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Short Communication: Characterization of a -Casein Genetic Variant in the Chinese Yak, Bos grunniens

W. L. Bai^*,,1, R. H. Yin^*, S. J. Zhao, Y. C. Zheng, J. C. Zhong and Z. H. Zhao

^* Animal Science and Veterinary Medicine College, Shenyang Agricultural University, Shenyang 110161, China
Animal Science and Veterinary Medicine College, Jilin University, Changchun 130062, China
Animal Science Research Academy of Sichuan Province, Chengdu 610066, China
Life Science and Technology College, Southwest University for Nationalities, Chengdu 610041, China

¹ Corresponding author: wlb7411{at}163.com

A PCR-single strand conformation polymorphism protocol has been developed for rapid genotyping of the yak -casein gene. A total of 307 yaks from the Tianzhu White, Jiulong, Maiwa, and Datong breeds in China were genotyped at the -casein locus using the protocol developed in the present study. A polymorphism of -casein gene exon 4 has been identified in Tianzhu White breed by evaluating genomic DNA. The polymorphic site consists of a single nucleotide substitution GC at position 362 of the exon 4, resulting in an AA substitution from Arg to Pro at position 121 of the AA sequence and in 2 alleles named, respectively, G and C based on nucleotide 362. The occurrence of allele C in the Tianzhu White breed was high with an allele frequency of 0.15. However, allele C appears to be absent in the yaks from Jiulong, Maiwa, and Datong breeds.

Key Words: genetic polymorphism • yak • -casein

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