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J. Dairy Sci. 88:1399-1403
© American Dairy Science Association, 2005.

Subacute Ruminal Acidosis Induces Ruminal Lipopolysaccharide Endotoxin Release and Triggers an Inflammatory Response

G. N. Gozho, J. C. Plaizier, D. O. Krause, A. D. Kennedy and K. M. Wittenberg

Department of Animal Science, University of Manitoba, Winnipeg, Canada R3T 2N2

Corresponding author: J. C. Plaizier; e-mail: plaizier{at}ms.umanitoba.ca.

Subacute ruminal acidosis (SARA) was induced in 3 rumen fistulated Jersey steers by offering them different combinations of wheat-barley pellets and chopped alfalfa hay. Steers were offered 4, 5, and 6 kg/d of pelleted concentrate and 6, 5, and 4 kg/d of chopped alfalfa hay for diets 1, 2, and 3, respectively, during 5-d treatment periods and were fed chopped alfalfa hay between treatment periods. Inducing SARA increased blood concentrations of haptoglobin and serum amyloid-A. Dry matter intake of concentrate and hay decreased from d 1 to 5 in each period. Subacute ruminal acidosis was induced in all steers during d 4 and 5 when concentrate was fed, with ruminal pH remaining below 5.6 for an average of 187 and 174 min/d on these days. Lipopolysaccharide concentration increased significantly during periods of grain feeding compared with times when only hay was fed. Inducing SARA by feeding wheat-barley pellets activated a systemic inflammatory response in the steers.

Key Words: steers • subacute ruminal acidosis • ruminal pH • acute phase response

Abbreviation key: Hp = haptoglobin, LAL = Limulus amebocyte lysate, SAA = serum amyloid-A, SARA = subacute ruminal acidosis.




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