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J. Dairy Sci. 89:3826-3832
© American Dairy Science Association, 2006.

Conjugated Linoleic Acid Reduces Phorbol Ester-Induced Prostaglandin F2{alpha} Production by Bovine Endometrial Cells1

C. Rodriguez-Sallaberry, C. Caldari-Torres, E. S. Greene and L. Badinga2

Department of Animal Sciences University of Florida, Gainesville 32611

2 Corresponding author: Badinga{at}animal.ufl.edu

Recent interest in conjugated linoleic acid (CLA) research stems from the well-documented anticarcinogenic, antiatherogenic, antidiabetic, and antiobesity properties of CLA in animal models. The objective of this study was to examine the effects of 2 CLA isomers (cis-9,trans-11 and trans-10,cis-12) on phorbol 12,13-dibutyrate (PDBu)-induced PGF2{alpha} production in cultured bovine endometrial (BEND) cells. Confluent BEND cells were incubated in the absence (control) or presence of 100 µM each of linoleic acid, cis-9,trans-11 CLA, or trans-10,cis-12 CLA for 24 h. After incubation, cells were rinsed and then stimulated with PDBu (100 ng/mL) for 6 h. Compared with untreated cells, PDBu stimulated PGF2{alpha} secretion (+25-fold) within 6 h. The increases in PGF2{alpha} secretion were paralleled by signifi-cant induction of prostaglandin endoperoxide synthase-2 (PGHS-2) mRNA (+63-fold) and protein (+1.6-fold) expression. In spite of stimulatory effects on PGHS-2 and peroxisome proliferator-activated receptor {delta} (PPAR{delta}) mRNA responses, CLA greatly decreased PGF2{alpha} production by PDBu-stimulated BEND cells. There was no evidence for PDBu or CLA modulation of PPAR{delta} protein synthesis in cultured BEND cells. Results indicated that CLA modulation of PGF2{alpha} production by BEND cells was not mediated through PGHS-2 or PPAR{delta} gene repression.

Key Words: conjugated linoleic acid • uterus • prostaglandin F2{alpha} • cow




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[Abstract] [Full Text] [PDF]


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