HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Jiang, Y. J. Articles by Geng, L. J. Search for Related Content PubMed PubMed Citation Articles by Jiang, Y. J. Articles by Geng, L. J.

Expression and Bioactivity Analysis of Recombinant Beta-CPP Dimer

Life Science and Biotechnique Research Center, Northeast Agricultural University, Harbin, Heilongjiang, 150030 China

Corresponding author: Q. Z. Li; e-mail:qingzhangli{at}hotmail.com.

Beta-casein phosphopeptide (beta-CPP) is a bioactive peptide that carries different minerals, especially calcium. To investigate more effects of beta-CPP, eukaryotic expression vector of beta-CPP dimer was constructed and transfected into Chinese hamster ovary (CHO) cells. After selection, the cell lines stably expressing beta-CPP dimer were obtained, and the recombinant product was identified and purified. Activity assay of recombinant protein indicated that the recombinant beta-CPP dimer could improve Ca²⁺ uptake of sperm, stimulate the proliferation of spleen cells, and induce apoptosis of some malignant tumor cells.

Key Words: beta-casein phosphopeptide • dimer • eukaryotic expression • bioactivity

Abbreviation key: [Ca²⁺]_i = intracellular free Ca²⁺ concentration, CHO = Chinese hamster ovary, CNBr = cyanogen bromide, Con A = concanavalin A, CPP = casein phosphopeptide, FBS = fetal bovine serum.