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Adaptations in Body Muscle and Fat in Transition Dairy Cattle Fed Differing Amounts of Protein and Methionine Hydroxy Analog¹

G. J. Phillips^*, T. L. Citron, J. S. Sage, K. A. Cummins⁺, M. J. Cecava^# and J. P. McNamara

^* CH2M Hill, Hanford, WA
Purina Mills, Inc., PA
Yerrington, NV
⁺ Auburn University, Auburn, AL
^# Consolidated Nutrition, Decatur, IN
Department of Animal Sciences, Washington State University, Pullman 99163

Corresponding author: J. P. McNamara; e-mail: mcnamara{at}wsu.edu.

The objectives were to determine effects of prepartum protein intake and dietary amino acid balance on production, adaptations in body fat and protein, amino acid concentrations, and, indirectly, body protein breakdown in early lactation. Multiparous Holstein cows (n = 42) were fed diets containing 11 or 14% crude protein with or without 20 g/d of methionine hydroxy analog for 21 d prepartum and then fed a common diet of 17% crude protein for 120 d postpartum, with or without 50 g/d of methionine hydroxy analog. Dry matter intake postpartum averaged 25.4 kg and milk production 41.6 kg. Cows fed the 14% CP diet ate 0.7 kg more dry matter and gave 1.7 kg more milk than those fed the 11% diet postpartum, but this difference was not significant. Cows fed methionine hydroxy analog prepartum lost less body protein from -14 to 60 d in milk. From d 60 to 120, body fat increased 8.5 and 11.5 kg for low and high protein groups and body protein increased 0.5 and 1.0 kg. Serum concentrations of branched chain amino acids fell 17% in the first few weeks postpartum, lysine fell 15%, histidine fell 16%, methionine increased 20%, and cysteine increased 30%. The ratio of serum 3-methylhistidine to creatinine was determined to indicate muscle protein degradation. An increase in this ratio at 7 d postpartum indicated increased body protein breakdown, there was no effect of prepartum ration. Increased protein intake prepartum may allow more feed intake and milk production postpartum, and supplementing a methionine analog on a ration already balanced in methionine by contemporary models may spare body protein.

Key Words: methionine analog • body fat • body protein • transition

Abbreviation key: BF = body fat, BP = body protein, HMB = D,L-2-hydroxy-4-(methylthio)-butanoic acid, MHA = methionine hydroxy analog, MP = metabolizable protein, 3MH = 3-methyl histidine

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