JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tyler, P. J.
Right arrow Articles by Cummins, K. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tyler, P. J.
Right arrow Articles by Cummins, K. A.
J. Dairy Sci. 86:622-629
© American Dairy Science Association, 2003.

Effect of Dietary Ascorbyl-2-Phosphate on Immune Function after Transport to a Feeding Facility

P. J. Tyler and K. A. Cummins1

Department of Animal Sciences, Alabama Agricultural Experiment Station, Auburn University, AL 36849

1 Corresponding author:
K. A. Cummins; e-mail:
kcummins{at}acesag.auburn.edu.

Effects of dietary ascorbyl-2-phosphate on immune function after a 210-km trip were measured in 18 Holstein heifers. After transport on d 0, 10 g of ascorbyl-2-phosphate each were added to the diets of 10 heifers, whereas eight heifers were fed a control diet. Plasma cortisol concentrations increased by an average of 25.6 µg/ml on d 0 following transport, but by d 7 after transport had decreased to pretransport levels. Average daily gain was lower in heifers fed ascorbyl-2-phosphate from d 28 to 49 d after transport, but did not differ over the entire study. Feeding ascorbyl-2-phosphate maintained plasma ascorbate concentrations on d 7 post-transport, which decreased in control heifers. Plasma keyhole limpet hemocyanin antibody titers were significantly higher in control heifers from d 7 to 49. Mononuclear leukocyte proliferation responses were decreased on d 0 in lymphocytes stimulated by mitogens, with pokeweed mitogen-stimulated cells showing less of a response than cells stimulated by the other mitogens. In the absence of mitogens, dietary ascorbyl-2-phosphate increased basal 3H-methyl thymidine incorporation by cultured lymphocytes. Across diets and mitogens, lymphocytes treated with cortisol showed decreased 3H-methyl thymidine incorporation. Transportation acted as a stressor, as evidenced by the increased plasma cortisol levels at d 0 immediately after transport, but immunological effects were not apparent by d 7. Feeding ascorbyl-2-phosphate maintained plasma ascorbate concentrations on d 7, but had negative effects on immune responses posttransport.

Key Words: ascorbic acid • cattle • immune function

Abbreviation key: A2P = Ascorbyl-2-phosphate magnesium, A2PP = ascorbyl-2-polyphosphate, ConA = concanavalin A, cortisol = hydrocortisone-21-phosphate, HBSS = Hank’s balanced salt solution, KLH = keyhole limpet hemocyanin, MNL = mononuclear lymphocytes, PHA = phytohemagglutinin, PWM = pokeweed mitogen




This article has been cited by other articles:


Home page
 J ANIM SCIHome page
L. Padilla, T. Matsui, S. Ikeda, M. Kitagawa, and H. Yano
The effect of vitamin C supplementation on plasma concentration and urinary excretion of vitamin C in cattle
J Anim Sci, December 1, 2007; 85(12): 3367 - 3370.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
K. J. Stanger, N. Ketheesan, A. J. Parker, C. J. Coleman, S. M. Lazzaroni, and L. A. Fitzpatrick
The effect of transportation on the immune status of Bos indicus steers
J Anim Sci, November 1, 2005; 83(11): 2632 - 2636.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Dairy Science Association ®.