -6.7°C) and 61 to 100°F (16.1 to 37.8°C). Waterbed (56.5%) had a higher (P < 0.05) lying percentage during cold temperature 1 to 20°F (-17.2 to -6.7°C) than rubber mat (29.8%) and concrete (20.7%), and lower (31.0%) lying percentage during warm temperature 81 to 100°F (27.2 to 37.8°C). Mean percentage lying, across all stall bases, was low during warm temperature 81 to 100°F (27.2 to 37.8°C). Some form of heat abatement may be needed in the facility during warm weather. Future research should be conducted to determine how the use of fans and sprinklers affects stall use.
Figure 5
shows the percentage of stalls with cows lying on the LowSD side for stall base by temperature (°F) interval, which had similar results; however, MATR2 had a slightly higher lying percentage (25.4%) during warm temperature than MATR1 (22.8%).
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Figure 7
shows the percentage of stalls occupied on the LowSD side for stall base by temperature (°F) interval, which showed similar trends; however, MATR2 (47.0%) had a higher (P < 0.05) stall occupied percentage than MATR1 (35.1%) during warm temperature 81 to 100°F (27.2 to 37.8°C) and was similar to MATR1 during cold temperature 1 to 20°F (-17.2 to -6.7°C). The waterbed based stalls were higher (P < 0.05) than rubber mat across all temperature intervals. Concrete was lower during cold temperature 1 to 20°F (-17.2 to -6.7°C) and during mild to warm temperature 41 to 100°F (5.0 to 37.8°C). Cows may prefer a cushioned stall base with a cooler surface during warm temperature.
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Stall use percentages by WDIST showed that cows preferred stalls the closest to and farthest from water (Table 5
). This confusing result can be explained by the significant WDIST x stall base interaction. Sand and MATR1 based stalls were preferred by cows and the 100%SD side had sand based stalls the closest and MATR1 based stalls the farthest from water. Results from the LowSD side showed a similar trend and corresponds to the location of the MATR1 type stalls.
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An analysis of only Wednesday observation data was done to determine if one-day-a-week observation of stall occupancy was sufficient in categorizing stall use versus observing every day for a 9-mo period. Results were similar to those found in Table 1
with some loss in significant differences between stall bases due to fewer observations. It was determined that one-day-a-week analysis would be adequate in determining stall occupancy as a measure of cow preference and comfort.
| CONCLUSIONS |
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Some stall base types were consistently inferior to others. Lying percentages for concrete and rubber mat were always below the average lying percentages. MATR1 based stalls consistently ranked higher than MATR2 for lying and stall occupied percentages, which indicates not all mattresses are equally desirable to cows and making general statements about "mattresses" may be misleading.
The length of time cows are exposed to stall bases affects lying and occupied percentages. The waterbed based stalls required a longer adaptation time whereas use of rubber mat based stalls decreased over time. Cows preferred the exterior row on both sides. Cows may prefer to lie in and occupy stalls near better-ventilated areas. The NOTEND stalls were used more than END stalls. Along with the stall base x STLLOC interaction, this suggests there was something different about the END stalls that affected cow preference. The results of this study yielded conflicting and confusing results about the effect of distance-to-water on stall usage. WDIST was a significant predictor of stall use, but the analysis suggested cows preferred stalls closest to and farthest from water. The significant stall base x WDIST interaction suggests the results to be an artifact of the random selecting of locations.
Temperature appears to affect cows preference for a particular stall base. Lying percentages were higher for MATR1 stalls during hot and cold temperatures, but were higher for sand during moderate temperature conditions. Mattresses may provide insulation from the concrete during cold temperatures while sand may be too cold. As stocking density increased, cow position percentages (lying and stall occupied) increased, suggesting stocking density should be considered when evaluating stall usage. The percentage of stalls with cows lying or were occupied was higher in the morning than the afternoon or night and the highest levels were observed in the early morning before milking and feeding activity began.
Based on the results of this study, the following guidelines are suggested when evaluating the cow comfort of stalls in a freestall barn: If possible, observe the stall occupancy early in the morning before feeding or milking activity has begun. Count the number of stalls with a cow lying and stalls occupied. If the barn has a 100% stocking rate, then a 55 to 65% lying percentage and an 80 to 85% occupancy percentage would be considered very good. As stocking rate changes, expect these percentages to change about proportionately to the stocking rate. If observations are taken later in the day, expect these values to drop by 10 to 20 percentage points.
Further research would lead to a better understanding of how stall location affects cow preference for different stall bases. The interaction with other factors, such as stall base, temperature, or WDIST should be investigated further. Percentage of cows lying and occupying stalls, time of day, temperature, and stocking density should be considered in the development and testing of an alternative index to measure the relative cow comfort of different stall bases.
| ACKNOWLEDGEMENTS |
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Received for publication May 14, 2002. Accepted for publication December 4, 2002.
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