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Short Communication: Ca²⁺-Adenosine Triphosphatase Protein Expression in the Mammary Gland of Periparturient Cows

Periparturient Diseases of Cattle Research Unit, USDA–ARS, National Animal Disease Center, Ames, IA 50010

Corresponding author: T. A. Reinhardt; e-mail: treinhar{at}nadc.ars.usda.gov.

The objectives of this study were to measure the changes in protein expression of the mammary Ca²⁺-ATPases during the periparturient period and to determine whether Ca²⁺-ATPase protein expression in the mammary gland is related to milk fever (MF) development. Abundance of Ca²⁺-ATPase in mammary tissue and milk fat globule membranes was determined by Western blotting. The secretory pathway Ca²⁺-ATPase was elevated prepartum in mammary tissue from cows that developed MF compared with non-MF cows.

Key Words: Ca²⁺-adenosine triphosphatase • milk fever • milk fat globule membrane • mammary gland

Abbreviation key: MF = milk fever, MFGM = milk fat globule membrane, MG = mammary gland, PMCA = plasma membrane Ca²⁺-ATPase (The number following PMCA and SPCA refers to the specific isoform. The first lowercase letter following the number in PMCA refers to the splice site C splice form, and the next lowercase letter refers to the splice site A splice form). SPCA = secretory pathway Ca²⁺-ATPase

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