|
|
||||||||
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Corresponding author: Isabel González Alonso; e-mail: gonzalzi{at}vet.ucm.es.
The polymerase chain reaction has been applied for the specific detection of goats milk in sheeps milk using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of goat-specific primers yielded a 122-bp fragment from goats milk DNA, whereas no amplification signal was obtained in sheeps, cows, and water buffaloes milk DNA. Polymerase chain reaction analysis of raw and heat-treated milk binary mixtures of sheep/goat enabled the specific detection of goats milk with a sensitivity threshold of 0.1%. This study demonstrates the usefulness of the proposed polymerase chain reaction assay for authentication of milk products in routine analysis.
Key Words: polymerase chain reaction goats milk sheeps milk 12S rRNA gene
This article has been cited by other articles:
![]() |
W. L. Bai, R. H. Yin, S. J. Zhao, Q. L. Dou, J. C. Yang, W. Q. Jiang, Z. H. Zhao, and G. B. Luo Rapid detection of bovine milk in yak milk using a polymerase chain reaction technique J Dairy Sci, April 1, 2009; 92(4): 1354 - 1360. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |