HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Interpretive Summary Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Drillich, M. Articles by Heuwieser, W. Search for Related Content PubMed PubMed Citation Articles by Drillich, M. Articles by Heuwieser, W.

Ceftiofur Derivatives in Serum, Uterine Tissues, Cotyledons, and Lochia after Fetal Membrane Retention

M. Drillich^*,1, S. Arlt^*, S. Kersting^*, A. A. Bergwerff, P. Scherpenisse and W. Heuwieser^*

^* Clinic for Reproduction, Faculty of Veterinary Medicine, Free University of Berlin, Königsweg 65, D-14163 Berlin, Germany
Division of Public Health and Food Safety, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.175, NL-3508 TD, Utrecht, the Netherlands

¹ Corresponding author: author{at}bestandsbetreuung.de

The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and lochia during 72 h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12 h were studied. All cows received 3 consecutive injections (C1 to C3; 24 h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and lochia were collected immediately before each injection (0 h) and again at 4, 12, and 24 h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5 g) was collected by using Kenny’s biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2 h (serum), 4 h (endometrium), and 12 h (caruncles, cotyledons, lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.

Key Words: retained fetal membrane • ceftiofur

This article has been cited by other articles:

				M. Drillich, N. Klever, and W. Heuwieser Comparison of Two Management Strategies for Retained Fetal Membranes on Small Dairy Farms in Germany J Dairy Sci, September 1, 2007; 90(9): 4275 - 4281. [Abstract] [Full Text] [PDF]