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1 Department of Animal Science and
2 College of Veterinary Medicine, Michigan State University, East Lansing 48824
Corresponding author: N. L. Trottier; e-mail: trottier{at}msu.edu.
The objective of this study was to determine whether mRNA transcripts for the amino acid (AA) transporter proteins CAT-1, CAT-2B, B0,+, and ASCT1 are present in porcine mammary tissue (MT). Transcript abundance on d 7 and 17 of lactation was determined by Northern blot analysis, and absolute quantification was performed by real-time polymerase chain reaction. Porcine MT expresses CAT-1, CAT-2B, B0,+, and ASCT1 during lactation. Preliminary findings indicate that B0,+ mRNA abundance tended to decrease on d 17 compared with that on d 7.
Key Words: amino acid transporter mammary gland lactation porcine
Abbreviation key: MT = mammary tissue.
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J. P. Laspiur, J. L. Burton, P. S. D. Weber, J. Moore, R. N. Kirkwood, and N. L. Trottier Dietary Protein Intake and Stage of Lactation Differentially Modulate Amino Acid Transporter mRNA Abundance in Porcine Mammary Tissue J. Nutr., September 1, 2009; 139(9): 1677 - 1684. [Abstract] [Full Text] [PDF] |
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