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J. Dairy Sci. 2009. 92:95-99. doi:10.3168/jds.2008-1361
© 2009 American Dairy Science Association ®
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Technical note: Characterizing individual milk fat globules with holographic video microscopy

F. C. Cheong, K. Xiao and D. G. Grier1

Department of Physics and Center for Soft Matter Research, New York University, New York 10003

1 Corresponding author: david.grier{at}nyu.edu

We used in-line holography to create images of individual milk fat globules in diluted samples of milk. Analyzing these images with the exact Lorenz-Mie light scattering theory then yields the droplets’ radii with nanometer resolution and their refractive indexes to within one part in a thousand. This procedure rapidly and directly characterizes both the quantity and quality of fat in milk.

Key Words: globule • holography • milk fat






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