| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi di Milano, 20134 Milano, Italy
2 Fondazione Parco Tecnologico Padano, Centro Ricerche e Studi Agroalimentari, 20090 Segrate (Milano), Italy
3 Dipartimento di Sanità e Benessere Animale, Università di Bari, 70010 Valenzano (Bari), Italy
Corresponding author: Anna Caroli; e-mail: am.caroli{at}veterinaria.uniba.it.
A protocol for the rapid and simultaneous genotyping of A, C, and 0 'CSN2 alleles in goat was developed by single strand conformational polymorphism polymerase chain reaction (SSCP-PCR) technique. Screening the CSN2 variability in 7 goat breeds reared in Italy validated the genotyping test. The SSCP-PCR technique was also suitable for monitoring CSN2 polymorphism. In particular, the discrimination between CSN2*A and CSN2*C is important because the 2 corresponding protein variants cannot be separated by standard typing techniques. The monitoring of CSN2 variability in the goat breeds indicates the predominance of the C allele. In most breeds, CSN2*C occurred with the highest frequency, except in Saanen where CSN2*A and CSN2*C showed similar frequencies. Variant CSN2*C occurred with a frequency of 0.68 (Camosciata), 0.70 (Jonica), 0.71 (Garganica), 0.82 (Maltese), 0.87 (Cilentana), and 0.97 (Orobica). The alignment among the mature CSN2 sequences of different species suggests that CSN2*A is the ancestral allele compared with CSN2*C. Interestingly, the CSN2*A goat variant showed higher frequencies in selected breeds (Saanen and Camosciata).
Key Words: goat • ß-casein • genetic polymorphism • single strand conformational polymorphism
Abbreviation key: CSN2 = ß-casein, IEF = isoelectrofocusing, SSCP = single strand conformational polymorphism
This article has been cited by other articles:
|
|
 |
|
  S. Chessa, D. Rignanese, J. Kupper, G. Pagnacco, G. Erhardt, and A. Caroli Short Communication: The {beta}-Casein (CSN2) Silent Allele C1 Is Highly Spread in Goat Breeds J Dairy Sci, November 1, 2008; 91(11): 4433 - 4436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Sztankoova, J. Kysel'ova, T. Kott, and E. Kottova Technical Note: Detection of the C Allele of {beta}-Casein (CSN2) in Czech Dairy Goat Breeds Using LightCycler Analysis J Dairy Sci, October 1, 2008; 91(10): 4053 - 4057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gigli, D. O. Maizon, V. Riggio, M. T. Sardina, and B. Portolano Short Communication: Casein Haplotype Variability in Sicilian Dairy Goat Breeds J Dairy Sci, September 1, 2008; 91(9): 3687 - 3692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chessa, D. Rignanese, F. Chiatti, A. Radeghieri, C. Gigliotti, and A. Caroli Technical Note: Simultaneous Identification of CSN1S2 A, B, C, and E Alleles in Goats by Polymerase Chain Reaction-Single Strand Conformation Polymorphism J Dairy Sci, March 1, 2008; 91(3): 1214 - 1217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Caroli, F. Chiatti, S. Chessa, D. Rignanese, E. M. Ibeagha-Awemu, and G. Erhardt Characterization of the Casein Gene Complex in West African Goats and Description of a New {alpha}s1-Casein Polymorphism J Dairy Sci, June 1, 2007; 90(6): 2989 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Caroli, F. Chiatti, S. Chessa, D. Rignanese, P. Bolla, and G. Pagnacco Focusing on the goat casein complex. J Dairy Sci, August 1, 2006; 89(8): 3178 - 3187. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
