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Effects of Bovine Somatotropin on Uterine Genes Related to the Prostaglandin Cascade in Lactating Dairy Cows^*

Department of Animal Sciences, University of Florida, Gainesville 32611

Corresponding author: L. Badinga; e-mail: Badinga{at}animal.ufl.edu.

Multiparous Holstein cows, averaging 80 d in milk, were used to examine the effect of exogenous bovine somatotropin (bST) on uterine expression of estrogen receptor (ER), prostaglandin endoperoxide synthase-2 (PGHS-2), and peroxisome proliferator-activated receptor (PPAR). About 12 h before expected ovulation in a synchronization protocol, cows were assigned to receive bST (500 mg, n = 11) or serve as untreated controls (n = 10). Cows that ovulated (n = 9 bST, 8 control) were divided within treatment to be killed on d 3 or 7 postovulation. Samples of intercaruncular endometrial tissue from uterine horns ipsilateral to the corpus luteum were collected and stored at –80°C for subsequent mRNA analyses. Endometrial concentrations of ER and PGHS-2 mRNA transcripts were greater on d 7 than on d 3 of the estrous cycle, but did not differ between treatments. Compared with untreated cows, short-term bST treatment decreased PGHS-2 protein expression at d 7 of the estrous cycle. Concentration of PPAR mRNA transcript in the uterus decreased between d 3 and 7 of the estrous cycle and was negatively correlated with ER and PGHS-2 mRNA concentrations. Short-term administration of bST to lactating dairy cows had minimal effects on uterine genes encoding ER, PGHS-2, and PPAR at d 3 and 7 of the estrous cycle but there may be an inverse relationship between PPAR and uterine expression of ER and PGHS-2 genes.

Key Words: uterus • gene • bovine somatotropin • receptor

Abbreviation key: ER = estrogen receptor alpha, PGHS = prostaglandin endoperoxide synthase, PPAR = peroxisome proliferator-activated receptor, TBS = Tris-buffered saline

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