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J. Dairy Sci. 88:49-54
© American Dairy Science Association, 2005.

Exfoliation of Helicobacter pylori from Gastric Mucin by Glycopolypeptides from Buttermilk

M. Matsumoto1, K. Hara1, H. Kimata1, Y. Benno2 and C. Shimamoto3

1 Fundamental Research Laboratory, Kyodo Milk Industry Co. Ltd., Hirai, Hinode, Tokyo 190-0182, Japan
2 Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama, 351-0198, Japan
3 Department of Internal Medicine II, Osaka Medical College, Takatsuki, Osaka 569-8686, Japan

Corresponding author: M. Matsumoto; e-mail: m-matumoto{at}meito.co.jp.

In the human stomach, Helicobacter pylori, an ulcer pathogenic bacterium, colonizes the gastric mucosal layer primarily. The ability of glycopolypeptides (GPP) prepared from buttermilk to exfoliate H. pylori bound to gastric mucin was investigated. The GPP were prepared from buttermilk by digestion with trypsin, papain, pancreatin, bromelain, or pepsin. Helicobacter pylori ATCC 43504T and 43579 adhered more strongly to all of the GPP tested than to whole buttermilk, the soluble fraction of buttermilk, gastric mucin prepared from mouse stomach, or commercial pig gastric mucin. The GPP digested with trypsin, papain, or pancreatin were significantly more adherent. When the GPP concentration was 10 mg/mL, bound H. pylori ATCC 43504T, 43579, and 5 clinical isolates were exfoliated markedly from immobilized porcine gastric mucin following treatment with GPP digested with trypsin or pancreatin. This ability of GPP did not correlate with sialic acid content, indicating that sialic acid content is not important in the exfoliation of this microorganism. Such an ability may depend on the structure or number of sugar chains, or the position of sialic acid. We conclude that GPP promote the exfoliation of H. pylori bound to gastric mucin and prevent the de novo adherence of this microorganism. As such, GPP are a promising food material for preventing H. pylori infection.

Key Words: Helicobacter pylori • exfoliation • glycopolypeptide • buttermilk

Abbreviation key: GPP = glycopolypeptide, IC50 = 50% inhibitory concentration






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