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J. Dairy Sci. 2009. 92:990-995. doi:10.3168/jds.2008-1216
© 2009 American Dairy Science Association ®
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Short communication: Evaluation of an on-farm test to estimate somatic cell count

A. C. O. Rodrigues*, L. D. Cassoli*, P. F. Machado* and P. L. Ruegg{dagger},1

* Clinica do Leite, Department of Animal Science, University of Sao Paulo, Piracicaba 13418, Brazil
{dagger} Department of Dairy Science, University of Wisconsin, Madison 53706

1 Corresponding author: plruegg{at}wisc.edu

The objective of this study was to compare the results of an on-farm test, named Somaticell, with results of electronic cell counting and for milk somatic cell count (SCC) among readers. The Somaticell test correctly determined the SCC in fresh quarter milk samples. Correlation between Somaticell and electronic enumeration of somatic cells was 0.92 and {kappa} coefficient 0.82. Using a threshold of 205,000 cells/mL, the sensitivity and specificity for determination of intramammary infections were 91.3 and 96.0%, respectively. The SCC was greater for milk samples from which major mastitis pathogens were recovered. Minor variation among readers was observed and most likely associated with the mixing procedure. However, the final analysis indicated that this variation was not significant and did not affect the amount of samples classified as having subclinical mastitis. The on-farm test evaluated in this study showed adequate capacity of determining SCC on quarter milk samples and may be considered as an alternative for on-farm detection of subclinical mastitis.

Key Words: somatic cell count • on-farm test • subclinical mastitis






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