HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Interpretive Summary Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lamote, I. Articles by Burvenich, C. Search for Related Content PubMed PubMed Citation Articles by Lamote, I. Articles by Burvenich, C.

Influence of 17ß-Estradiol, Progesterone, and Dexamethasone on Diapedesis and Viability of Bovine Blood Polymorphonuclear Leukocytes

Department of Physiology, Biochemistry and Biometrics, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium

Corresponding author: C. Burvenich; e-mail: christian.burvenich{at}ugent.be.

The aim of the current study was to investigate whether polymorphonuclear leukocyte (PMN) diapedesis and viability are influenced by steroid hormones. Using an in vitro model with different types of cell layers (bovine mammary epithelial cells and fibroblasts), we investigate whether steroid hormone treatments (17ß-estradiol, progesterone, and dexamethasone) have an influence on the diapedesis capacity and viability of PMN. In addition, we studied apoptosis of PMN in the in vitro model and evaluated the influence of different types of cell layers and steroid hormone treatments on this process. A significant decrease in the number of viable PMN in the lower compartment of the in vitro model (i.e., number of migrated PMN x viability after migration) was found after 17ß-estradiol treatment, whereas no influence was detected after progesterone or dexamethasone treatment. The effect of 17ß-estradiol was not due to a lower viability before migration as none of the treatments caused a significant effect on the viability before diapedesis. This treatment effect was not influenced by endogenous 17ß-estradiol or progesterone levels before isolation because there was no correlation between these plasma levels and PMN diapedesis capacity or viability. Furthermore, migration through epithelial cells caused a significant decrease in viability of PMN due to increased apoptosis but not necrosis.

Key Words: steroid • diapedesis • viability • polymorphonuclear leukocyte

Abbreviation key: BME = bovine mammary epithelial, HBSS = Hank’s balanced salt solution, PI = propidium iodide, PMN = polymorphonuclear leukocytes, ZAS = zymosan-activated serum.

This article has been cited by other articles:

				M. C. Loiselle, C. Ster, B. G. Talbot, X. Zhao, G. F. Wagner, Y. R. Boisclair, and P. Lacasse Impact of postpartum milking frequency on the immune system and the blood metabolite concentration of dairy cows J Dairy Sci, May 1, 2009; 92(5): 1900 - 1912. [Abstract] [Full Text] [PDF]

				S. Piepers, S. De Vliegher, K. Demeyere, B. N. Lambrecht, A. de Kruif, E. Meyer, and G. Opsomer Technical note: Flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability J Dairy Sci, February 1, 2009; 92(2): 626 - 631. [Abstract] [Full Text] [PDF]

				C. Burvenich, D. D. Bannerman, J. D. Lippolis, L. Peelman, B. J. Nonnecke, M. E. Kehrli Jr., and M. J. Paape Cumulative Physiological Events Influence the Inflammatory Response of the Bovine Udder to Escherichia coli Infections During the Transition Period J Dairy Sci, June 1, 2007; 90(13_suppl): E39 - E54. [Abstract] [Full Text] [PDF]