HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pengov, A. Articles by Ceru, S. Search for Related Content PubMed PubMed Citation Articles by Pengov, A. Articles by Ceru, S.

Antimicrobial Drug Susceptibility of Staphylococcus aureus Strains Isolated from Bovine and Ovine Mammary Glands

Institute for microbiology, Faculty of Veterinary Medicine, 1000 Ljubljana, Gerbiceva 60, Slovenia

Corresponding author: A. Pengov; e-mail: Andrej.Pengov{at}vf.uni-lj.si.

The activity of selected antimicrobial agents against Staphylococcus aureus was determined with the agar disk diffusion test to determine the diameter of the zone of inhibition and the E-test for determination of the minimal inhibitory concentration (MIC). The 92 S. aureus strains used in this study were isolated from bovine (n = 76) and ovine (n = 16) intramammary infections. Four antibiotics, which are frequently used in mastitis therapy were chosen: penicillin-G, ampicillin, kanamycin, and cephalexine. The fifth compound (oxacillin) was used to detect methicillin-resistant S. aureus, but no such strains could be found. According to the evaluation criteria, 65.2 (penicillin) to 93.5% (kanamycin, cephalexine) S. aureus strains were susceptible to the antibiotics tested. Ovine S. aureus strains reveal a lower resistance rate than bovine isolates. Comparison of the results of the two methods of susceptibility testing shows, with exception of penicillin and ampicillin, satisfactory agreement. Analyzing the results of the MIC endpoints and the zone diameter values, very major errors, according to the error rate bounded method of Metzler and DeHaan, occurred at an error rate of 3.3% for penicillin and 3.8% for ampicillin.

Key Words: antimicrobial drug susceptibility • Staphylococcus aureus • mastitis

Abbreviation key: MIC = minimal inhibitory concentration, NCCLS = National Committee for Clinical Laboratory Standards

This article has been cited by other articles:

				S. F. Nunes, R. Bexiga, L. M. Cavaco, and C. L. Vilela Technical Note: Antimicrobial Susceptibility of Portuguese Isolates of Staphylococcus aureus and Staphylococcus epidermidis in Subclinical Bovine Mastitis J Dairy Sci, July 1, 2007; 90(7): 3242 - 3246. [Abstract] [Full Text] [PDF]

				P. Moroni, G. Pisoni, M. Antonini, R. Villa, P. Boettcher, and S. Carli Short communication: antimicrobial drug susceptibility of Staphylococcus aureus from subclinical bovine mastitis in Italy. J Dairy Sci, August 1, 2006; 89(8): 2973 - 2976. [Abstract] [Full Text] [PDF]

				L. Guler, U. Ok, K. Gunduz, Y. Gulcu, and H. H. Hadimli Antimicrobial Susceptibility and Coagulase Gene Typing of Staphylococcus aureus Isolated from Bovine Clinical Mastitis Cases in Turkey J Dairy Sci, September 1, 2005; 88(9): 3149 - 3154. [Abstract] [Full Text] [PDF]