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Department of Animal Sciences, University of Florida, Gainesville 32611
Corresponding author: A. T. Adesogan; e-mail: adesogan{at}animal.ufl.edu.
This study determined the effectiveness of an inoculant (BB), molasses, or a mixture of either BB and molasses (BBM) or BB and fibrolytic enzymes (BBE) for improving the fermentation and aerobic stability of bermudagrass. A 6-wk regrowth of Tifton 85 bermudagrass was conserved in quadruplicate mini silos alone or after treatment application. The inoculant contained a mixture of P. pentosaceus 12455, 1 x105 cfu/g of fresh forage, L. buchneri 40788, 4 x105 cfu/g of fresh forage, and beta-glucanase, alpha-amylase, and xylanase; BBE contained similar bacteria and enzymes as BB, but greater enzyme activities. Chemical composition was quantified after 2, 4, 7, 30, and 60 d of ensiling. Microbial composition and aerobic stability were measured after 60 d of ensiling, at which point the pH of additive-treated silages was consistently lower and DM recovery was higher than in untreated silages. The BB, BBM, and molasses-treated silages had less ammonia N than untreated silages, and BB, BBM, and BBE-treated silages had less residual water-soluble carbohydrates than untreated silages. All silages had high acetic acid (47.5 g/kg DM) and low lactic acid (1.7 g/kg DM) concentrations. However, untreated and BBE-treated silages had more butyric acid and ammonia N, suggesting that a clostridial fermentation had occurred. These butyric forages were more aerobically stable (27 d) but less desirable for feeding than those ensiled with BB or molasses, which were stable for 6.9 d. In conclusion, BB and molasses treatments improved the digestibility and fermentation of bermudagrass and produced higher quality silages that were stable for 6.9 d. Mixing BB with molasses or the inoculant tested was not more beneficial than BB or molasses alone.
Key Words: Lactobacillus buchneri aerobic stability inoculant molasses
Abbreviation key: BB = a mixture of Pediococcus pentosaceus 12455, 1 x105 cfu/g of fresh forage, Lactobacillus buchneri 40788, 4 x105 cfu/g of fresh forage, and beta-glucanase, alpha-amylase, and xylanase, BBE = BB plus additional beta-glucanase, alpha-amylase, and xylanase, BBM = BB plus molasses, LAB = lactic acid bacteria, WSC = water-soluble carbohydrates.
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