HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Waldron, M. R. Articles by Overton, T. R. Search for Related Content PubMed PubMed Citation Articles by Waldron, M. R. Articles by Overton, T. R.

Effect of Lipopolysaccharide Infusion on Serum Macromineral and Vitamin D Concentrations in Dairy Cows¹

M. R. Waldron^*, B. J. Nonnecke, T. Nishida^*, R. L. Horst and T. R. Overton^*

^* Department of Animal Science, Cornell University, Ithaca 14853
National Animal Disease Center (NADC), USDA.ARS, Ames 50010

Corresponding author: Thomas R. Overton; e-mail: tro2{at}cornell.edu.

Four multiparous lactating cows (175 to 220 d in milk) were used in a 4 x 4 Latin square design to assess the effects of four doses (0.0, 0.5, 1.0, 1.5 µg/kg of body weight) of lipopolysaccharide (LPS; Escherichia coli 0111:B4) on circulating concentrations of macrominerals and vitamin D metabolites. Treatments were dissolved in 100 ml of sterile saline and infused intravenously over a period of 100 min. Blood was sampled immediately before infusion (0 h), at 60-min intervals for 8 h, and at 24 and 48 h postinfusion. Vitamin D metabolites were analyzed in samples collected at 0, 2, 6, 24, and 48 h only. Serum Ca and P concentrations decreased after LPS infusion, but there was no effect on serum magnesium concentration. Plasma 25-OH vitamin D₃ and 1,25-(OH)₂ vitamin D₃ were not affected by LPS infusion; however, when analyzed as 0 vs. all other doses of LPS combined, there was a tendency for plasma 1,25-(OH)₂ vitamin D₃ concentration to decrease when cows were infused with LPS. The inflammatory response elicited by LPS altered plasma macromineral concentrations, a result that may have important implications for calcium homeostasis and metabolic health of lactating dairy cows.

Key Words: lipopolysaccharide • minerals • vitamin D

Abbreviation key: 1,25-(OH)₂D = 1, 25-dihydroxyvitamin D₃, 25-OHD = 25-hydroxyvitamin D₃, PIH = hour after the initiation of infusion, PTH = parathyroid hormone, TNF- = tumor necrosis factor-

This article has been cited by other articles:

				T. R. Overton and M. R. Waldron Nutritional Management of Transition Dairy Cows: Strategies to Optimize Metabolic Health J Dairy Sci, July 1, 2004; 87(13_suppl): E105 - 119. [Abstract] [Full Text] [PDF]

				M. R. Waldron, T. Nishida, B. J. Nonnecke, and T. R. Overton Effect of Lipopolysaccharide on Indices of Peripheral and Hepatic Metabolism in Lactating Cows J Dairy Sci, November 1, 2003; 86(11): 3447 - 3459. [Abstract] [Full Text] [PDF]