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Application of Polymerase Chain Reaction to Detect Adulteration of Sheep’s Milk with Goats’ Milk

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain

Corresponding author: Isabel González Alonso; e-mail: gonzalzi{at}vet.ucm.es.

The polymerase chain reaction has been applied for the specific detection of goats’ milk in sheep’s milk using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of goat-specific primers yielded a 122-bp fragment from goats’ milk DNA, whereas no amplification signal was obtained in sheep’s, cows’, and water buffaloes’ milk DNA. Polymerase chain reaction analysis of raw and heat-treated milk binary mixtures of sheep/goat enabled the specific detection of goats’ milk with a sensitivity threshold of 0.1%. This study demonstrates the usefulness of the proposed polymerase chain reaction assay for authentication of milk products in routine analysis.

Key Words: polymerase chain reaction • goats’ milk • sheep’s milk • 12S rRNA gene