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J. Dairy Sci. 87:3235-3237
© American Dairy Science Association, 2004.

Short Communication: Amino Acid Transporters in Porcine Mammary Gland During Lactation*

J. Pérez Laspiur1, J. L. Burton1, P. S. D. Weber1, R. N. Kirkwood2 and N. L. Trottier1

1 Department of Animal Science and
2 College of Veterinary Medicine, Michigan State University, East Lansing 48824

Corresponding author: N. L. Trottier; e-mail: trottier{at}msu.edu.

The objective of this study was to determine whether mRNA transcripts for the amino acid (AA) transporter proteins CAT-1, CAT-2B, B0,+, and ASCT1 are present in porcine mammary tissue (MT). Transcript abundance on d 7 and 17 of lactation was determined by Northern blot analysis, and absolute quantification was performed by real-time polymerase chain reaction. Porcine MT expresses CAT-1, CAT-2B, B0,+, and ASCT1 during lactation. Preliminary findings indicate that B0,+ mRNA abundance tended to decrease on d 17 compared with that on d 7.

Key Words: amino acid transporter • mammary gland • lactation • porcine

Abbreviation key: MT = mammary tissue.






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