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Differential Effects of Steroids and Retinoids on Bovine Myelopoiesis in Vitro

Department of Physiology, Biochemistry and Biometrics, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium

Corresponding author: C. Burvenich; e-mail: Christian.Burvenich{at}UGent.be.

Pregnancy and parturition are associated with physiological changes caused by steroid hormones. Alterations in number, maturity, and function of polymorphonuclear leukocytes observed in dairy cows at parturition suggest a common causative relationship with steroid hormones. This study was designed to investigate the effects of progesterone, 17-ß-estradiol, and hydrocortisone on the proliferation of bovine progenitor cells. An in vitro culturing system was used, and colonies were scored after 7 d of incubation. At low concentrations, 17-ß-estradiol inhibited proliferation of granulocyte progenitor cells. Hydrocortisone reduced growth of granulocyte and monocyte colonies, whereas myelopoiesis was not altered by progesterone. Furthermore, we studied the effect of retinoids on colony formation of bovine bone marrow cells. All-trans- and 9-cis-retinoic acid stimulated growth of granulocyte colonies and inhibited proliferation of the monocyte lineage. The addition of the 13-cis-isomer also increased numbers of granulocyte colony-forming units. This study indicates that steroid hormones may be responsible for alterations in the bovine hematopoietic profiles observed in circulation during the postpartum period. White blood cells, especially polymorphonuclear leukocytes, which are derived from bone marrow, are an important first line defense against mastitis. Therefore, these effects of steroids might contribute to the increased susceptibility of dairy cows to Escherichia coli mastitis. We furthermore hypothesize that an important role might be attributed to retinoic acid in its regulation of bovine myelopoiesis. Modulation of myelopoiesis in favor of the granulocyte lineage during the acute-phase reaction may be an adaptive mechanism designed to increase the capacity of first-line defense to intramammary infections.

Key Words: steroid hormone • retinoic acid • bone marrow • myelopoiesis

Abbreviation key: cfu-G = colony-forming unit-granulocyte, cfu-GM = colony-forming unit-granulocyte and monocyte, cfu-M = colony-forming unit-monocyte, cfu-total = colony-forming unit-total myeloid, FBS = fetal bovine serum, IMDM = Iscove’s modified Dulbecco’s Minimal Essential, PMN = polymorphonuclear leukocytes, RAR = retinoic acid receptor, RXR = retinoid X receptor

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