| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Dairy Science, University of Wisconsin, Madison 53706
1 Corresponding author: hkhatib{at}wisc.edu
The uterine milk proteins (UTMP) are the major proteins secreted by the endometrium, primarily under the control of progesterone. Specific functions of UTMP are poorly understood, but may include protease inhibition, nutrition of the conceptus, growth control, and suppression of the maternal immune system. The uterine milk protein gene (UTMP) was chosen for this study because of its possible roles in health traits and results of previous studies on the association of the UTMP region with milk production and productive life in dairy cattle. Expression of UTMP was examined in 198 bovine tissues obtained from 10 fetuses and 17 cows. Transcripts of UTMP were found in all cotyledon tissues examined and to a lesser extent in ovary, pituitary, and spleen tissues obtained from fetuses. The UTMP gene was predominantly expressed in endometrium (17/17), ovary (15/16), and caruncle (12/12) tissues obtained from cows. The predominant expression of UTMP in reproductive tissues is consistent with an important role of this gene in reproductive success. To investigate the association between UTMP and production traits in cattle, we identified 2 synonymous single nucleotide polymorphisms (SNP) at positions 1179 (A/G) and 1296 (A/G) using the pooled DNA sequencing approach. The DNA was extracted from 28 Holstein sires and their 1,362 sons obtained from the Cooperative Dairy DNA Repository and from 913 cows from the University of Wisconsin resource population. Single nucleotide polymorphism 1296 was associated with a significant increase in productive life in both populations. This finding is similar to results previously obtained for the protease inhibitor gene, which is near UTMP and was also found to be associated with productive life in dairy cattle. Differential allelic expression of UTMP was observed in reproductive tissues obtained from 9 heterozygous individuals. The differential allelic expression observed in this study is consistent with other studies showing a correlation between allelic variation in gene expression and phenotypic variability.
Key Words: uterine milk protein gene • differential allelic expression • candidate gene • productive life
This article has been cited by other articles:
|
|
 |
|
  H. Khatib, W. Huang, X. Wang, A. H. Tran, A. B. Bindrim, V. Schutzkus, R. L. Monson, and B. S. Yandell Single gene and gene interaction effects on fertilization and embryonic survival rates in cattle J Dairy Sci, May 1, 2009; 92(5): 2238 - 2247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Khatib, C. Maltecca, R. L. Monson, V. Schutzkus, X. Wang, and J. J. Rutledge The fibroblast growth factor 2 gene is associated with embryonic mortality in cattle J Anim Sci, September 1, 2008; 86(9): 2063 - 2067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, C. Maltecca, R. Tal-Stein, E. Lipkin, and H. Khatib Association of Bovine Fibroblast Growth Factor 2 (FGF2) Gene with Milk Fat and Productive Life: An Example of the Ability of the Candidate Pathway Strategy to Identify Quantitative Trait Genes J Dairy Sci, June 1, 2008; 91(6): 2475 - 2480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Khatib, R. L. Monson, V. Schutzkus, D. M. Kohl, G. J. M. Rosa, and J. J. Rutledge Mutations in the STAT5A Gene Are Associated with Embryonic Survival and Milk Composition in Cattle J Dairy Sci, February 1, 2008; 91(2): 784 - 793. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
