HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Interpretive Summary Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Geerlings, A. Articles by Duarte, J. Search for Related Content PubMed PubMed Citation Articles by Geerlings, A. Articles by Duarte, J.

Identification and Characterization of Novel Angiotensin-Converting Enzyme Inhibitors Obtained from Goat Milk

A. Geerlings^*,1,2, I. C. Villar^,2, F. Hidalgo Zarco^*, M. Sánchez, R. Vera, A. Zafra Gomez^*, J. Boza^* and J. Duarte

^* Department of Biomedicine, Puleva Biotech, Camino de Purchil 66, 18004 Granada, Spain
Department of Pharmacology, School of Pharmacy, University of Granada, Granada, Spain

¹ Corresponding author: ageerlings{at}pulevabiotech.es

The aim of the present study was to identify and characterize new inhibitory peptides of angiotensin I-converting enzyme (ACE) from goat milk and to analyze the effect of long-term intake of a goat milk hydrolysate-supplemented (GP-hyd) diet on the development of hypertension in spontaneously hypertensive rats (SHR). Three new inhibitory peptides for ACE (TGPIPN, SLPQ, and SQPK) were isolated. The inhibitory concentration 50% (IC₅₀) values of individual peptides were 316, 330, and 354 µmol/L, respectively. Only TGPIPN was found to pass intact a monolayer of Caco-2 cells in small amounts. The SHR fed for 12 wk a diet (GP-hyd) enriched in a hydrolysate containing these peptides (estimated intake of TGPIPN was 230 mg/kg per d) showed lower (approximately 15 mmHg) systolic blood pressure than animals fed a control diet. The ACE activities in the aorta, left ventricle, and kidney were significantly decreased in the GP-hyd group compared with those of the control group and were similar to those found in SHR fed captopril (130 mg/kg per d). Impaired endothelium-dependent relaxation to acetylcholine by aortic rings from SHR was improved in those fed the GP-hyd diet. The left ventricle weight and kidney weight index were significantly reduced in the GP-hyd group and captopril groups. Moreover, long-term treatment of SHR with a diet enriched in goat milk hydrolysate, or captopril, attenuated the development of hypertension, cardiac and renal hypertrophy, and endothelial dysfunction. These effects might be related to the in vivo inhibitory effects of the hydrolysate on tissue ACE activity.

Key Words: bioactive peptide • angiotensin-converting enzyme inhibitor • goat milk • spontaneously hypertensive rat

This article has been cited by other articles:

				S. Lamothe, G. Robitaille, D. St-Gelais, and M. Britten Short Communication: Extraction of {beta}-Casein from Goat Milk J Dairy Sci, December 1, 2007; 90(12): 5380 - 5382. [Abstract] [Full Text] [PDF]