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J. Dairy Sci. 87:2868-2873
© American Dairy Science Association, 2004.

Pregnancy Rates After Timed AI of Heifers Following Removal of Intravaginal Progesterone Inserts

I. D. Peeler1, R. L. Nebel1, R. E. Pearson1, W. S. Swecker2 and A. Garcia2

1 Department of Dairy Science and
2 Department of Large Animal Clinical Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061

Corresponding author: R. L. Nebel; e-mail: rnebel{at}vt.edu.

Reproductive performance of dairy heifers was compared for each of 2 synchronization protocols: The first group of 54 heifers was synchronized using intravaginal progesterone inserts (CIDR) plus estradiol cypionate (ECP) on d 0, PGF2{alpha} on d 7, and ECP again on d 8 (CIDR-ECP); a second group of 56 heifers was synchronized using CIDR and ECP on d 0, PGF2{alpha} on d 7, and GnRH on d 9 (CIDR-GnRH). All heifers received timed artificial insemination (TAI) at 48, 56, or 72 h after CIDR removal on d 7. Pregnancy diagnosis was conducted by ultrasonography 32 ± 1 d post AI to confirm pregnancy and at 60 ± 1 d post AI to determine embryo survival. Ovaries were monitored by ultrasonography daily from d 0 to 7 and twice daily from d 8 to ovulation to examine emergence of a new wave of follicles, size of the ovulatory follicle, and timing of ovulation on 15 heifers per protocol. New follicular development was detected 3.7 ± 0.2 d after CIDR insertion. Heifers receiving CIDR-ECP had a shorter interval from CIDR removal to ovulation than heifers receiving CIDR-GnRH (63.8 ± 3.0 vs. 71.6 ± 2.3 h, respectively); however, ovulation occurred 39.8 ± 3.0 h after ECP or 23.6 ± 2.3 h after GnRH. Diameters of ovulatory follicles did not differ between treatments. Overall pregnancy rate for synchronized heifers was 60.1%, and embryo survival was 98%. Pregnancy rate for heifers synchronized with CIDR-ECP was 63.0% and similar to that in heifers synchronized with CIDR-GnRH (57.1%). Pregnancy rate was affected by time of AI for heifers synchronized using CIDR-ECP but not for those synchronized with CIDR-GnRH. Heifers in the CIDR-ECP group that were inseminated 56 h after CIDR removal had a higher pregnancy rate (81.0%) compared with heifers inseminated 48 (66.7%) or 72 h (50.0%) after CIDR removal. Either ECP or GnRH used in a CIDR-based TAI program in dairy heifers can achieve acceptable reproductive performance.

Key Words: intravaginal progesterone insert • timed artificial insemination • reproductive performance • dairy heifer

Abbreviation key: CIDR = intravaginal progesterone insert, E2 = estrogen, ECP = estradiol cypionate, P4 = progesterone, PR = pregnancy rate, TAI = timed artificial insemination




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