HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Interpretive Summary Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rabello, R. F. Articles by Castro, A. C. D. Search for Related Content PubMed PubMed Citation Articles by Rabello, R. F. Articles by Castro, A. C. D.

Characterization of Staphylococcus aureus Isolates Recovered from Bovine Mastitis in Rio de Janeiro, Brazil

Instituto de Microbiologia, Universidade Federal do Rio de Janeiro CCS—Bloco I—Cidade Universitária, Rio de Janeiro, RJ. 21941-590, Brazil

Corresponding author: Angela Christina Dias de Castro; e-mail: acastro{at}micro.ufrj.br.

Phenotypic characteristics, antimicrobial susceptibility, and genetic relationships were analyzed in 107 Staphylococcus aureus isolates recovered from cows with subclinical mastitis in Southeastern Brazil. Thirteen different biochemical patterns were detected among isolates. A predominant pattern represented by about 54% of the isolates was distributed among several herds. Isolates of distinct phenotypic profiles were also detected within a herd. Susceptibility to ampicillin, cefotaxime, cephalotin, chloramphenicol, erythromycin, gentamycin, kanamycin, nitrofurantoin, norfloxacin, ofloxacin, oxacillin, penicillin, rifampin, sulfamethoxazole-trimethoprim, tetracycline, trimethoprim, and vancomycin, determined by the disk diffusion method, was observed in 44.9% of isolates. On the other hand, 55.1, 7.4, and 2.8% of the strains were resistant to ampicillin/penicillin, tetracycline, and erythromycin, respectively. Genetic diversity was analyzed by pulsed-field gel electrophoresis using SmaI as the restriction enzyme. All isolates could be typed by pulsed-field gel electrophoresis, which identified 16 types and 24 subtypes. Type A and its subtypes comprised 54.2% of all isolates and were recovered from 6 of the 9 herds analyzed. Other types and subtypes were also found in multiple herds. Although multiple types and subtypes were found within a specific herd, a predominant type was frequently observed.

Key Words: Staphylococcus aureus • bovine mastitis • antimicrobial resistance • genetic diversity

Abbreviation key: PFGE = pulsed-field gel electrophoresis

This article has been cited by other articles:

				R. F. Rabello, B. M. Moreira, R. M. M. Lopes, L. M. Teixeira, L. W. Riley, and A. C. D. Castro Multilocus sequence typing of Staphylococcus aureus isolates recovered from cows with mastitis in Brazilian dairy herds J. Med. Microbiol., November 1, 2007; 56(11): 1505 - 1511. [Abstract] [Full Text] [PDF]

				M. Aires-de-Sousa, C. E. S. R. Parente, O. Vieira-da-Motta, I. C. F. Bonna, D. A. Silva, and H. de Lencastre Characterization of Staphylococcus aureus Isolates from Buffalo, Bovine, Ovine, and Caprine Milk Samples Collected in Rio de Janeiro State, Brazil Appl. Envir. Microbiol., June 15, 2007; 73(12): 3845 - 3849. [Abstract] [Full Text] [PDF]

				K. L. Anderson and R. L. Lyman Long-Term Persistence of Specific Genetic Types of Mastitis-Causing Staphylococcus aureus on Three Dairies J Dairy Sci, December 1, 2006; 89(12): 4551 - 4556. [Abstract] [Full Text] [PDF]