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Purification and Identification of Bovine Cheese Whey Fatty Acids Exhibiting In Vitro Antifungal Activity

M. Clément^*,1, J. Tremblay^*, M. Lange^,2, J. Thibodeau^* and P. Belhumeur^*

^* Département de microbiologie et immunologie, Université de Montréal, C.P. 6128, succ. Centreville, Montréal, Québec, H3C 3J7, Canada
Saputo Inc., 6869 Métropolitain Est St-Léonard, Montreal, Québec, H1P 1X8, Canada

¹ Corresponding author: martin.clement{at}umontreal.ca

Milk lipids contain several bioactive factors exhibiting antimicrobial activity against bacteria, viruses, and fungi. In the present study, we demonstrate that free fatty acids (FFA) derived from the saponification of bovine whey cream lipids are active in vitro at inhibiting the germination of Candida albicans, a morphological transition associated with pathogenicity. This activity was found to be significantly increased when bovine FFA were enriched in non-straight-chain FFA. At low cell density, this non-straight-chain FFA-enriched fraction was also found to inhibit in a dose-dependant manner the growth of both developmental forms of C. albicans as well as the growth of Aspergillus fumigatus. Using an assay-guided fractionation, the main components responsible for these activities were isolated. On the basis of mass spectroscopic and gas chromatographic analysis, antifungal compounds were identified as capric acid (C10:0), lauroleic acid (C12:1), 11-methyldodecanoic acid (iso-C13:0), myristoleic acid (C14:1n-5), and -linolenic acid (C18:3n-6). The most potent compound was -linolenic acid, with minimal inhibitory concentration values of 5.4 mg/L for C. albicans and 1.3 mg/L for A. fumigatus, in standardized conditions. The results of this study indicate that bovine whey contains bioactive fatty acids exhibiting antifungal activity in vitro against 2 important human fungal pathogens.

Key Words: whey • free fatty acid • Candida • Aspergillus • antifungal