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Short Communication: Effects of 2-Hydroxy-4-(Methylthio) Butanoic Acid Isopropyl Ester on Milk Production and Composition of Lactating Holstein Dairy Cows

R. H. Phipps^*,1, C. K. Reynolds^*, D. I. Givens^*, A. K. Jones^*, P.-A. Geraert, E. Devillard and R. Bennett

^* Centre for Dairy Research, School of Agriculture, Policy and Development, University of Reading, RG6 6AR, United Kingdom
Adisseo, Commentry 03600, France

¹ Corresponding author: r.h.phipps{at}reading.ac.uk

Sixteen multiparous Holstein cows were used to determine the effects of 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester (HMBi: 0 vs. 1.26 g/kg of total ration dry matter (DM) and dietary crude protein (CP) concentration [14.7% (low) vs. 16.9% (standard), DM basis] on milk yield and composition using a replicated 4 x 4 Latin square design experiment with 4-wk periods. Cows were fed ad libitum a total mixed ration with a 1:1 forage-to-concentrate ratio (DM basis), and diets provided an estimated 6.71 and 1.86% lysine and methionine, respectively, in metabolizable protein for the low-protein diet and 6.74 and 1.82% in the standard protein diet. Dry matter intake, milk yield, and composition were measured during wk 4 of each period. There were no effects on DM intake, which averaged 24.7 kg/d. There was an interaction between dietary CP and HMBi for milk yield and 3.5% fat-corrected milk (FCM). Feeding HMBi decreased milk and FCM yield when fed with the low-CP diet but did not affect milk or FCM yield when fed with the standard CP diet. Feeding HMBi increased milk protein concentration regardless of diet CP concentration and increased milk protein yield when added to the standard CP diet but not the low-CP diet. The positive effect of HMBi on milk protein yield was only observed at the standard level of dietary CP, suggesting other factors limited the response to HMBi when dietary protein supply was restricted.

Key Words: dairy cow • methionine supplement • milk protein

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