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Structural Analysis of a New Anti-Hypertensive Peptide (ß-Lactosin B) Isolated from a Commercial Whey Product

¹ Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1, Tsutsumidori - Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
² Meiji Dairies Corporation, 540, Naruda, Odawara, Kanagawa 250-0862, Japan
³ Meiji Kenko Ham Co., Ltd., 5-21-12, Minamioi, Shinagawa-ku, Tokyo 140-0013, Japan

Corresponding author: T. Saito; e-mail: tsaito{at}bios.tohoku.ac.jp.

Angiotensin-converting enzyme (ACE) inhibitory activities and anti-hypertensive activities in spontaneously hypertensive rats (SHR) of 12 kinds of commercial peptides of food additive grade were measured. Four peptide products derived from milk proteins showed strong anti-hypertensive activities (>–18.0 mm Hg). A sample of WE80BG derived from whey proteins showed the strongest anti-hypertensive activity (–21.2 ± 16.9 mm Hg) with a medium level of ACE inhibitory activity (53.6%), and it was subjected to hydrophobic and gel filtration chromatography. From the low molecular weight fraction, an anti-hypertensive peptide was isolated by using reversed-phase HPLC, and it was found to be a tetrapeptide, alanine-leucine-proline-methionine (Ala-Leu-Pro-Met, ALPM), the origin of which was estimated to be ß-lactoglobulin f 142 to 145. At 8 h after oral administration of ALPM in SHR, systolic blood pressure was significantly decreased (–21.4 ± 7.8 mm Hg), but the IC₅₀ value (concentration of peptide needed to inhibit 50% of the ACE activity) of ALPM was not so high. We named the Ala-Leu-Pro-Met "ß-lactosin B." This peptide is the second anti-hypertensive peptide found from ß-lactoglobulin. Because WE80BG containing ALPM was also found to show the strongest anti-hypertensive activity (–24.5 ± 10 mm Hg) at 8 h after oral administration in SHR, WE80BG would be suitable for application to the development of a new food expected to have anti-hypertensive effects.

Key Words: whey • anti-hypertensive peptide • angiotensin-converting enzyme

Abbreviation key: ACE = angiotensin-converting enzyme, ALPM = Ala-Leu-Pro-Met, IC₅₀ = concentration of peptide needed to inhibit 50% of the ACE activity, RP = reversed phase, SBP = systolic blood pressure, SHR = spontaneously hypertensive rats, TFA = trifluoroacetic acid

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