JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Interpretive Summary
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Soliva-Fortuny, R.
Right arrow Articles by Martín-Belloso, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Soliva-Fortuny, R.
Right arrow Articles by Martín-Belloso, O.
J. Dairy Sci. 89:4096-4104
© American Dairy Science Association, 2006.

Modeling High-Intensity Pulsed Electric Field Inactivation of a Lipase from Pseudomonas fluorescens

R. Soliva-Fortuny, S. Bendicho-Porta and O. Martín-Belloso1

Departament de Tecnologia d’Aliments, TPV-CeRTA, Universitat de Lleida, Alcalde Rovira Roure 191, 25198, Lleida, Spain

1 Corresponding author: omartin{at}tecal.udl.es

The inactivation kinetics of a lipase from Pseudomonas fluorescens (EC 3.1.1.3.) were studied in a simulated skim milk ultrafiltrate treated with high-intensity pulsed electric fields. Samples were subjected to electric field intensities ranging from 16.4 to 27.4 kV/cm for up to 314.5 µs, thus achieving a maximum inactivation of 62.1%. The suitability of describing experimental data using mechanistic first-order kinetics and an empirical model based on the Weibull distribution function is discussed. In addition, different mathematical expressions relating the residual activity values to field strength and treatment time are supplied. A first-order fractional conversion model predicted residual activity with good accuracy (Af = 1.018). A mechanistic insight of the model kinetics was that experimental values were the consequence of different structural organizations of the enzyme, with uneven resistance to the pulsed electric field treatments. The Weibull model was also useful in predicting the energy density necessary to achieve lipase inactivation.

Key Words: high-intensity pulsed electric field • lipase • inactivation kinetic • simulated skim milk ultrafiltrate






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Dairy Science Association ®.