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Influence of Calcium Salt Supplementation on Calcium Equilibrium in Skim Milk During pH Cycle

L. S. Canabady-Rochelle^*,1, C. Sanchez^*, M. Mellema, A. Bot, S. Desobry^* and S. Banon^*

^* Nancy-University Laboratoire de Sciences et Gènie Alimentaires, Ecole Nationale Supérieure d’Agronomie et des Industries Alimentaires, 54 500 Vanduvre-Lés-Nancy, France
Unilever Research and Development Vlaardingen, Olivier van Noortlaan 12, 3133 TG Vlaardingen, the Netherlands

¹ Corresponding author: Laetitia.Canabady{at}ensaia.inpl-nancy.fr

Calcium is a mineral essential for humans, especially for bone constitution. Yet most of the worldwide population does not satisfy their Ca needs. Hence, Ca supplementation is of major importance, even in western countries where some specific populations at risk do not satisfy the recommended daily intake of Ca. More than 70% of dietary Ca comes from dairy products. Calcium supplementation of naturally Ca-rich sources such as skim milk is then of special interest. To our knowledge, few data are available concerning milk Ca (MC) supplementation of milk, particularly when followed by pH cycle. In this paper, MC supplementation is studied and compared with Ca chloride (CC) supplementation as a well-known source of Ca. The effect of Ca salt supplementation followed by pH cycle was studied in reconstituted skim milk. Calcium supplementation was carried out with CC and MC at 25 mmol of Ca/kg of skim milk. Ionized Ca concentration and turbidity variations were followed in situ by Ca ion selective electrode and turbidimetry using light reflection. From normalized data on ionized Ca concentration and turbidity vs. pH, it appeared that hysteresis areas were smaller for CC-supplemented milk, whereas unsupplemented milk and MC-supplemented milk behaved similarly. For these 3 dairy systems, pH cycles to pH 5.0 led to a larger hysteresis area than pH cycles to pH 5.5. The shrinkage of the hysteresis area could be interpreted as a reinforcement of casein micelles with Ca ions over the pH cycle.

Key Words: calcium supplementation • pH cycle • skim milk