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J. Dairy Sci. 2008. 91:2591-2594. doi:10.3168/jds.2007-0759
© 2008 American Dairy Science Association ®
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Short Communication: Immunity Gene pedB Enhances Production of Pediocin PA-1 in Naturally Resistant Lactococcus lactis Strains

J. L. Arqués*,1, J. M. Rodríguez{dagger}, M. J. Gasson* and N. Horn*

* Commensals and Microflora, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom
{dagger} Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain

1 Corresponding author: juan.arques{at}bbsrc.ac.uk

Heterologous production of the antilisterial bacteriocin pediocin PA-1 in lactococci is an attractive objective to increase the safety of dairy products. In a previous paper, we developed a system for the heterologous production of the bacteriocin pediocin PA-1 in pediocin-resistant lactococcal hosts through a leader exchange strategy. The system was based on 3 genes, 1 encoding the fusion between the lactococcin A leader and propediocin PA-1, and the other 2 encoding the lactococcin A secretion machinery. In this study, we investigated whether the addition of the pediocin PA-1 immunity gene (pedB) to this system has any effect on pediocin production. Introduction of the plasmid(s) carrying the genes described above into nisinproducing and non-nisinproducing lactococcal hosts led to a significant increase in the production of pediocin compared with the equivalent pedB-devoid systems. In addition, we obtained a nisin-producing strain with the ability to secrete pediocin PA-1 at a level equivalent to that of the parental strain Pediococcus acidilactici 347, which represents a notable improvement over our previous systems.

Key Words: Lactococcus lactis • bacteriocin • immunity • heterologous production






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