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A Milk Diet Partly Containing Soy Protein Does Not Change Growth but Regulates Jejunal Proteins in Young Goats¹

S. Kuhla^*, P. E. Rudolph, D. Albrecht, U. Schoenhusen^*, R. Zitnan, W. Tomek^#, K. Huber^||, J. Voigt^* and C. C. Metges^*,2

^* Research Unit Nutritional Physiology "Oskar Kellner", and
Research Unit Genetics and Biometry, Research Institute for the Biology of Farm Animals (FBN), D-18196 Dummerstorf, Germany
Institute of Microbiology, Ernst-Moritz-Arndt-University, Greifswald, D-17489 Greifswald, Germany
Institute of Animal Production, Slovak Institute for Agricultural Research Nitra, Division Kosice, SK-04001 Kosice, Slovak Republic
^# Research Unit Reproductive Biology, Research Institute for the Biology of Farm Animals (FBN), D-18196 Dummerstorf, Germany
^|| Department of Physiology, School of Veterinary Medicine, Hannover, D-30173 Hannover, Germany

² Corresponding author: metges{at}fbn-dummerstorf.de

Soy protein is known to alter intestinal function and structure. We determined in young goats whether a diet partly containing soy protein differently affects intestinal morphology and the jejunal and hepatic proteome as compared with a milk diet. Fourteen male 2-wk-old White German dairy goat kids were fed comparable diets based on whole cow’s milk in which 35% of the crude protein was casein (milk protein group; MP) or soy protein supplemented by indispensable AA (SPAA) for 34 d (n = 7/group). Body weight gain and food efficiency were not different. Jejunal and hepatic tissue was collected to determine intestinal morphology by microscopy and protein repertoire by 2-dimensional gel electrophoresis and mass spectrometry. Jejunal crypt depth was reduced and villus height to crypt depth ratio was higher in SPAA than in milk protein. Out of 131 proteins identified, 32 proteins were found to be differently expressed in both groups. In SPAA, down-regulated jejunal proteins were involved in processes related to cytoskeleton generation, protein, lipid, and energy metabolism. Downregulated hepatic proteins were related to glycolysis and Krebs cycle. Thirteen proteins were upregulated in SPAA. Among these, 2 hepatic proteins were related to carbohydrate breakdown. The other 11 jejunal proteins were involved in cytoskeleton assembly, proteolysis, and carbohydrate breakdown. In addition, glutathione-S-transferase was found to be upregulated in the medial jejunum. In conclusion, a SPAA diet as compared with a milk diet was related to changes in jejunal morphology and jejunal proteins relevant for protein turnover, energy metabolism, and cytoskeleton assembly with no apparent impact on animal BW gain.

Key Words: intestinal development • goat kid • proteome analysis • soy protein

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