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Technical Note: A Rapid Pulsed-Field Gel Electrophoresis Method for Analysis of Bifidobacteria

Department of Food Science, The Pennsylvania State University, University Park 16802

¹ Corresponding author: rfr3{at}psu.edu

Pulsed-field gel electrophoresis (PFGE) is a widely used and highly discriminatory molecular typing method that has been applied to bifidobacteria. However, published PFGE protocols used with bifidobacteria require between 5 and 7 d to complete. A rapid PFGE method was developed that can be completed within 24 h.

Key Words: pulsed-field gel electrophoresis • Bifidobacterium

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