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J. Dairy Sci. 86:1472-1475
© American Dairy Science Association, 2003.

Technical Note: Use of RFLP to Characterize Lactococcus lactis Strains Producing Exopolysaccharides

H. Deveau and S. Moineau

Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire Université Laval, Québec, Canada, G1K 7P4

Corresponding author:
S. Moineau; e-mail:
Sylvain.Moineau{at}bcm.ulaval.ca.

Restriction fragment length polymorphism (RFLP) is used to differentiate microorganisms by analysis of their DNA restriction patterns. A modified RFLP procedure is proposed for the rapid characterization of Lactococcus lactis strains producing exopolysaccharides (EPS). The availability of such effective cataloging system is likely to benefit research aimed at identifying lactococcal strains that produce novel EPS.

Key Words: exopolysaccharide • Lactococcus lactis • restriction fragment length polymorphism

Abbreviation key: EPS = exopolysaccharide, EPS+ = ability to produce exopolysaccharides, RFLP = restriction fragment length polymorphism




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