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Short Communication: Cellular Localization of Haptoglobin mRNA in the Experimentally Infected Bovine Mammary Gland

M. A. Thielen^*, M. Mielenz^*, S. Hiss^*, H. Zerbe, W. Petzl, H.-J. Schuberth, H.-M. Seyfert and H. Sauerwein^*,1

^* Institute of Animal Science, Physiology & Hygiene Group, University of Bonn, 53115 Bonn, Germany
Clinic for Ruminants, University of Munich, 85764 Oberschleissheim, Germany
Institute for Immunology, University of Veterinary Medicine, 30173 Hannover, Germany
Molecular Biology Research Division, Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany

¹ Corresponding author: sauerwein{at}uni-bonn.de

Expression of haptoglobin (Hp) mRNA, one of the major acute phase proteins in cattle, was demonstrated in homogenates of the bovine mammary gland. The aim of this study was to localize Hp mRNA expression within the udder at the cellular level during the first 24 h of infection with Escherichia coli. For this purpose, 3 quarters of 3 cows were subsequently inoculated with E. coli at 6, 12, and 24 h preslaughter; the fourth quarter received saline at 24 h preslaughter as a control. After slaughter, tissue samples of each quarter were collected for analyses by in situ hybridization and real-time reverse-transcription PCR. Haptoglobin mRNA expression was allocated to the alveolar epithelium of the mammary gland. Quantification of Hp-positive cells in in situ hybridization of Hp mRNA from tissue homogenates and of Hp protein in milk confirmed increasing concentrations within 24 h of infection.

Key Words: haptoglobin • mRNA expression • dairy cow • mastitis

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