HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Interpretive Summary Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Luna, P. Articles by de la Fuente, M. A. Search for Related Content PubMed PubMed Citation Articles by Luna, P. Articles by de la Fuente, M. A.

Validation of a Rapid Milk Fat Separation Method to Determine the Fatty Acid Profile by Gas Chromatography

Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria s/n. 28040 Madrid, Spain

Corresponding author: Miguel Angel de la Fuente; e-mail: ifraf91{at}if.csic.es.

An improved rapid method for separating lipids from milk to determine the fatty acid composition using 2 centrifugations at room temperature (20°C) was compared with the ISO-IDF reference procedure based on solvent extraction. The new method is useful for research and routine quality control and has a number of advantages over the reference procedure—mainly no solvents are required and it saves time. Applicability of the rapid separation method was confirmed in fats with different physical characteristics from ewe and goat milk samples. Minor differences were found in the proportions of some fatty acids in the reference and centrifugation methods. Milk fat separated by centrifugation at room temperature did not differ in fatty acid composition from milk centrifuged at 4°C.

Key Words: fatty acid • separation • milk fat • ewe

This article has been cited by other articles:

				P. Gomez-Cortes, P. Frutos, A. R. Mantecon, M. Juarez, M. A. de la Fuente, and G. Hervas Addition of Olive Oil to Dairy Ewe Diets: Effect on Milk Fatty Acid Profile and Animal Performance J Dairy Sci, August 1, 2008; 91(8): 3119 - 3127. [Abstract] [Full Text] [PDF]

				P. Gomez-Cortes, P. Frutos, A. R. Mantecon, M. Juarez, M. A. de la Fuente, and G. Hervas Milk Production, Conjugated Linoleic Acid Content, and In Vitro Ruminal Fermentation in Response to High Levels of Soybean Oil in Dairy Ewe Diet J Dairy Sci, April 1, 2008; 91(4): 1560 - 1569. [Abstract] [Full Text] [PDF]

				P. Luna, A. Bach, M. Juarez, and M. A. de la Fuente Effect of a Diet Enriched in Whole Linseed and Sunflower Oil on Goat Milk Fatty Acid Composition and Conjugated Linoleic Acid Isomer Profile J Dairy Sci, January 1, 2008; 91(1): 20 - 28. [Abstract] [Full Text] [PDF]