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Performance of Blue-Yellow Screening Test for Antimicrobial Detection in Ovine Milk

B. Linage^*, C. Gonzalo^*,1, J. A. Carriedo^*, J. A. Asensio, M. A. Blanco, L. F. De La Fuente^* and F. San Primitivo^*

^* Departamento de Producción Animal, Facultad de Veterinaria, Universidad de León, 24071 León, Spain
Consorcio de Promoción del Ovino, 49630-Villalpando, Zamora, Spain

¹ Corresponding author: c.gonzalo{at}unileon.es

Drug residues in milk are important because of public health and industrial implications. The detection limits of 25 antimicrobial agents were determined by the blue-yellow screening method in ovine milk. For each drug, 8 concentrations were tested on 20 ovine milk samples from individual ewes in midlactation. Detection limits determined by means of logistic regression were below European Union maximum residue limits (EU-MRL) for penicillin G (3 to 4 µg/kg), ceftiofur (96 to 107 µg/kg), framycetin (720 to 781 µg/kg), neomycin (915 to 1,084 µg/kg), and tylosin (44 to 51 µg/kg). Detection limits for ampicillin (5 to 6 µg/kg), cloxacillin (33 to 42 µg/kg), cefoperazone (73 to 82 µg/kg), cefalexin (160 to 202 µg/kg), gentamycin (355 to 382 µg/kg), streptomycin (3,063 to 3,593 µg/kg), tilmicosin (109 to 131 µg/kg), erythromycin (444 to 522 µg/kg), spyramicin (1,106 to 1,346 µg/kg), sulfadimethoxine (101 to 119 µg/kg), sulfathiazole (122 to 151 µg/kg), sulfamethazine (309 to 328 µg/kg), sulfanilamide (1,750 to 2,674 µg/kg), tetracycline (233 to 257 µg/kg), oxytetracycline (398 to 501 µg/kg), doxycycline (323 to 419 µg/kg), chlortetracycline (3,331 to 3,989 µg/kg), danofloxacin (4.7 to 5.5 mg/kg), enrofloxacin (41 to 46 mg/kg), and flumequin (63 to 71 mg/kg) were higher than the EU-MRL. Although the blue-yellow method showed improved sensitivity compared with other tests studied in ovine milk, the performance of screening methods for detecting antimicrobial agents in milk of this species should be improved.

Key Words: ovine milk • screening test • detection limit • antimicrobial residue