HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Interpretive Summary Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miguel, M. Articles by Aleixandre, M. A. Search for Related Content PubMed PubMed Citation Articles by Miguel, M. Articles by Aleixandre, M. A.

Antihypertensive Effect of Peptides Obtained from Enterococcus faecalis-Fermented Milk in Rats

M. Miguel^*, I. Recio^*, M. Ramos^*, M. A. Delgado and M. A. Aleixandre^,1

^* Instituto de Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas, Juan de la Cierva 3, 28006 Madrid, Spain
Grupo Leche Pascual, Ctra. Palencia s/n, 09400 Aranda de Duero, Burgos, Spain
Instituto de Farmacología y Toxicología (CSIC), Facultad de Medicina, Universidad Complutense, Avda. Complutense s/n 28040 Madrid, Spain

¹ Corresponding author: amaya{at}med.ucm.es

Previous studies have demonstrated that milk fermented with Enterococcus faecalis decreases the systolic blood pressure (SBP) and the diastolic blood pressure (DBP) of spontaneously hypertensive rats. In this study, we evaluated the antihypertensive activity of the following peptide sequences: LHLPLP, LHLPLPL, LVYPFPGPIPNSLPQNIPP, VLGPVRGPFP, and VRGPFPIIV. These peptides isolated from E. faecalis-fermented milk showed in vitro angiotensin I-converting enzyme-inhibitory activity. Because the most potent angiotensin I-converting enzyme-inhibitory sequences were LHLPLP and LVYPFPGPIPNSLPQ-NIPP, we administered different doses of these peptides to spontaneously hypertensive rats. High doses of the remaining sequences were also administered to these animals. Water served as a negative control and captopril as a positive control. All products were administered orally. The SBP and DBP were measured before administration and also at 2, 4, 6, 8, and 24 h after administration. Before administration of the different products, spontaneously hypertensive rats showed SBP and DBP values of 218 ± 2.5 and 157 ± 5.9 mmHg, respectively (n = 30). The sequences LHLPLP, LVYPF-PGPIPNSLPQNIPP, VLGPVRGPFP, and VRGPFPIIV caused clear and significant decreases in SBP, DBP, or both in the animals. In particular, the antihypertensive effect could be clearly established when 2 or 3 mg/kg of LHLPLP was administered. These 2 doses of LHLPLP showed similar antihypertensive properties. Four hours after administration of captopril or the highest doses of the different peptides, the decreases in the SBP and the DBP (mmHg) were as follows: captopril (SBP = 52 ± 5.8, DBP = 38.8 ± 3.8), 3 mg/kg of LHLPLP (SBP = 25.3 ± 8.2, DBP = 29.5 ± 7.6), 6 mg/kg of LVYPFPGPIP-NSLPQNIPP (SBP = 14.9 ± 3.7, DBP = 8.7 ± 4.4), 10 mg/kg of LHLPLPL (SBP = 7.7 ± 4.1, DBP = 9.4 ± 3.1), 10 mg/kg of VLGPVRGPFP (SBP = 16.2 ± 5.8, DBP = 21.64 ± 3.2), and 10 mg/kg of VRGPFPIIV (SBP = 16.05 ± 2.74, DBP = 9.19 ± 3.49). The results obtained suggest that the sequences LHLPLP, LVYPFPGPIPNSLPQ-NIPP, VLGPVRGPFP, and VRGPFPIIV could be responsible, at least in part, for the antihypertensive properties described for E. faecalis-fermented milk.

Key Words: angiotensin I-converting enzyme inhibitory peptide • antihypertensive peptide • arterial blood pressure • spontaneously hypertensive rat

This article has been cited by other articles:

				M. Hayes, C. Stanton, H. Slattery, O. O'Sullivan, C. Hill, G. F. Fitzgerald, and R. P. Ross Casein Fermentate of Lactobacillus animalis DPC6134 Contains a Range of Novel Propeptide Angiotensin-Converting Enzyme Inhibitors Appl. Envir. Microbiol., July 15, 2007; 73(14): 4658 - 4667. [Abstract] [Full Text] [PDF]