| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Animal and Veterinary Sciences, Clemson University, Clemson, SC 29634
Corresponding author: A. A. AbuGhazaleh; e-mail: aabugha{at}clemson.edu.
Previous studies found that feeding dairy cows a blend of fish and soybean oils enhanced milk vaccenic acid (VA) and conjugated linoleic acid (CLA) concentrations more than when the oils were fed separately. In these studies, the authors concluded that a component in fish oil was stimulating ruminal VA production from other sources of unsaturated fatty acids; however, that component was not identified. The objective of this study was to determine whether docosahexaenoic acid (DHA), an omega-3 fatty acid (FA) in fish oil, is the active component that promotes trans-C18:1 FA, VA in particular, accumulation using cultures of mixed ruminal microorganisms. Treatments consisted of control, control plus 5 mg of DHA (DH), control plus 30 mg of soybean oil (SBO), and control plus 5 mg of DHA and 30 mg of SBO (DHSBO). Treatments were incubated in triplicate in 125-mL flasks, and 5 mL of culture contents was taken at 0 and 24 h for fatty acid analysis by gas-liquid chromatography. After 24 h of incubation, the level of trans-C18:1 FA (14.1 and 11.7 mg/culture) and VA (13.0 and 10.2 mg/culture) increased more with added DHA than with added SBO, respectively. Combining DHA and SBO yielded higher quantities of trans-C18:1 FA (21.3 mg/culture) and VA (19.8 mg/culture) in the cultures than either fat source alone. These data suggest that DHA is the component in fish oil that promotes VA accumulation when incubated with linoleic acid.
Key Words: docosahexaenoic acid • soybean oil • vaccenic acid
Abbreviation key: CLA = conjugated linoleic acid, DH = control plus 5 mg of DHA, DHA = docosahexaenoic acid, DHBSO = control plus 5 mg of DHA and 30 mg of SBO, EPA = eicosapentaenoic acid, FA = fatty acid, SBO = soybean oil, VA = trans-11 vaccenic acid
This article has been cited by other articles:
|
|
 |
|
  V. R. Osborne, S. Radhakrishnan, N. E. Odongo, A. R. Hill, and B. W. McBride Effects of supplementing fish oil in the drinking water of dairy cows on production performance and milk fatty acid composition J Anim Sci, March 1, 2008; 86(3): 720 - 729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Flowers, S. A. Ibrahim, and A. A. AbuGhazaleh Milk Fatty Acid Composition of Grazing Dairy Cows When Supplemented with Linseed Oil J Dairy Sci, February 1, 2008; 91(2): 722 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. C. Jenkins, R. J. Wallace, P. J. Moate, and E. E. Mosley BOARD-INVITED REVIEW: Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem J Anim Sci, February 1, 2008; 86(2): 397 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Or-Rashid, J. K. G. Kramer, M. A. Wood, and B. W. McBride Supplemental algal meal alters the ruminal trans-18:1 fatty acid and conjugated linoleic acid composition in cattle J Anim Sci, January 1, 2008; 86(1): 187 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. AbuGhazaleh, D. O. Felton, and S. A. Ibrahim Milk Conjugated Linoleic Acid Response to Fish Oil and Sunflower Oil Supplementation to Dairy Cows Managed Under Two Feeding Systems J Dairy Sci, October 1, 2007; 90(10): 4763 - 4769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Castaneda-Gutierrez, M. J. de Veth, A. L. Lock, D. A. Dwyer, K. D. Murphy, and D. E. Bauman Effect of Supplementation with Calcium Salts of Fish Oil on n-3 Fatty Acids in Milk Fat J Dairy Sci, September 1, 2007; 90(9): 4149 - 4156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cruz-Hernandez, J. K. G. Kramer, J. J. Kennelly, D. R. Glimm, B. M. Sorensen, E. K. Okine, L. A. Goonewardene, and R. J. Weselake Evaluating the Conjugated Linoleic Acid and Trans 18:1 Isomers in Milk Fat of Dairy Cows Fed Increasing Amounts of Sunflower Oil and a Constant Level of Fish Oil J Dairy Sci, August 1, 2007; 90(8): 3786 - 3801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. AbuGhazaleh and W. R. Buckles The Effect of Solids Dilution Rate and Oil Source on Trans C18:1 and Conjugated Linoleic Acid Production by Ruminal Microbes in Continuous Culture J Dairy Sci, February 1, 2007; 90(2): 963 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. AbuGhazaleh, L. D. Holmes, B. N. Jacobson, and K. F. Kalscheur Short communication: Eicosatrienoic acid and docosatrienoic acid do not promote vaccenic acid accumulation in mixed ruminal cultures. J Dairy Sci, November 1, 2006; 89(11): 4336 - 4339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Whitlock, D. J. Schingoethe, A. A. AbuGhazaleh, A. R. Hippen, and K. F. Kalscheur Milk production and composition from cows fed small amounts of fish oil with extruded soybeans. J Dairy Sci, October 1, 2006; 89(10): 3972 - 3980. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
