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J. Dairy Sci. 2007. 90:2997-3001. doi:10.3168/jds.2006-547
© 2007 American Dairy Science Association ®
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Characterization of Genetic Polymorphism of the Bovine Lymphocyte Antigen DRB3.2 Locus in Kankrej Cattle (Bos indicus)

J. D. Behl1, N. K. Verma, R. Behl, M. Mukesh and S. P. S. Ahlawat

National Bureau of Animal Genetic Resources, P.O. Box No. 129, G.T. Bypass Road, Karnal, Haryana, India

1 Corresponding author: jyotsna1970{at}yahoo.com

Bovine lymphocyte antigen DRB 3.2 (BoLA–DRB3.2) gene encodes for the beta chain of the major histocompatibility complex (MHC) class II molecule in cattle, which is a glycoprotein present on the surface of antigen-presenting cells. This locus shows extensive polymorphism in it. The objective of the present study was to genotype the BoLA-DRB3.2 locus in Kankrej cattle (n = 50) by PCR-RFLP. Bovine DNA was isolated from aliquots of whole blood. Primers specific for exon 2 of the bovine lymphocyte antigen (BoLA)-DRB3 gene were used to amplify the region. The 304-bp amplified product of the DRB3 gene was separately digested with restriction endonucleases RsaI, BstYI, and Hae III. Twenty-four BoLA-DRB 3.2 alleles were identified with frequencies ranging from 1 to 22.0%. Twenty-one alleles of the total 24 alleles were similar to those reported earlier; 3 alleles were new and had not been reported previously. The allele BoLA-DRB3.2*34 occurred at the highest frequency of 22% (approx.) in the Kankrej animals studied. Six alleles (BoLA-DRB3.2 *34, *15, *06, *20, *37, and *20) accounted for almost 71% of the total alleles observed to be present in the Kankrej animals. All the new alleles observed were present at frequencies of 1%. The results obtained in the present study demonstrated that the BoLA DRB3.2 locus is highly polymorphic in the Kankrej cattle.

Key Words: bovine lymphocyte antigen • Bos indicus • polymorphism






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