HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Interpretive Summary Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tsuda, H. Articles by Miyamoto, T. Search for Related Content PubMed PubMed Citation Articles by Tsuda, H. Articles by Miyamoto, T.

Binding of Mutagens to Exopolysaccharide Produced by Lactobacillus plantarum Mutant Strain 301102S

H. Tsuda^*, K. Hara and T. Miyamoto^*,1

^* Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushimanaka, Okayama 700-8530, Japan
Shikoku Nyugyou Co. Ltd., 955-1 Minamikata, Touon 791-0397, Japan

¹ Corresponding author: tmnono{at}cc.okayama-u.ac.jp

Exopolysaccharide (EPS) was produced by Lactobacillus plantarum 301102 on exposure to the mutagenic action of acridine orange and novobiocin. The biological characteristics of this mutant strain 301102S were the same as those of the parent strain, but fermented milk prepared with the mutant strain showed antimutagenic activity on 3-amino-1,4-dimethyl-5H-pyrido indole. Only EPS-bound cells of strain 301102S showed binding ability to mutagens such as heterocyclic amines, and the mutagens were inactivated by binding to EPS. The binding ability was affected by pH; the greatest percentage binding was noted at pH 8.0. Addition of Mg²⁺ and sodium dodecyl sulfate, but not oxgall, inhibited the binding ability. Therefore, the binding mechanism of the EPS may consist of ion-exchange and hydrophobic bonds, and the EPS would bind mutagens in the intestine.

Key Words: exopolysaccharide • Lactobacillus plantarum • probiotic • antimutagenic activity