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J. Dairy Sci. 2009. 92:773-776. doi:10.3168/jds.2008-1266
© 2009 American Dairy Science Association ®
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Technical note: A new and cost-effective method for detection of the bovine acyl-CoA:diacylglycerol acyltransferase 1 K232A polymorphism in cattle

R. S. Steinberg*, L. Pereira*, G. A. Lacorte*, M. G. C. D. Peixoto{dagger}, R. S. Verneque{dagger}, R. L. Teodoro{dagger}, M. A. Machado{dagger}, C. G. Fonseca* and M. R. S. Carvalho*,1

* Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, 31-270-901, Brazil
{dagger} Embrapa Gado de Leite, Juiz de Fora, 36038-330, Brazil

1 Corresponding author: mraquel{at}icb.ufmg.br

A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection.

Key Words: DGAT1 • polymerase chain reaction-restriction fragment length polymorphism • tetra-primer amplification refractory mutation system-polymerase chain reaction • marker-assisted selection






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