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Agricultural Research Service, USDA, US Dairy Forage Research Center, Madison, WI 53706
Corresponding author: e-mail: gbroderi{at}dfrc.wisc.edu.
Effectiveness of low level monensin supplementation on N utilization in lactating dairy cows fed alfalfa silage was assessed using 48 multiparous Holsteins. Cows were fed a covariate diet [% of dry matter (DM): 56% alfalfa silage, 39% ground high moisture corn, 3% soybean meal, 1% ground corn, 1% vitamin-mineral supplements] for 2 wk, then grouped by days in milk into blocks of 4. Cows were randomly assigned within blocks to 1 of 4 diets that were fed for 10 wk: 1) control (covariate diet), 2) control plus 3% fish meal (replacing DM from high moisture corn), 3) monensin (10 mg/kg DM), and 4) monensin plus 3% fish meal. Diets 1 and 3 averaged 16.7% crude protein (25% from free AA in alfalfa silage); diets 2 and 4 averaged 18.5% crude protein. Monensin intake averaged 16 mg/d on diets 1 and 2 (due to contamination) and 248 mg/d on diets 3 and 4. There was no effect of fish meal or monensin on DM intake. However, weight gain and yield of milk, protein, and SNF increased with fish meal feeding, indicating metabolizable protein limited production. Feeding monensin increased blood glucose but reduced yield of 3.5% fat-corrected milk, milk fat content and yield, and milk protein content and yield. Apparent N efficiency was greatest on monensin (diet 3) but lowest on monensin plus fish meal (diet 4). Fish meal reduced blood glucose concentration and apparent N efficiency, and increased concentrations of milk and blood urea. Monensin increased ruminal propionate concentration and decreased concentration of acetate and butyrate and acetate:propionate in ruminally cannulated cows fed the experimental diets. However, these changes were small, suggesting that too little monensin was fed. Fish meal reduced ruminal total amino acid (AA) but monensin did not alter ruminal NH3 or total AA. Both fish meal and monensin increased NH3 formation from casein AA using ruminal inoculum from the cannulated cows. There was no evidence from this trial that feeding 250 mg of monensin per day to lactating cows improved N utilization by reducing ruminal catabolism of the large amounts of free AA in alfalfa silage.
Key Words: monensin • fish meal • milk yield • N efficiency
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