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J. Dairy Sci. 2009. 92:6197-6201. doi:10.3168/jds.2008-1519
© 2009 American Dairy Science Association ®
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Short communication: Polymorphism of casein cluster genes in Czech local goat breeds

Z. Sztankóová*, V. Mátlová*, J. Kyselová*,1, O. M. Jandurová{dagger}, J. Ríha{ddagger} and C. Senese§

* Institute of Animal Science, 104 00 Prague, Czech Republic
{dagger} Research Institute of Crop Production, Research Station of Viticulture, 267 18 Karlstejn, Czech Republic
{ddagger} Mendel University of Agriculture and Forestry, Agronomy Faculty, 613 00 Brno, Czech Republic
§ Dipartimento di Scienze delle Produzioni Animali, Università degli Studi della Basilicata, 85100 Potenza, Italy

1 Corresponding author: kyselova.jitka{at}vuzv.cz

The 4 casein loci were evaluated as haplotypes in 2 dairy goat breeds kept in the Czech Republic. Analysis of 41 White Shorthaired (WSH) trio families and 44 Brown Shorthaired (BSH) trio families revealed 14 and 20 haplotypes, respectively. Various genomic techniques were used to type the casein loci. Twenty-two different combinations of these alleles (casein haplotypes, in the order CSN1S1-CSN2-CSN1S2-CSN3) were found. Only 5 haplotypes in the WSH breed and 6 haplotypes in the BSH breed occurred at frequencies >0.05. For the WSH breed, the most common haplotype was FCFB (0.260), whereas for the BSH breed, the most common haplotype was FCFA (0.217). The information on the haplotype variability in both breeds could be used in breeding programs aimed at preserving biodiversity or selecting animals for specific protein production and cheesemaking.

Key Words: goat • casein haplotype • genetic polymorphism






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